Abstract
Environmental stress elevates the level of jasmonic acid (JA) and activates the biosynthesis of nicotine and related pyridine alkaloids in tobacco (Nicotiana tabacum L.) by up-regulating the expression of putrescine N-methyltransferase 1 (NtPMT1), which encodes a putrescine N-methyl transferase that catalyzes nicotine formation. The JA signal suppressor JASMONATE ZIM DOMAIN 1 (NtJAZ1) and its target protein, NtMYC2a, also regulate nicotine biosynthesis; however, how these proteins interact to regulate abiotic-induced nicotine biosynthesis is poorly understood. In this study, we found that high-temperature (HT) treatment activated transcription of NtMYC2a, which subsequently stimulated the transcription of genes associated with JA biosynthesis, including Lipoxygenase (LOX), Allene oxide synthase (AOS), Allene oxide cyclase (AOC), and 12-oxophytodienodate reductase (OPR). Overexpression of NtMYC2a increased nicotine biosynthesis by enhancing its binding to the promoter of NtPMT1. Overexpression of either NtJAZ1 or proteasome-resistant NtJAZ1∆C suppressed nicotine production under normal conditions, but overexpression only of the former resulted in low levels of nicotine under HT treatment. These data suggest that HT induces NtMYC2a accumulation through increased transcription to activate nicotine synthesis; meanwhile, HT-induced NtMYC2a can activate JA synthesis to promote additional NtMYC2a activity by degrading NtJAZ1 at the post-transcriptional level.
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Claims 8
Claim · model-written summary
High-temperature treatment activated NtMYC2a transcription in tobacco.
- Taxon
- tobacco
Nicotiana tabacume1 - Treatment
- high-temperature (HT) treatmente0
- Gene
- NtMYC2ae0
- Outcome
- transcription of NtMYC2ae0
- Direction
- activatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high-temperature (HT) treatment activated transcription of NtMYC2a”
e1“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
NtMYC2a stimulated transcription of LOX, AOS, AOC and OPR genes associated with JA biosynthesis.
- Taxon
- tobaccoNicotiana tabacume2
- Gene
- NtMYC2ae1
- Outcome
- transcription of genes associated with JA biosynthesise0
- Qualifier
- Lipoxygenase (LOX), Allene oxide synthase (AOS), Allene oxide cyclase (AOC), and 12-oxophytodienodate reductase (OPR)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which subsequently stimulated the transcription of genes associated with JA biosynthesis, including Lipoxygenase (LOX), Allene oxide synthase (AOS), Allene oxide cyclase (AOC), and 12-oxophytodienodate reductase (OPR)”
e1“high-temperature (HT) treatment activated transcription of NtMYC2a”
e2“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
NtMYC2a overexpression increased nicotine biosynthesis through enhanced binding to the NtPMT1 promoter.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- Overexpression of NtMYC2ae0
- Gene
- NtMYC2ae0
- Outcome
- nicotine biosynthesise0
- Direction
- increasede0
- Qualifier
- binding to the promoter of NtPMT1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of NtMYC2a increased nicotine biosynthesis by enhancing its binding to the promoter of NtPMT1”
e1“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
NtJAZ1 overexpression suppressed nicotine production under normal conditions.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- Overexpression of either NtJAZ1e0
- Gene
- NtJAZ1e0
- Outcome
- nicotine productione0
- Direction
- suppressede0
- Qualifier
- under normal conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of either NtJAZ1 or proteasome-resistant NtJAZ1∆C suppressed nicotine production under normal conditions”
e1“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
Proteasome-resistant NtJAZ1∆C overexpression suppressed nicotine production under normal conditions.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- proteasome-resistant NtJAZ1∆Ce0
- Gene
- NtJAZ1∆Ce0
- Outcome
- nicotine productione0
- Direction
- suppressede0
- Qualifier
- under normal conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of either NtJAZ1 or proteasome-resistant NtJAZ1∆C suppressed nicotine production under normal conditions”
e1“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
Under high temperature, NtJAZ1 overexpression resulted in low nicotine levels.
- Taxon
- tobaccoNicotiana tabacume2
- Treatment
- Overexpression of either NtJAZ1e1
- Gene
- NtJAZ1e1
- Outcome
- levels of nicotinee0
- Direction
- lowe0
- Qualifier
- under HT treatmente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression only of the former resulted in low levels of nicotine under HT treatment”
e1“Overexpression of either NtJAZ1 or proteasome-resistant NtJAZ1∆C”
e2“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
High temperature is proposed to increase NtMYC2a accumulation through transcription and activate nicotine synthesis.
- Taxon
- tobaccoNicotiana tabacume1
- Gene
- NtMYC2ae0
- Outcome
- nicotine synthesise0
- Direction
- activatee0
- Qualifier
- through increased transcriptione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HT induces NtMYC2a accumulation through increased transcription to activate nicotine synthesis”
e1“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
High-temperature-induced NtMYC2a is proposed to activate JA synthesis, further promoting NtMYC2a activity through post-transcriptional NtJAZ1 degradation.
- Taxon
- tobaccoNicotiana tabacume1
- Gene
- NtMYC2ae0
- Outcome
- JA synthesise0
- Direction
- activatee0
- Qualifier
- by degrading NtJAZ1 at the post-transcriptional levele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HT-induced NtMYC2a can activate JA synthesis to promote additional NtMYC2a activity by degrading NtJAZ1 at the post-transcriptional level”
e1“tobacco (Nicotiana tabacum L.)”
The same questions in other papers
heat · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
4 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- no effect
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.