Abstract
Arabidopsis thaliana ANKYRIN REPEAT-CONTAINING PROTEIN 2A (AKR2A) interacts with peroxisomal membrane-bound ASCORBATE PEROXIDASE3 (APX3). This interaction involves the C-terminal sequence of APX3 (i.e., a transmembrane domain plus a few basic amino acid residues). The specificity of the AKR2A-APX3 interaction suggests that AKR2A may function as a molecular chaperone for APX3 because binding of AKR2A to the transmembrane domain can prevent APX3 from forming aggregates after translation. Analysis of three akr2a mutants indicates that these mutant plants have reduced steady state levels of APX3. Reduced expression of AKR2A using RNA interference also leads to reduced steady state levels of APX3 and reduced targeting of APX3 to peroxisomes in plant cells. Since AKR2A also binds specifically to the chloroplast OUTER ENVELOPE PROTEIN7 (OEP7) and is required for the biogenesis of OEP7, AKR2A may serve as a molecular chaperone for OEP7 as well. The pleiotropic phenotype of akr2a mutants indicates that AKR2A plays many important roles in plant cellular metabolism and is essential for plant growth and development.
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Claims 10
Claim · model-written summary
AKR2A interacts with peroxisomal membrane-bound APX3 in Arabidopsis thaliana.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Gene
- AKR2Ae0
- Outcome
- interacts with peroxisomal membrane-bound ASCORBATE PEROXIDASE3 (APX3)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana ANKYRIN REPEAT-CONTAINING PROTEIN 2A (AKR2A) interacts with peroxisomal membrane-bound ASCORBATE PEROXIDASE3 (APX3)”
Claim · model-written summary
The AKR2A-APX3 interaction involves the C-terminal sequence of APX3.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- APX3e1
- Outcome
- interactione1
- Qualifier
- involves the C-terminal sequence of APX3e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana ANKYRIN REPEAT-CONTAINING PROTEIN 2A (AKR2A) interacts with peroxisomal membrane-bound ASCORBATE PEROXIDASE3 (APX3)”
e1“This interaction involves the C-terminal sequence of APX3 (i.e., a transmembrane domain plus a few basic amino acid residues)”
Claim · model-written summary
AKR2A may act as a molecular chaperone that keeps APX3 from forming aggregates after translation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- AKR2Ae2
- Outcome
- prevent APX3 from forming aggregates after translatione2
- Qualifier
- molecular chaperonee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana ANKYRIN REPEAT-CONTAINING PROTEIN 2A (AKR2A) interacts with peroxisomal membrane-bound ASCORBATE PEROXIDASE3 (APX3)”
e2“The specificity of the AKR2A-APX3 interaction suggests that AKR2A may function as a molecular chaperone for APX3 because binding of AKR2A to the transmembrane domain can prevent APX3 from forming aggregates after translation”
Claim · model-written summary
AKR2A mutants have reduced steady-state levels of APX3.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- akr2a mutantse3
- Gene
- APX3e3
- Outcome
- steady state levels of APX3e3
- Direction
- reducede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana ANKYRIN REPEAT-CONTAINING PROTEIN 2A (AKR2A) interacts with peroxisomal membrane-bound ASCORBATE PEROXIDASE3 (APX3)”
e3“Analysis of three akr2a mutants indicates that these mutant plants have reduced steady state levels of APX3”
Claim · model-written summary
Reduced AKR2A expression by RNA interference reduces steady-state levels of APX3.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Reduced expression of AKR2A using RNA interferencee4
- Gene
- APX3e4
- Outcome
- steady state levels of APX3e4
- Direction
- reducede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana ANKYRIN REPEAT-CONTAINING PROTEIN 2A (AKR2A) interacts with peroxisomal membrane-bound ASCORBATE PEROXIDASE3 (APX3)”
e4“Reduced expression of AKR2A using RNA interference also leads to reduced steady state levels of APX3 and reduced targeting of APX3 to peroxisomes in plant cells”
Claim · model-written summary
Reduced AKR2A expression by RNA interference reduces targeting of APX3 to peroxisomes in plant cells.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Reduced expression of AKR2A using RNA interferencee4
- Gene
- APX3e4
- Outcome
- targeting of APX3 to peroxisomese4
- Direction
- reducede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana ANKYRIN REPEAT-CONTAINING PROTEIN 2A (AKR2A) interacts with peroxisomal membrane-bound ASCORBATE PEROXIDASE3 (APX3)”
e4“Reduced expression of AKR2A using RNA interference also leads to reduced steady state levels of APX3 and reduced targeting of APX3 to peroxisomes in plant cells”
Claim · model-written summary
AKR2A binds specifically to the chloroplast OEP7.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- OUTER ENVELOPE PROTEIN7 (OEP7)e5
- Outcome
- binds specificallye5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana ANKYRIN REPEAT-CONTAINING PROTEIN 2A (AKR2A) interacts with peroxisomal membrane-bound ASCORBATE PEROXIDASE3 (APX3)”
e5“Since AKR2A also binds specifically to the chloroplast OUTER ENVELOPE PROTEIN7 (OEP7) and is required for the biogenesis of OEP7, AKR2A may serve as a molecular chaperone for OEP7 as well”
Claim · model-written summary
AKR2A is required for the biogenesis of OEP7.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- OEP7e5
- Outcome
- biogenesis of OEP7e5
- Qualifier
- is requirede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana ANKYRIN REPEAT-CONTAINING PROTEIN 2A (AKR2A) interacts with peroxisomal membrane-bound ASCORBATE PEROXIDASE3 (APX3)”
e5“Since AKR2A also binds specifically to the chloroplast OUTER ENVELOPE PROTEIN7 (OEP7) and is required for the biogenesis of OEP7, AKR2A may serve as a molecular chaperone for OEP7 as well”
Claim · model-written summary
AKR2A may act as a molecular chaperone for OEP7 in biogenesis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- AKR2Ae5
- Outcome
- biogenesis of OEP7e5
- Qualifier
- molecular chaperonee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana ANKYRIN REPEAT-CONTAINING PROTEIN 2A (AKR2A) interacts with peroxisomal membrane-bound ASCORBATE PEROXIDASE3 (APX3)”
e5“Since AKR2A also binds specifically to the chloroplast OUTER ENVELOPE PROTEIN7 (OEP7) and is required for the biogenesis of OEP7, AKR2A may serve as a molecular chaperone for OEP7 as well”
Claim · model-written summary
AKR2A plays many important roles in plant cellular metabolism and is essential for plant growth and development.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- AKR2Ae6
- Outcome
- plant growth and developmente6
- Qualifier
- essentiale6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana ANKYRIN REPEAT-CONTAINING PROTEIN 2A (AKR2A) interacts with peroxisomal membrane-bound ASCORBATE PEROXIDASE3 (APX3)”
e6“The pleiotropic phenotype of akr2a mutants indicates that AKR2A plays many important roles in plant cellular metabolism and is essential for plant growth and development”
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