Abstract
Hundreds of proteins involved in signaling pathways contain a Ca(2+)-dependent membrane-binding motif called the C2-domain. However, no small C2-domain proteins consisting of a single C2-domain have been reported in animal cells. We have isolated two cDNA clones, OsERG1a and OsERG1b, that encode two small C2-domain proteins of 156 and 159 amino acids, respectively, from a fungal elicitor-treated rice cDNA library. The clones are believed to have originated from a single gene by alternative splicing. Transcript levels of the OsERG1 gene are dramatically elevated by a fungal elicitor prepared from Magnaporthe grisea or by Ca(2+) ions. The OsERG1 protein produced in Escherichia coli binds to phospholipid vesicles in a Ca(2+)-dependent manner and is translocated to the plasma membrane of plant cells by treatment with either a fungal elicitor or a Ca(2+) ionophore. These results suggest that OsERG1 proteins containing a single C2-domain are involved in plant defense signaling systems.
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Claims 7
Claim · model-written summary
Two rice cDNA clones encode small C2-domain proteins of 156 and 159 amino acids.
- Taxon
- rice
Oryza sativae0 - Gene
- OsERG1a and OsERG1be0
- Outcome
- two small C2-domain proteinse0
- Value
- 156 and 159 amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have isolated two cDNA clones, OsERG1a and OsERG1b, that encode two small C2-domain proteins of 156 and 159 amino acids, respectively, from a fungal elicitor-treated rice cDNA library”
Claim · model-written summary
The clones are believed to originate from one gene by alternative splicing.
- Taxon
- RiceOryza sativat0
- Gene
- OsERG1a and OsERG1be1
- Outcome
- originated from a single genee0
- Qualifier
- alternative splicinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The clones are believed to have originated from a single gene by alternative splicing”
e1“OsERG1a and OsERG1b”
t0“Rice”
Claim · model-written summary
A Magnaporthe grisea fungal elicitor dramatically elevated OsERG1 transcript levels.
- Taxon
- RiceOryza sativat0
- Treatment
- fungal elicitor prepared from Magnaporthe griseae0
- Interactor
- Magnaporthe grisea
Pyricularia griseae0pathogen - Gene
- OsERG1e0
- Outcome
- Transcript levelse0
- Direction
- dramatically elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcript levels of the OsERG1 gene are dramatically elevated by a fungal elicitor prepared from Magnaporthe grisea”
t0“Rice”
Claim · model-written summary
Calcium ions dramatically elevated OsERG1 transcript levels.
- Taxon
- RiceOryza sativat0
- Treatment
- Ca(2+) ionse0
- Gene
- OsERG1e0
- Outcome
- Transcript levelse0
- Direction
- dramatically elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcript levels of the OsERG1 gene are dramatically elevated by a fungal elicitor prepared from Magnaporthe grisea or by Ca(2+) ions”
t0“Rice”
Claim · model-written summary
OsERG1 protein bound phospholipid vesicles in a calcium-dependent manner.
- Taxon
- RiceOryza sativat0
- Gene
- OsERG1 proteine0
- Outcome
- binds to phospholipid vesiclese0
- Qualifier
- Ca(2+)-dependent mannere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The OsERG1 protein produced in Escherichia coli binds to phospholipid vesicles in a Ca(2+)-dependent manner”
t0“Rice”
Claim · model-written summary
Fungal elicitor treatment translocated OsERG1 protein to the plant cell plasma membrane.
- Taxon
- RiceOryza sativat0
- Treatment
- a fungal elicitore0
- Gene
- OsERG1 proteine1
- Outcome
- translocated to the plasma membrane of plant cellse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“is translocated to the plasma membrane of plant cells by treatment with either a fungal elicitor or a Ca(2+) ionophore”
e1“The OsERG1 protein”
t0“Rice”
Claim · model-written summary
Calcium ionophore treatment translocated OsERG1 protein to the plant cell plasma membrane.
- Taxon
- RiceOryza sativat0
- Treatment
- Ca(2+) ionophoree0
- Gene
- OsERG1 proteine1
- Outcome
- translocated to the plasma membrane of plant cellse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“is translocated to the plasma membrane of plant cells by treatment with either a fungal elicitor or a Ca(2+) ionophore”
e1“The OsERG1 protein”
t0“Rice”
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See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.