Abstract
This study shows that dehydration induces imbalanced metabolism before loss of membrane integrity in desiccation-sensitive germinated radicles. Using a photoacoustic detection system, responses of CO(2) emission and fermentation to drying were analyzed non-invasively in desiccation-tolerant and -intolerant radicles of cucumber (Cucumis sativa) and pea (Pisum sativum). Survival after drying and a membrane integrity assay showed that desiccation tolerance was present during early imbibition and lost in germinated radicles. However, tolerance could be re-induced in germinated cucumber radicles by incubation in polyethylene glycol before drying. Tolerant and polyethylene glycol (PEG)-induced tolerant radicles exhibited a much-reduced CO(2) production before dehydration compared with desiccation-sensitive radicles. This difference was maintained during dehydration. In desiccation-sensitive tissues, dehydration induced an increase in the emission of acetaldehyde and ethanol that peaked well before the loss of membrane integrity. Acetaldehyde emission from sensitive radicles was significantly reduced when dehydration occurred in 50% O(2) instead of air. Acetaldehyde/ethanol were not detected in dehydrating tolerant radicles of either species or in polyethylene glycol-induced tolerant cucumber radicles. Thus, a balance between down-regulation of metabolism during drying and O(2) availability appears to be associated with desiccation tolerance. Using Fourier transform infrared spectroscopy, acetaldehyde was found to disturb the phase behavior of phospholipid vesicles, suggesting that the products resulting from imbalanced metabolism in seeds may aggravate membrane damage induced by dehydration.
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Claims 10
Claim · model-written summary
Desiccation tolerance was present during early imbibition and lost in germinated cucumber and pea radicles.
- Taxon
- cucumber (Cucumis sativa) and pea (Pisum sativum)
Cucumis sativus
Lathyrus oleraceuse0 - Organ / stage
- germinated radiclese1
- Outcome
- desiccation tolerancee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“radicles of cucumber (Cucumis sativa) and pea (Pisum sativum)”
e1“desiccation tolerance was present during early imbibition and lost in germinated radicles”
Claim · model-written summary
Polyethylene glycol incubation restored desiccation tolerance in germinated cucumber radicles.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- germinated cucumber radiclese0
- Treatment
- incubation in polyethylene glycole0
- Outcome
- tolerancee0
- Direction
- re-inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tolerance could be re-induced in germinated cucumber radicles by incubation in polyethylene glycol before drying”
Claim · model-written summary
Tolerant and PEG-induced tolerant radicles had lower CO(2) production before dehydration than sensitive radicles, a difference maintained during drying.
- Taxon
- cucumber (Cucumis sativa) and pea (Pisum sativum)Cucumis sativusLathyrus oleraceuse1
- Outcome
- CO(2) productione0
- Direction
- much-reducede0
- Qualifier
- before dehydratione0
- Comparator
- desiccation-sensitive radiclese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tolerant and polyethylene glycol (PEG)-induced tolerant radicles exhibited a much-reduced CO(2) production before dehydration compared with desiccation-sensitive radicles. This difference was maintained during dehydration”
e1“radicles of cucumber (Cucumis sativa) and pea (Pisum sativum)”
Claim · model-written summary
Dehydration increased acetaldehyde emission in desiccation-sensitive radicles before membrane integrity was lost.
- Taxon
- cucumber (Cucumis sativa) and pea (Pisum sativum)Cucumis sativusLathyrus oleraceuse0
- Treatment
- dehydratione1
- Outcome
- emission of acetaldehydee1
- Direction
- increasee1
- Qualifier
- well before the loss of membrane integritye1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“radicles of cucumber (Cucumis sativa) and pea (Pisum sativum)”
e1“dehydration induced an increase in the emission of acetaldehyde and ethanol that peaked well before the loss of membrane integrity”
Claim · model-written summary
Dehydration increased ethanol emission in desiccation-sensitive radicles before membrane integrity was lost.
- Taxon
- cucumber (Cucumis sativa) and pea (Pisum sativum)Cucumis sativusLathyrus oleraceuse0
- Treatment
- dehydratione1
- Outcome
- ethanole1
- Direction
- increasee1
- Qualifier
- well before the loss of membrane integritye1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“radicles of cucumber (Cucumis sativa) and pea (Pisum sativum)”
e1“dehydration induced an increase in the emission of acetaldehyde and ethanol that peaked well before the loss of membrane integrity”
Claim · model-written summary
Drying in 50% oxygen significantly reduced acetaldehyde emission compared with air.
- Taxon
- cucumber (Cucumis sativa) and pea (Pisum sativum)Cucumis sativusLathyrus oleraceuse0
- Treatment
- 50% O(2)e1
- Outcome
- Acetaldehyde emission from sensitive radiclese1
- Direction
- reducede1
- Comparator
- aire1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“radicles of cucumber (Cucumis sativa) and pea (Pisum sativum)”
e1“Acetaldehyde emission from sensitive radicles was significantly reduced when dehydration occurred in 50% O(2) instead of air”
Claim · model-written summary
Acetaldehyde and ethanol were undetected during dehydration of tolerant cucumber and pea radicles.
- Taxon
- cucumber (Cucumis sativa) and pea (Pisum sativum)Cucumis sativusLathyrus oleraceuse1
- Outcome
- Acetaldehyde/ethanole0
- Qualifier
- dehydrating tolerant radiclese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Acetaldehyde/ethanol were not detected in dehydrating tolerant radicles of either species”
e1“radicles of cucumber (Cucumis sativa) and pea (Pisum sativum)”
Claim · model-written summary
Acetaldehyde and ethanol were undetected in PEG-induced tolerant cucumber radicles.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- cucumber radiclese0
- Outcome
- Acetaldehyde/ethanole0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Acetaldehyde/ethanol were not detected in dehydrating tolerant radicles of either species or in polyethylene glycol-induced tolerant cucumber radicles”
Claim · model-written summary
Acetaldehyde disturbed phospholipid vesicle phase behavior in spectroscopy assays.
- Taxon
- cucumber (Cucumis sativa) and pea (Pisum sativum)Cucumis sativusLathyrus oleraceuse0
- Compound
- acetaldehydee1
- Outcome
- phase behavior of phospholipid vesiclese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“radicles of cucumber (Cucumis sativa) and pea (Pisum sativum)”
e1“acetaldehyde was found to disturb the phase behavior of phospholipid vesicles”
Claim · model-written summary
The authors associated desiccation tolerance with balanced metabolic downregulation and oxygen availability during drying.
- Taxon
- cucumber (Cucumis sativa) and pea (Pisum sativum)Cucumis sativusLathyrus oleraceuse0
- Compound
- O(2)e1
- Outcome
- desiccation tolerancee1
- Qualifier
- down-regulation of metabolism during dryinge1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“radicles of cucumber (Cucumis sativa) and pea (Pisum sativum)”
e1“a balance between down-regulation of metabolism during drying and O(2) availability appears to be associated with desiccation tolerance”
The same questions in other papers
poly(ethylene glycol) · disease · Cucumis sativus (cucumber)Results agree
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- positive
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drought · molecular: metabolites · Cucumis sativus (cucumber)Positive and negative results
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11 papers in the same direction as this paper · 3 in the opposite direction
- positive
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See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.