Paper · 2012 · PubMed

Effects of N(6)-benzylaminopurine and Indole Acetic Acid on In Vitro Shoot Multiplication, Nodule-like Meristem Proliferation and Plant Regeneration of Malaysian Bananas (Musa spp.).

Philip Sipen; Michael R Davey

7
claims
12
verbatim quotes
13
citations
2
research areas
✓
Open access

Abstract

Different concentrations of N(6)-benzylaminopurine (BAP) and indole acetic acid (IAA) in Murashige and Skoog based medium were assessed for their effects on shoot multiplication, nodule-like meristem proliferation and plant regeneration of the Malaysian banana cultivars Pisang Mas, Pisang Nangka, Pisang Berangan and Pisang Awak. BAP at 1-14 mg L(-1) with or without 0.2 mg L(-1) IAA, or BAP at 7-14 mg L(-1) with the same concentration of IAA, was evaluated for shoot multiplication from shoot tips and the proliferation of nodule-like meristems from scalps, respectively. Plant regeneration from scalps was assessed using 1 mg L(-1) BAP and 0.2 mg L(-1) IAA separately, or a combination of these two growth regulators. Data on shoot multiplication, the proliferation of nodule-like meristems with associated plant regeneration were recorded after 30 days of culture. A maximum of 5 shoots per original shoot tip was achieved on medium supplemented with BAP at 5 mg L(-1) (Pisang Nangka), 6 mg L(-1) (Pisang Mas and Pisang Berangan), or 7 mg L(-1) (Pisang Awak), with 0.2 mg L(-1) IAA. BAP at 11 mg L(-1) with 0.2 mg L(-1) IAA induced the most highly proliferating nodule-like meristems in the four banana cultivars. Plant regeneration from scalps was optimum in all cases on medium containing 1 mg L(-1) BAP and 0.2 mg L(-1) IAA. This is the first report on the successful induction of highly proliferating nodule-like meristems and plant regeneration from scalps of the Malaysian banana cultivars Pisang Mas, Pisang Nangka, Pisang Berangan and Pisang Awak.

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Claims 7

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cultivar Pisang Nangka reached five shoots per shoot tip with BAP and IAA in culture.

Taxon
Bananast0
Material
Pisang Nangkae0
Organ / stage
shoot tipe0
Treatment
BAP at 5 mg L(-1)e0
Outcome
shoots per original shoot tipe0
Direction
maximume0
Value
5 shoots per original shoot tipe0

Not reportedComparator

Verbatim

e0

“A maximum of 5 shoots per original shoot tip was achieved on medium supplemented with BAP at 5 mg L(-1) (Pisang Nangka), 6 mg L(-1) (Pisang Mas and Pisang Berangan), or 7 mg L(-1) (Pisang Awak), with 0.2 mg L(-1) IAA.”

✓ located verbatim in the source · abstract, characters 870–1087 · show in text ↑
t0

“Bananas”

✓ located verbatim in the source · title, characters 162–169 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cultivar Pisang Mas reached five shoots per shoot tip with BAP and IAA in culture.

Taxon
Bananast0
Material
Pisang Mase0
Organ / stage
shoot tipe0
Treatment
6 mg L(-1)e0
Outcome
shoots per original shoot tipe0
Direction
maximume0
Value
5 shoots per original shoot tipe0

Not reportedComparator

Verbatim

e0

“A maximum of 5 shoots per original shoot tip was achieved on medium supplemented with BAP at 5 mg L(-1) (Pisang Nangka), 6 mg L(-1) (Pisang Mas and Pisang Berangan), or 7 mg L(-1) (Pisang Awak), with 0.2 mg L(-1) IAA.”

✓ located verbatim in the source · abstract, characters 870–1087 · show in text ↑
t0

“Bananas”

✓ located verbatim in the source · title, characters 162–169 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cultivar Pisang Berangan reached five shoots per shoot tip with BAP and IAA in culture.

