Abstract
This study establishes a relationship between desiccation tolerance and the transfer of amphiphilic molecules from the cytoplasm into lipids during drying, using electron paramagnetic resonance spectroscopy of amphiphilic spin probes introduced into imbibed radicles of pea (Pisum sativum) and cucumber (Cucumis sativa) seeds. Survival following drying and a membrane integrity assay indicated that desiccation tolerance was present during early imbibition and lost in germinated radicles. In germinated cucumber radicles, desiccation tolerance could be re-induced by an incubation in polyethylene glycol (PEG) before drying. In desiccation-intolerant radicles, partitioning of spin probes into lipids during dehydration occurred at higher water contents compared with tolerant and PEG-induced tolerant radicles. The difference in partitioning behavior between desiccation-tolerant and -intolerant tissues could not be explained by the loss of water. Consequently, using a two-phase model system composed of sunflower or cucumber oil and water, physical properties of the aqueous solvent that may affect the partitioning of amphiphilic spin probes were investigated. A significant relationship was found between the partitioning of spin probes and the viscosity of the aqueous solvent. Moreover, in desiccation-sensitive radicles, the rise in cellular microviscosity during drying commenced at higher water contents compared with tolerant or PEG-induced tolerant radicles, suggesting that the microviscosity of the cytoplasm may control the partitioning behavior in dehydrating seeds.
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Claims 7
Claim · model-written summary
Desiccation tolerance was present during early imbibition and lost in germinated radicles.
Not reportedTaxon
Verbatim
e0“desiccation tolerance was present during early imbibition and lost in germinated radicles.”
Claim · model-written summary
PEG incubation restored desiccation tolerance in germinated cucumber radicles.
- Taxon
- cucumber
Cucumis sativuse0 - Organ / stage
- radiclese0
- Treatment
- polyethylene glycol (PEG)e0
- Outcome
- desiccation tolerancee0
- Direction
- re-inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In germinated cucumber radicles, desiccation tolerance could be re-induced by an incubation in polyethylene glycol (PEG) before drying.”
Claim · model-written summary
Spin probes partitioned into lipids at higher water content in intolerant than tolerant radicles.
- Organ / stage
- radiclese0
- Outcome
- partitioning of spin probes into lipidse0
- Direction
- highere0
- Comparator
- tolerant and PEG-induced tolerant radiclese0
Not reportedTaxon
Verbatim
e0“In desiccation-intolerant radicles, partitioning of spin probes into lipids during dehydration occurred at higher water contents compared with tolerant and PEG-induced tolerant radicles.”
Claim · model-written summary
Water loss did not explain the partitioning difference.
- Outcome
- difference in partitioning behaviore0
Not reportedTaxon
Verbatim
e0“The difference in partitioning behavior between desiccation-tolerant and -intolerant tissues could not be explained by the loss of water.”
Claim · model-written summary
Spin probe partitioning was significantly related to aqueous solvent viscosity.
Not reportedTaxonDirection
Verbatim
e0“A significant relationship was found between the partitioning of spin probes and the viscosity of the aqueous solvent.”
Claim · model-written summary
Cellular microviscosity rose at higher water content in sensitive than tolerant radicles.
- Organ / stage
- radiclese0
- Outcome
- rise in cellular microviscositye0
- Direction
- highere0
- Comparator
- tolerant or PEG-induced tolerant radiclese0
Not reportedTaxon
Verbatim
e0“in desiccation-sensitive radicles, the rise in cellular microviscosity during drying commenced at higher water contents compared with tolerant or PEG-induced tolerant radicles”
Claim · model-written summary
The authors suggest cytoplasmic microviscosity may control partitioning in dehydrating seeds.
Verbatim
e0“the microviscosity of the cytoplasm may control the partitioning behavior in dehydrating seeds.”
The same questions in other papers
poly(ethylene glycol) · disease · Cucumis sativus (cucumber)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.