Abstract
Cotyledons detached from 4-d-old cucumber (Cucumis sativus L.) seedlings were subjected to water stress (air-drying or PEG-treatment) to examine the effects of the stress on carbohydrate metabolism. Amylolytic activity in the cotyledon was increased about 6-fold by water stress within 1 d. The substrate specificity and the action pattern indicated that beta-amylase is responsible for the activity. Activities of azocaseinase, malate dehydrogenase and triose-phosphate isomerase were not affected by water stress, indicating that the effect of the stress on beta-amylase is rather specific. Cycloheximide-treatment strongly reduced the enhancement of beta-amylase activity. The hypocotyl of cucumber seedlings also exhibited an increase in the enzyme activity when subjected to water stress. The major free sugars in cucumber cotyledons were glucose, fructose, maltose, and sucrose; sucrose being the most abundant. Sucrose content in excised, unstressed cotyledons increased markedly during the incubation. Changes in other free sugars were small compared with that of sucrose. Starch also accumulated in unstressed cotyledons. In stressed cotyledons more sucrose and less starch accumulated than in unstressed ones. Such results were discussed in relation to the enhancement of beta-amylase activity.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 13
Claim · model-written summary
Water stress increased cotyledon amylolytic activity about sixfold within one day.
- Taxon
- cucumber
Cucumis sativust0 - Organ / stage
- cotyledone0
- Treatment
- water stresse0
- Outcome
- Amylolytic activitye0
- Direction
- increasede0
- Value
- about 6-folde0
- Qualifier
- within 1 de0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Amylolytic activity in the cotyledon was increased about 6-fold by water stress within 1 d.”
t0“cucumber”
Claim · model-written summary
Substrate specificity and action pattern indicated beta-amylase was responsible for the activity.
- Taxon
- cucumberCucumis sativust0
- Gene
- beta-amylasee0
- Outcome
- activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The substrate specificity and the action pattern indicated that beta-amylase is responsible for the activity.”
t0“cucumber”
Claim · model-written summary
Water stress did not affect azocaseinase, malate dehydrogenase or triose-phosphate isomerase activities.
- Taxon
- cucumberCucumis sativust0
- Treatment
- water stresse0
- Outcome
- Activities of azocaseinase, malate dehydrogenase and triose-phosphate isomerasee0
- Direction
- not affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Activities of azocaseinase, malate dehydrogenase and triose-phosphate isomerase were not affected by water stress”
t0“cucumber”
Claim · model-written summary
The authors interpret the water-stress effect on beta-amylase as rather specific.
- Taxon
- cucumberCucumis sativust0
- Gene
- beta-amylasee0
- Outcome
- rather specifice0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“indicating that the effect of the stress on beta-amylase is rather specific.”
t0“cucumber”
Claim · model-written summary
Cycloheximide treatment strongly reduced enhancement of beta-amylase activity.
- Taxon
- cucumberCucumis sativust0
- Treatment
- Cycloheximide-treatmente0
- Gene
- beta-amylasee0
- Outcome
- enhancement of beta-amylase activitye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cycloheximide-treatment strongly reduced the enhancement of beta-amylase activity.”
t0“cucumber”
Claim · model-written summary
Water stress also increased enzyme activity in cucumber hypocotyls.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- hypocotyle0
- Treatment
- water stresse0
- Outcome
- enzyme activitye0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The hypocotyl of cucumber seedlings also exhibited an increase in the enzyme activity when subjected to water stress.”
Claim · model-written summary
Glucose, fructose and maltose were major free sugars in cucumber cotyledons.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- cotyledonse0
- Outcome
- glucose, fructose, maltosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The major free sugars in cucumber cotyledons were glucose, fructose, maltose, and sucrose; sucrose being the most abundant.”
Claim · model-written summary
Sucrose was the most abundant major free sugar in cucumber cotyledons.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- cotyledonse0
- Outcome
- sucrosee0
- Direction
- most abundante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The major free sugars in cucumber cotyledons were glucose, fructose, maltose, and sucrose; sucrose being the most abundant.”
Claim · model-written summary
Sucrose content markedly increased during incubation of excised unstressed cotyledons.
- Taxon
- cucumberCucumis sativust0
- Organ / stage
- cotyledonse0
- Outcome
- Sucrose contente0
- Direction
- increasede0
- Qualifier
- excised, unstressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sucrose content in excised, unstressed cotyledons increased markedly during the incubation.”
t0“cucumber”
Claim · model-written summary
Changes in other free sugars were small relative to sucrose changes.
- Taxon
- cucumberCucumis sativust0
- Outcome
- Changes in other free sugarse0
- Qualifier
- smalle0
- Comparator
- sucrosee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Changes in other free sugars were small compared with that of sucrose.”
t0“cucumber”
Claim · model-written summary
Starch accumulated in unstressed cotyledons.
- Taxon
- cucumberCucumis sativust0
- Organ / stage
- cotyledonse0
- Outcome
- Starche0
- Direction
- accumulatede0
- Qualifier
- unstressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Starch also accumulated in unstressed cotyledons.”
t0“cucumber”
Claim · model-written summary
Stressed cotyledons accumulated more sucrose than unstressed cotyledons.
- Taxon
- cucumberCucumis sativust0
- Organ / stage
- cotyledonse0
- Treatment
- stressede0
- Outcome
- sucrosee0
- Direction
- moree0
- Comparator
- unstressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In stressed cotyledons more sucrose and less starch accumulated than in unstressed ones.”
t0“cucumber”
Claim · model-written summary
Stressed cotyledons accumulated less starch than unstressed cotyledons.
- Taxon
- cucumberCucumis sativust0
- Organ / stage
- cotyledonse0
- Treatment
- stressede0
- Outcome
- starche0
- Direction
- lesse0
- Comparator
- unstressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In stressed cotyledons more sucrose and less starch accumulated than in unstressed ones.”
t0“cucumber”
The same questions in other papers
drought · molecular: metabolites · Cucumis sativus (cucumber)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- no effect
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.