Abstract
Phytate (inositol hexakisphosphate, IP6) is a regulator of intracellular signaling, a highly abundant animal antinutrient, and a phosphate store in plant seeds. Here, we report a requirement for inositol polyphosphate kinases, AtIPK1 and AtIPK2beta, for the later steps of phytate synthesis in Arabidopsis thaliana. Coincident disruption of these kinases nearly ablates seed phytate without accumulation of phytate precursors, increases seed-free phosphate by 10-fold, and has normal seed yield. Additionally, we find a requirement for inositol tetrakisphosphate (IP4)/inositol pentakisphosphate (IP5) 2-kinase activity in phosphate sensing and root hair elongation. Our results define a commercially viable strategy for the genetic engineering of phytate-free grain and provide insights into the role of inositol polyphosphate kinases in phosphate signaling biology.
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Claims 7
Claim · model-written summary
AtIPK1 and AtIPK2beta are required for the later steps of phytate synthesis in Arabidopsis thaliana.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Gene
- AtIPK1 and AtIPK2betae0
- Outcome
- phytate synthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inositol polyphosphate kinases, AtIPK1 and AtIPK2beta, for the later steps of phytate synthesis in Arabidopsis thaliana”
Claim · model-written summary
Coincident disruption of AtIPK1 and AtIPK2beta nearly ablates seed phytate in Arabidopsis thaliana.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Coincident disruption of these kinasese1
- Outcome
- seed phytatee1
- Direction
- nearly ablatese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inositol polyphosphate kinases, AtIPK1 and AtIPK2beta, for the later steps of phytate synthesis in Arabidopsis thaliana”
e1“Coincident disruption of these kinases nearly ablates seed phytate”
Claim · model-written summary
Coincident disruption of these kinases does not lead to accumulation of phytate precursors in Arabidopsis thaliana seeds.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Coincident disruption of these kinasese1
- Outcome
- phytate precursorse1
- Direction
- without accumulatione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inositol polyphosphate kinases, AtIPK1 and AtIPK2beta, for the later steps of phytate synthesis in Arabidopsis thaliana”
e1“Coincident disruption of these kinases nearly ablates seed phytate without accumulation of phytate precursors”
Claim · model-written summary
Coincident disruption of these kinases increases seed-free phosphate by 10-fold in Arabidopsis thaliana.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Coincident disruption of these kinasese1
- Outcome
- seed-free phosphatee1
- Direction
- increasese1
- Value
- 10-folde1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inositol polyphosphate kinases, AtIPK1 and AtIPK2beta, for the later steps of phytate synthesis in Arabidopsis thaliana”
e1“Coincident disruption of these kinases nearly ablates seed phytate without accumulation of phytate precursors, increases seed-free phosphate by 10-fold”
Claim · model-written summary
Coincident disruption of these kinases leaves seed yield normal in Arabidopsis thaliana.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Coincident disruption of these kinasese1
- Outcome
- seed yielde1
- Direction
- normale1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inositol polyphosphate kinases, AtIPK1 and AtIPK2beta, for the later steps of phytate synthesis in Arabidopsis thaliana”
e1“Coincident disruption of these kinases nearly ablates seed phytate without accumulation of phytate precursors, increases seed-free phosphate by 10-fold, and has normal seed yield”
Claim · model-written summary
IP4/IP5 2-kinase activity is required for phosphate sensing and root hair elongation in Arabidopsis thaliana.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- inositol tetrakisphosphate (IP4)/inositol pentakisphosphate (IP5) 2-kinasee1
- Outcome
- phosphate sensing and root hair elongatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inositol polyphosphate kinases, AtIPK1 and AtIPK2beta, for the later steps of phytate synthesis in Arabidopsis thaliana”
e1“Additionally, we find a requirement for inositol tetrakisphosphate (IP4)/inositol pentakisphosphate (IP5) 2-kinase activity in phosphate sensing and root hair elongation”
Claim · model-written summary
The results define a commercially viable strategy for genetic engineering of phytate-free grain.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Outcome
- commercially viable strategy for the genetic engineering of phytate-free graine1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inositol polyphosphate kinases, AtIPK1 and AtIPK2beta, for the later steps of phytate synthesis in Arabidopsis thaliana”
e1“Our results define a commercially viable strategy for the genetic engineering of phytate-free grain”
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