Taxon
Bananast0
Material
Pisang Berangane0
Organ / stage
shoot tipe0
Treatment
6 mg L(-1)e0
Outcome
shoots per original shoot tipe0
Direction
maximume0
Value
5 shoots per original shoot tipe0

Not reportedComparator

Verbatim

e0

“A maximum of 5 shoots per original shoot tip was achieved on medium supplemented with BAP at 5 mg L(-1) (Pisang Nangka), 6 mg L(-1) (Pisang Mas and Pisang Berangan), or 7 mg L(-1) (Pisang Awak), with 0.2 mg L(-1) IAA.”

✓ located verbatim in the source · abstract, characters 870–1087 · show in text ↑
t0

“Bananas”

✓ located verbatim in the source · title, characters 162–169 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cultivar Pisang Awak reached five shoots per shoot tip with BAP and IAA in culture.

Taxon
Bananast0
Material
Pisang Awake0
Organ / stage
shoot tipe0
Treatment
7 mg L(-1)e0
Outcome
shoots per original shoot tipe0
Direction
maximume0
Value
5 shoots per original shoot tipe0

Not reportedComparator

Verbatim

e0

“A maximum of 5 shoots per original shoot tip was achieved on medium supplemented with BAP at 5 mg L(-1) (Pisang Nangka), 6 mg L(-1) (Pisang Mas and Pisang Berangan), or 7 mg L(-1) (Pisang Awak), with 0.2 mg L(-1) IAA.”

✓ located verbatim in the source · abstract, characters 870–1087 · show in text ↑
t0

“Bananas”

✓ located verbatim in the source · title, characters 162–169 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

BAP at 11 mg L(-1) with IAA induced the most proliferating nodule like meristems in four banana cultivars.

Taxon
bananae0
Material
four banana cultivarse0
Treatment
BAP at 11 mg L(-1) with 0.2 mg L(-1) IAAe0
Outcome
nodule-like meristemse0
Direction
most highly proliferatinge0

Not reportedComparator

Verbatim

e0

“BAP at 11 mg L(-1) with 0.2 mg L(-1) IAA induced the most highly proliferating nodule-like meristems in the four banana cultivars.”

✓ located verbatim in the source · abstract, characters 1088–1218 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Regeneration from scalps was optimal on 1 mg L(-1) BAP with 0.2 mg L(-1) IAA.

Taxon
bananat0
Organ / stage
scalpse0
Treatment
1 mg L(-1) BAP and 0.2 mg L(-1) IAAe0
Outcome
Plant regeneration from scalpse0
Direction
optimume0

Not reportedComparator

Verbatim

e0

“Plant regeneration from scalps was optimum in all cases on medium containing 1 mg L(-1) BAP and 0.2 mg L(-1) IAA.”

✓ located verbatim in the source · abstract, characters 1219–1332 · show in text ↑
t0

“bananas”

✓ located after whitespace/quote normalisation · title, characters 162–169 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

The study reports successful induction of proliferating meristems and regeneration from scalps in four Malaysian banana cultivars.

Taxon
bananae0
Outcome
successful induction of highly proliferating nodule-like meristems and plant regeneration from scalpse0

Verbatim

e0

“This is the first report on the successful induction of highly proliferating nodule-like meristems and plant regeneration from scalps of the Malaysian banana cultivars Pisang Mas, Pisang Nangka, Pisang Berangan and Pisang Awak.”

✓ located verbatim in the source · abstract, characters 1333–1560 · show in text ↑

N-benzyladenine · growth · MusaResults differ

57 papers: 51 positive, 6 negative or no effect

50 papers in the same direction as this paper · 5 in the opposite direction · 1 reporting no difference from an alternative

  1. no effect
  2. negative
  3. positive

53 papers more in this group

See all 57 papers →

indole-3-acetic acid · growth · MusaPositive and negative results

23 papers: 21 positive, 2 negative or no effect

20 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. positive
  3. positive

19 papers more in this group

See all 23 papers →

indole-3-acetic acid · symbiosis · MusaResults agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

N-benzyladenine · symbiosis · MusaResults agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.