Abstract
An experiment on virus free in vitro banana plantlets production by the meristem cultured from virus infected four month old banana suckers cv. Amritasagar were used as explants. Four levels of BAP (0, 3, 4 and 5 mg/l), 5 levels of NAA (0.0, 1.0, 1.5, 2.0 and 2.5 mg/l) were used as treatment for shoot proliferation in experiment-I and Four levels of IBA (0, 1, 2 and 3 mg/l), 4 levels of NAA (0, 2, 3 and 4 mg/l) were used for root formation in experiment-II. ELISA test was conducted for the confirmation of virus eradication rate of regenerated plants and survival rate of regenerated plants in experiment-III. A significant variation was found among the treatments. Studies on the different concentrations of BAP + NAA on shoot proliferation and differentiation, reveals that 4 mg/l BAP + 1.5 mg/l NAA produced the greater number of shoots (4.58/explant), the longest shoot (3.56 cm) and the highest numbers of leaves 3.11/explant). On the contrary, plantlets were cultured on half strength of MS media in different levels of IBA and NAA.The highest number of vigorous roots (6.98/explant) and maximum root length (5 cm) were recorded from the treatment combination 2 mg/l IBA+2 mg/l NAA and 3 mg/l IBA+ 3 mg/l NAA, respectively. While less vigorous root was found in control treatments. The rate of virus free plant regeneration from banana bunchy top virus (BBTV) infected plants was 57.14% and from banana mosaic virus (BMV) infected sources was 64.28%. The highest plant survival rate 83.33% was recorded in a mixture of sand, soil and cow dung (1:1:1). On the other hand, 67.67% plant survival rate was found in a mixture of coir and soil (1:2). Eradication of banana viruses from infected plants by meristem cultured is a useful tactic that will be helpful for commercial exploitation and virus free plant production for use of farmer’s level.
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Claims 10
Claim · model-written summary
BAP plus NAA produced 4.58 shoots per explant.
- Taxon
- bananat0
- Treatment
- 4 mg/l BAP + 1.5 mg/l NAAe0
- Outcome
- number of shootse0
- Direction
- greatere0
- Value
- 4.58/explante0
Not reportedComparator
Verbatim
e0“4 mg/l BAP + 1.5 mg/l NAA produced the greater number of shoots (4.58/explant)”
t0“Banana cv. Amritasagar”
Claim · model-written summary
BAP plus NAA produced the longest shoots at 3.56 cm.
Not reportedComparator
Verbatim
e0“4 mg/l BAP + 1.5 mg/l NAA produced the greater number of shoots (4.58/explant), the longest shoot (3.56 cm)”
t0“Banana cv. Amritasagar”
Claim · model-written summary
BAP plus NAA produced the highest leaf count at 3.11 per explant.
Not reportedTreatmentComparator
Verbatim
e0“the highest numbers of leaves 3.11/explant)”
t0“Banana cv. Amritasagar”
Claim · model-written summary
The 2 mg/l IBA plus 2 mg/l NAA combination yielded 6.98 vigorous roots per explant.
- Taxon
- bananat0
- Treatment
- 2 mg/l IBA+2 mg/l NAAe0
- Outcome
- number of vigorous rootse0
- Direction
- higheste0
- Value
- 6.98/explante0
Not reportedComparator
Verbatim
e0“The highest number of vigorous roots (6.98/explant) and maximum root length (5 cm) were recorded from the treatment combination 2 mg/l IBA+2 mg/l NAA and 3 mg/l IBA+ 3 mg/l NAA, respectively.”
t0“Banana cv. Amritasagar”
Claim · model-written summary
The 3 mg/l IBA plus 3 mg/l NAA combination yielded the longest roots at 5 cm.
Not reportedComparator
Verbatim
e0“maximum root length (5 cm) were recorded from the treatment combination 2 mg/l IBA+2 mg/l NAA and 3 mg/l IBA+ 3 mg/l NAA, respectively.”
t0“Banana cv. Amritasagar”
Claim · model-written summary
Control treatments produced less vigorous roots.
Not reportedComparator
Verbatim
e0“less vigorous root was found in control treatments.”
t0“Banana cv. Amritasagar”
Claim · model-written summary
Virus-free regeneration from BBTV-infected banana was 57.14%.
- Taxon
- bananae0
- Interactor
- banana bunchy top virus (BBTV)
Banana bunchy top viruse0pathogen - Outcome
- rate of virus free plant regeneratione0
- Value
- 57.14%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rate of virus free plant regeneration from banana bunchy top virus (BBTV) infected plants was 57.14%”
Claim · model-written summary
Virus-free regeneration from BMV-infected banana was 64.28%.
- Taxon
- bananat0
- Interactor
- banana mosaic virus (BMV)
Brome mosaic viruse0pathogen - Outcome
- virus free plant regeneratione0
- Value
- 64.28%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rate of virus free plant regeneration from banana bunchy top virus (BBTV) infected plants was 57.14% and from banana mosaic virus (BMV) infected sources was 64.28%”
t0“Banana Mosaic Virus (BMV) from Infected Plant of Banana”
Claim · model-written summary
Plant survival reached 83.33% in sand, soil and cow dung at 1:1:1.
- Taxon
- bananat0
- Treatment
- a mixture of sand, soil and cow dung (1:1:1)e0
- Outcome
- plant survival ratee0
- Direction
- higheste0
- Value
- 83.33%e0
Not reportedComparator
Verbatim
e0“The highest plant survival rate 83.33% was recorded in a mixture of sand, soil and cow dung (1:1:1).”
t0“Banana cv. Amritasagar”
Claim · model-written summary
Plant survival was 67.67% in coir and soil at 1:2.
Verbatim
e0“67.67% plant survival rate was found in a mixture of coir and soil (1:2).”
t0“Banana cv. Amritasagar”
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