Abstract
Cysteine proteinases are involved in various physiological and developmental processes in plants. Two cDNAs from senescent and non-senescent tobacco leaves were isolated with degenerate primers designed from conserved regions of plant senescence-associated cysteine proteinases using rapid amplification of cDNA ends (RACE). Both sequences encode papain-like cysteine proteinases: the 833 bp fragment (NtCP1) encoding a C-terminus partial sequence of a putative tobacco cysteine proteinase gene whereas the 1300 bp fragment (NtCP2) is a full-length cysteine proteinase. On the amino acid sequence level, NtCP1 has a high similarity with other senescence-associated cysteine proteinases. It is expressed only in senescent leaves. It is not induced in mature green leaves upon exposure to drought or heat. These results suggest that it might be a good developmental senescence marker in tobacco. By contrast, NtCP2 has a high similarity to KDEL-tailed cysteine proteinases and is expressed in mature green leaves. Both drought and heat decreased NtCP2 transcript abundance in mature green leaves. It is concluded that NtCP1 is a senescence-specific cysteine proteinase whereas NtCP2 fulfils roles in green leaves that might be similar to those of KDEL-tailed cysteine proteinases involved, for example, in programmed cell death.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
NtCP1 is a partial putative tobacco cysteine proteinase sequence of 833 bp.
- Taxon
- tobacco
Nicotiana tabacume0 - Gene
- NtCP1e0
- Outcome
- C-terminus partial sequencee0
- Value
- 833 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the 833 bp fragment (NtCP1) encoding a C-terminus partial sequence of a putative tobacco cysteine proteinase gene”
Claim · model-written summary
NtCP2 is a full-length cysteine proteinase sequence of 1300 bp.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- NtCP2e0
- Outcome
- full-length cysteine proteinasee0
- Value
- 1300 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the 1300 bp fragment (NtCP2) is a full-length cysteine proteinase”
t0“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
NtCP1 has high amino acid similarity to other senescence-associated cysteine proteinases.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- NtCP1e0
- Outcome
- high similarity with other senescence-associated cysteine proteinasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NtCP1 has a high similarity with other senescence-associated cysteine proteinases”
t0“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
NtCP1 is expressed only in senescent tobacco leaves.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- senescent leavese0
- Gene
- NtCP1e1
- Outcome
- expressed only in senescent leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It is expressed only in senescent leaves”
e1“NtCP1 has a high similarity with other senescence-associated cysteine proteinases”
t0“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
Drought did not induce NtCP1 in mature green tobacco leaves.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- mature green leavese0
- Treatment
- droughte0
- Gene
- NtCP1e1
- Outcome
- inducede0
- Direction
- not inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It is not induced in mature green leaves upon exposure to drought or heat”
e1“NtCP1 has a high similarity with other senescence-associated cysteine proteinases”
t0“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
Heat did not induce NtCP1 in mature green tobacco leaves.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- mature green leavese0
- Treatment
- heate0
- Gene
- NtCP1e1
- Outcome
- inducede0
- Direction
- not inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It is not induced in mature green leaves upon exposure to drought or heat”
e1“NtCP1 has a high similarity with other senescence-associated cysteine proteinases”
t0“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
NtCP1 may be a developmental senescence marker in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Gene
- NtCP1e1
- Outcome
- developmental senescence markere0
- Qualifier
- mighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it might be a good developmental senescence marker in tobacco”
e1“NtCP1 has a high similarity with other senescence-associated cysteine proteinases”
Claim · model-written summary
NtCP2 is similar to KDEL-tailed cysteine proteinases and is expressed in mature green tobacco leaves.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- mature green leavese0
- Gene
- NtCP2e0
- Outcome
- high similarity to KDEL-tailed cysteine proteinases and is expressed in mature green leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NtCP2 has a high similarity to KDEL-tailed cysteine proteinases and is expressed in mature green leaves”
t0“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
Drought decreased NtCP2 transcript abundance in mature green tobacco leaves.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- mature green leavese0
- Treatment
- droughte0
- Gene
- NtCP2e0
- Outcome
- NtCP2 transcript abundancee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both drought and heat decreased NtCP2 transcript abundance in mature green leaves”
t0“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
Heat decreased NtCP2 transcript abundance in mature green tobacco leaves.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- mature green leavese0
- Treatment
- heate0
- Gene
- NtCP2e0
- Outcome
- NtCP2 transcript abundancee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both drought and heat decreased NtCP2 transcript abundance in mature green leaves”
t0“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
The authors infer that NtCP2 may have roles in green leaves similar to KDEL-tailed cysteine proteinases involved in programmed cell death.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- NtCP2e0
- Outcome
- roles in green leavese0
- Qualifier
- might be similar to those of KDEL-tailed cysteine proteinases involved, for example, in programmed cell deathe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NtCP2 fulfils roles in green leaves that might be similar to those of KDEL-tailed cysteine proteinases involved, for example, in programmed cell death”
t0“tobacco (Nicotiana tabacum L.)”
The same questions in other papers
heat · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
3 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →drought · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
6 papers in the same direction as this paper · 9 in the opposite direction
- positive
- positive
- positive
12 papers more in this group
See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.