Abstract
OsRac1, one of the Rac/Rop family of small GTPases, plays important roles in defense responses, including a role in the production of reactive oxygen species mediated by NADPH oxidase. We have identified an effector of OsRac1, namely rice (Oryza sativa) cinnamoyl-CoA reductase 1 (OsCCR1), an enzyme involved in lignin biosynthesis. Lignin, which is polymerized through peroxidase activity by using H(2)O(2) in the cell wall, is an important factor in plant defense responses, because it presents an undegradable mechanical barrier to most pathogens. Expression of OsCCR1 was induced by a sphingolipid elicitor, suggesting that OsCCR1 participates in defense signaling. In in vitro interaction and two-hybrid experiments, OsRac1 was shown to bind OsCCR1 in a GTP-dependent manner. Moreover, the interaction of OsCCR1 with OsRac1 led to the enzymatic activation of OsCCR1 in vitro. Transgenic cell cultures expressing the constitutively active OsRac1 accumulated lignin through enhanced CCR activity and increased reactive oxygen species production. Thus, it is likely that OsRac1 controls lignin synthesis through regulation of both NADPH oxidase and OsCCR1 activities during defense responses in rice.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Rice OsCCR1 was identified as an OsRac1 effector.
- Taxon
- rice
Oryza sativae0 - Gene
- cinnamoyl-CoA reductase 1 (OsCCR1)e0
- Outcome
- an effector of OsRac1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have identified an effector of OsRac1, namely rice (Oryza sativa) cinnamoyl-CoA reductase 1 (OsCCR1)”
Claim · model-written summary
A sphingolipid elicitor induced OsCCR1 expression.
- Taxon
- riceOryza sativae1
- Treatment
- a sphingolipid elicitore0
- Gene
- OsCCR1e0
- Outcome
- Expression of OsCCR1e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of OsCCR1 was induced by a sphingolipid elicitor”
e1“rice (Oryza sativa)”
Claim · model-written summary
OsRac1 bound OsCCR1 in a GTP-dependent manner in vitro and in two-hybrid experiments.
- Taxon
- riceOryza sativae1
- Gene
- OsRac1e0
- Outcome
- bind OsCCR1 in a GTP-dependent mannere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In in vitro interaction and two-hybrid experiments, OsRac1 was shown to bind OsCCR1 in a GTP-dependent manner”
e1“rice (Oryza sativa)”
Claim · model-written summary
OsRac1 interaction activated OsCCR1 enzymatic activity in vitro.
- Taxon
- riceOryza sativae1
- Gene
- OsCCR1e0
- Outcome
- enzymatic activation of OsCCR1e0
- Qualifier
- interaction of OsCCR1 with OsRac1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the interaction of OsCCR1 with OsRac1 led to the enzymatic activation of OsCCR1 in vitro”
e1“rice (Oryza sativa)”
Claim · model-written summary
Cells expressing constitutively active OsRac1 accumulated lignin through enhanced CCR activity.
- Taxon
- riceOryza sativae1
- Treatment
- expressing the constitutively active OsRac1e0
- Gene
- OsRac1e0
- Outcome
- accumulated lignine0
- Direction
- accumulatede0
- Qualifier
- through enhanced CCR activitye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic cell cultures expressing the constitutively active OsRac1 accumulated lignin through enhanced CCR activity and increased reactive oxygen species production”
e1“rice (Oryza sativa)”
Claim · model-written summary
Constitutively active OsRac1 increased CCR activity in rice cell cultures.
- Taxon
- riceOryza sativae1
- Treatment
- expressing the constitutively active OsRac1e0
- Gene
- OsRac1e0
- Outcome
- CCR activitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic cell cultures expressing the constitutively active OsRac1 accumulated lignin through enhanced CCR activity and increased reactive oxygen species production”
e1“rice (Oryza sativa)”
Claim · model-written summary
Constitutively active OsRac1 increased reactive oxygen species production in rice cell cultures.
- Taxon
- riceOryza sativae1
- Treatment
- expressing the constitutively active OsRac1e0
- Gene
- OsRac1e0
- Outcome
- reactive oxygen species productione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic cell cultures expressing the constitutively active OsRac1 accumulated lignin through enhanced CCR activity and increased reactive oxygen species production”
e1“rice (Oryza sativa)”
Claim · model-written summary
OsRac1 likely controls lignin synthesis through NADPH oxidase and OsCCR1 activity in rice defense.
- Taxon
- riceOryza sativae0
- Gene
- OsRac1e0
- Outcome
- lignin synthesise0
- Qualifier
- through regulation of both NADPH oxidase and OsCCR1 activitiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it is likely that OsRac1 controls lignin synthesis through regulation of both NADPH oxidase and OsCCR1 activities during defense responses in rice”
The same questions in other papers
resistance inducer · molecular: expression · Oryza sativa (rice)Positive and negative results
14 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
13 papers more in this group
See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.