Abstract
Linolenic acid (18:3) is the most abundant fatty acid in plant membrane lipids and is a source for various oxidized metabolites, called oxylipins. 18:3 and oxylipins play important roles in the induction of defense responses to pathogen infection and wound stress in Arabidopsis. However, in rice, endogenous roles for 18:3 and oxylipins in disease resistance have not been confirmed. We generated 18:3-deficient transgenic rice plants (F78Ri) with co-suppression of two omega-3 fatty acid desaturases, OsFAD7 and OsFAD8. that synthesize 18:3. The F78Ri plants showed enhanced resistance to the phytopathogenic fungus Magnaporthe grisea. A typical 18:3-derived oxylipin, jasmonic acid (JA), acts as a signaling molecule in defense responses to fungal infection in Arabidopsis. However, in F78Ri plants, the expression of JA-responsive pathogenesis-related genes, PBZ1 and PR1b, was induced after inoculation with M. grisea, although the JA-mediated wound response was suppressed. Furthermore, the application of JA methyl ester had no significant effect on the enhanced resistance in F78Ri plants. Taken together, our results indicate that, although suppression of fatty acid desaturases involves the concerted action of varied oxylipins via diverse metabolic pathways, 18:3 or 18:3-derived oxylipins, except for JA, may contribute to signaling on defense responses of rice to M. grisea infection.
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Claims 5
Claim · model-written summary
Transgenic rice F78Ri with co-suppressed OsFAD7 and OsFAD8 showed enhanced resistance to Magnaporthe grisea.
- Taxon
- rice
Oryza sativae1 - Material
- F78Rie2
- Treatment
- co-suppression of two omega-3 fatty acid desaturasese1
- Interactor
- Magnaporthe grisea
Pyricularia griseae2pathogen - Gene
- OsFAD7 and OsFAD8e1
- Outcome
- resistancee2
- Direction
- enhancede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We generated 18:3-deficient transgenic rice plants (F78Ri) with co-suppression of two omega-3 fatty acid desaturases, OsFAD7 and OsFAD8”
e2“The F78Ri plants showed enhanced resistance to the phytopathogenic fungus Magnaporthe grisea”
Claim · model-written summary
After inoculation with M. grisea, JA-responsive pathogenesis-related genes PBZ1 and PR1b were induced in F78Ri rice.
- Taxon
- riceOryza sativae1
- Material
- F78Rie3
- Treatment
- inoculation with M. griseae3
- Interactor
- M. griseaPyricularia griseae3pathogen
- Gene
- PBZ1 and PR1be3
- Outcome
- expression of JA-responsive pathogenesis-related genese3
- Direction
- inducede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We generated 18:3-deficient transgenic rice plants (F78Ri) with co-suppression of two omega-3 fatty acid desaturases, OsFAD7 and OsFAD8”
e3“However, in F78Ri plants, the expression of JA-responsive pathogenesis-related genes, PBZ1 and PR1b, was induced after inoculation with M. grisea”
Claim · model-written summary
The JA-mediated wound response was suppressed in F78Ri rice.
- Taxon
- riceOryza sativae1
- Material
- F78Rie3
- Outcome
- JA-mediated wound responsee6
- Direction
- suppressede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We generated 18:3-deficient transgenic rice plants (F78Ri) with co-suppression of two omega-3 fatty acid desaturases, OsFAD7 and OsFAD8”
e3“However, in F78Ri plants, the expression of JA-responsive pathogenesis-related genes, PBZ1 and PR1b, was induced after inoculation with M. grisea”
e6“although the JA-mediated wound response was suppressed”
Claim · model-written summary
Applying JA methyl ester had no significant effect on the enhanced resistance of F78Ri rice.
- Taxon
- riceOryza sativae1
- Material
- F78Rie4
- Treatment
- application of JA methyl estere4
- Outcome
- resistancee4
- Direction
- no significant effecte4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We generated 18:3-deficient transgenic rice plants (F78Ri) with co-suppression of two omega-3 fatty acid desaturases, OsFAD7 and OsFAD8”
e4“Furthermore, the application of JA methyl ester had no significant effect on the enhanced resistance in F78Ri plants”
Claim · model-written summary
Oxylipins other than JA derived from 18:3 may contribute to signalling in rice defense against M. grisea.
- Taxon
- riceOryza sativae5
- Interactor
- M. griseaPyricularia griseae5pathogen
- Compound
- 18:3-derived oxylipinse5
- Outcome
- defense responsese5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“18:3 or 18:3-derived oxylipins, except for JA, may contribute to signaling on defense responses of rice to M. grisea infection”
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