Abstract
• Original comprehensive report on one-step in vitro mass production of banana propagules. • Thidiazuron outperformed N 6 -benzyladenine, kinetin, meta -Topolin, and zeatin during simultaneous shoot-root development. • Heat map and network plot substantiated the effects of and association among plant growth regulators. • Micromorphological studies on shoot, root and leaf revealed systematic plant growth. • 100% plantlets were acclimatized and clonal fidelity was ensured via cytology-, flow cytometry- and ISSR-based assessment. A unique one-step in vitro propagation protocol via concurrent shoot-root development was established in banana cv. Grand Nain. 6-benzyladenine, kinetin, meta -Topolin, thidiazuron (TDZ), and zeatin (0.5—1.5 mg L − 1 ) individually supplemented in Murashige and Skoog medium were tested for multiple shoot-root regeneration from shoot tip explants. The earliest fresh shoot (∼7 days) and root (∼5 days) initiation, highest number of shoots (∼21) and roots (∼16) with maximum root elongation (16.5 cm) were recorded in 0.5 mg L − 1 TDZ. For all intents and purposes, TDZ (0.5 mg L − 1 ) was recorded to be the optimum formulation in terms of the highest simultaneous shoot and root formation that was corroborated further by matrix plot analysis. On the other hand, network plot analysis and principal component analysis illustrated the association between plant growth regulators based on their effect on growth attributes under study. Histological studies on shoots, roots, and leaves revealed a consistent growth and development of plantlets under in vitro condition. Cent percent plantlets were survived during acclimatization, and performed best in cocopeat in comparison to other substrata (vermiculite, perlite, vermicompost-soil, soil-sand, and sand only). The cytology-, flow cytometry- and ISSR primer-assisted fidelity assessments of the in vitro- regenerants exhibited numerical chromosomal as well as ploidy stability and monomorphic banding patterns without any polymorphism within the in vitro -regeneranted plantlets and with their mother plant. Based on the comprehensive information developed in this study, this protocol should be of immense importance to facilitate single-step large-scale production of true-to-type planting materials and to explore the possible comparative physico-chemical roles of the plant growth regulators used in this study.
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Claims 13
Claim · model-written summary
Thidiazuron outperformed N6-benzyladenine, kinetin, meta-Topolin and zeatin in simultaneous shoot-root development.
Verbatim
e1“established in banana cv. Grand Nain”
e2“Thidiazuron outperformed N 6 -benzyladenine, kinetin, meta -Topolin, and zeatin during simultaneous shoot-root development”
Claim · model-written summary
The earliest fresh shoot initiation occurred at about 7 days in 0.5 mg/L TDZ.
Not reportedComparator
Verbatim
e1“established in banana cv. Grand Nain”
e3“The earliest fresh shoot (∼7 days) and root (∼5 days) initiation, highest number of shoots (∼21) and roots (∼16) with maximum root elongation (16.5 cm) were recorded in 0.5 mg L − 1 TDZ”
Claim · model-written summary
The earliest root initiation occurred at about 5 days in 0.5 mg/L TDZ.
Not reportedComparator
Verbatim
e1“established in banana cv. Grand Nain”
e3“The earliest fresh shoot (∼7 days) and root (∼5 days) initiation, highest number of shoots (∼21) and roots (∼16) with maximum root elongation (16.5 cm) were recorded in 0.5 mg L − 1 TDZ”
Claim · model-written summary
The highest number of shoots (about 21) was recorded in 0.5 mg/L TDZ.
Not reportedComparator
Verbatim
e1“established in banana cv. Grand Nain”
e3“The earliest fresh shoot (∼7 days) and root (∼5 days) initiation, highest number of shoots (∼21) and roots (∼16) with maximum root elongation (16.5 cm) were recorded in 0.5 mg L − 1 TDZ”
Claim · model-written summary
The highest number of roots (about 16) was recorded in 0.5 mg/L TDZ.
Not reportedComparator
Verbatim
e1“established in banana cv. Grand Nain”
e3“The earliest fresh shoot (∼7 days) and root (∼5 days) initiation, highest number of shoots (∼21) and roots (∼16) with maximum root elongation (16.5 cm) were recorded in 0.5 mg L − 1 TDZ”
Claim · model-written summary
Maximum root elongation (16.5 cm) was recorded in 0.5 mg/L TDZ.
Not reportedComparator
Verbatim
e1“established in banana cv. Grand Nain”
e3“The earliest fresh shoot (∼7 days) and root (∼5 days) initiation, highest number of shoots (∼21) and roots (∼16) with maximum root elongation (16.5 cm) were recorded in 0.5 mg L − 1 TDZ”
Claim · model-written summary
Network and PCA analyses showed associations among plant growth regulators based on their effects on growth attributes.
Verbatim
e1“established in banana cv. Grand Nain”
e4“network plot analysis and principal component analysis illustrated the association between plant growth regulators based on their effect on growth attributes under study”
Claim · model-written summary
Regenerated shoots, roots and leaves showed consistent growth and development in vitro.
Verbatim
e1“established in banana cv. Grand Nain”
e5“Histological studies on shoots, roots, and leaves revealed a consistent growth and development of plantlets under in vitro condition”
Claim · model-written summary
All plantlets survived acclimatization.
Verbatim
e1“established in banana cv. Grand Nain”
e6“Cent percent plantlets were survived during acclimatization, and performed best in cocopeat in comparison to other substrata (vermiculite, perlite, vermicompost-soil, soil-sand, and sand only)”
Claim · model-written summary
Cocopeat gave the best acclimatization performance compared with other substrata.
Verbatim
e1“established in banana cv. Grand Nain”
e6“Cent percent plantlets were survived during acclimatization, and performed best in cocopeat in comparison to other substrata (vermiculite, perlite, vermicompost-soil, soil-sand, and sand only)”
Claim · model-written summary
In vitro regenerants showed numerical chromosomal and ploidy stability relative to the mother plant.
Verbatim
e1“established in banana cv. Grand Nain”
e7“exhibited numerical chromosomal as well as ploidy stability and monomorphic banding patterns without any polymorphism within the in vitro -regeneranted plantlets and with their mother plant”
Claim · model-written summary
In vitro regenerants showed monomorphic banding patterns without polymorphism relative to the mother plant.
Verbatim
e1“established in banana cv. Grand Nain”
e7“exhibited numerical chromosomal as well as ploidy stability and monomorphic banding patterns without any polymorphism within the in vitro -regeneranted plantlets and with their mother plant”
Claim · model-written summary
The protocol is recommended for single-step large-scale production of true-to-type planting materials.
Verbatim
e1“established in banana cv. Grand Nain”
e2“this protocol should be of immense importance to facilitate single-step large-scale production of true-to-type planting materials”
The same questions in other papers
Thidiazuron · growth · MusaResults differ
19 papers in the same direction as this paper · 2 in the opposite direction
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See all 22 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.