Abstract
Plants inhabiting adverse growth conditions compete against stresses by the endogenous regulatory elements viz ., promoters and terminators at the ‘right time’ (time-of-stress-act), ‘right place’ (tissue-of-act), and ‘right expression’ (time-of-transcription). Heat stress at the reproductive stage impedes pollen viability and stigma receptiveness, affecting the seed set. Prosopis cineraria, the dominant desert-inhabiting legume tree, is heat- and drought-tolerant. The distribution of heat shock elements in a heat-inducible promoter determines the magnitudes of target gene expression in different tissues/organs. Relative expression of P. cineraria heat shock protein 18.2 ( PcHsp18.2 ) in alternate months of 2021 displayed the highest expression in summer. The flowers collected in June, the hottest month (47 o C) of 2021, exhibited a high expression of PcHsp18.2. The germination of the pollen collected was 80%, and the trees eventually set seeds. Comprehensive analysis of the promoter ( pPcHSP18.2 ) and terminator ( tPchsp18.2 ) of PcHsp18.2 by expressing the gusA in tobacco exhibited the highest expression under heat stress as similar to the expression of PcHsp18.2 in environmental samples. Ectopic expression of gusA under PcHsp18.2 promoter and terminator resulted in an increased seed set due to the viability of pollen and stigma under heat stress. The gusA expression under PcHsp18.2 promoter and terminator was high-fold in anther compared to the Lat52 and g10 (endogenous genes under its promoter and terminator) genes under heat stress. The expression of genes under strong and balanced endogenous inducible promoter and terminator combinations, as in the desert-growing P. cineraria in transgenic plants, enables the development of resilient crops. • Prosopis cineraria, a drought-tolerant desert legume tree, alleviates stresses by inducing endogenous regulatory elements. • Relative expression of P. cineraria Hsp18.2 displayed the highest expression in summer. • Heat tolerance during the reproductive stage favors pollen viability and stigma receptiveness to set seeds. • Heat stress results in high-fold gusA expression in anthers under endogenous promoter and terminator of PcHsp18.2 . • Transgenesis with strong and balanced inducible promoter and terminator combinations as in P. cineraria enable to develop resilient crops.
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Claims 9
Claim · model-written summary
P. cineraria PcHsp18.2 expression was highest in summer.
- Taxon
- P. cineraria
Prosopis cinerariae0 - Gene
- P. cineraria heat shock protein 18.2e0
- Outcome
- expressione0
- Direction
- higheste0
- Qualifier
- in summere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Relative expression of P. cineraria heat shock protein 18.2 ( PcHsp18.2 ) in alternate months of 2021 displayed the highest expression in summer.”
Claim · model-written summary
June flowers exhibited high PcHsp18.2 expression at 47 o C.
- Taxon
- Prosopis cinerariaProsopis cinerariat0
- Organ / stage
- flowerse0
- Gene
- PcHsp18.2e0
- Outcome
- expressione0
- Direction
- highe0
- Qualifier
- 47 o Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The flowers collected in June, the hottest month (47 o C) of 2021, exhibited a high expression of PcHsp18.2.”
t0“Prosopis cineraria”
Claim · model-written summary
Collected pollen germination was 80%.
- Taxon
- Prosopis cinerariaProsopis cinerariat0
- Organ / stage
- pollene0
- Outcome
- germinatione0
- Value
- 80%e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The germination of the pollen collected was 80%, and the trees eventually set seeds.”
t0“Prosopis cineraria”
Claim · model-written summary
The trees eventually set seeds.
- Taxon
- Prosopis cinerariaProsopis cinerariat0
- Outcome
- set seedse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The germination of the pollen collected was 80%, and the trees eventually set seeds.”
t0“Prosopis cineraria”
Claim · model-written summary
The PcHsp18.2 promoter and terminator drove highest gusA expression under heat stress in tobacco.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- heat stresse0
- Gene
- gusAe0
- Outcome
- expressione0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Comprehensive analysis of the promoter ( pPcHSP18.2 ) and terminator ( tPchsp18.2 ) of PcHsp18.2 by expressing the gusA in tobacco exhibited the highest expression under heat stress as similar to the expression of PcHsp18.2 in environmental samples.”
Claim · model-written summary
Ectopic gusA expression under PcHsp18.2 regulatory elements increased seed set under heat stress.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- Ectopic expression of gusA under PcHsp18.2 promoter and terminatore0
- Outcome
- seed sete0
- Direction
- increasede0
- Qualifier
- under heat stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of gusA under PcHsp18.2 promoter and terminator resulted in an increased seed set due to the viability of pollen and stigma under heat stress.”
e1“by expressing the gusA in tobacco exhibited the highest expression under heat stress”
Claim · model-written summary
Pollen and stigma viability explained increased seed set under heat stress.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- seed sete0
- Qualifier
- viability of pollen and stigmae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of gusA under PcHsp18.2 promoter and terminator resulted in an increased seed set due to the viability of pollen and stigma under heat stress.”
e1“by expressing the gusA in tobacco exhibited the highest expression under heat stress”
Claim · model-written summary
Anther gusA expression under PcHsp18.2 elements was higher than Lat52 and g10 under heat stress.
- Taxon
- tobaccoNicotiana tabacume1
- Organ / stage
- anthere0
- Treatment
- PcHsp18.2 promoter and terminatore0
- Gene
- gusAe0
- Outcome
- expressione0
- Direction
- high-folde0
- Comparator
- Lat52 and g10e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The gusA expression under PcHsp18.2 promoter and terminator was high-fold in anther compared to the Lat52 and g10 (endogenous genes under its promoter and terminator) genes under heat stress.”
e1“by expressing the gusA in tobacco exhibited the highest expression under heat stress”
Claim · model-written summary
The authors conclude strong balanced inducible regulatory combinations enable resilient transgenic crops.
Verbatim
e0“The expression of genes under strong and balanced endogenous inducible promoter and terminator combinations, as in the desert-growing P. cineraria in transgenic plants, enables the development of resilient crops.”
The same questions in other papers
overexpression · yield · Nicotiana tabacum (tobacco)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
1 paper more in this group
See all 5 papers →heat · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
4 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- no effect
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.