Abstract
We isolated two T-DNA insertion mutants of Arabidopsis thaliana GLUTATHIONE PEROXIDASE3 (ATGPX3) that exhibited a higher rate of water loss under drought stress, higher sensitivity to H(2)O(2) treatment during seed germination and seedling development, and enhanced production of H(2)O(2) in guard cells. By contrast, lines engineered to overexpress ATGPX3 were less sensitive to drought stress than the wild type and displayed less transpirational water loss, which resulted in higher leaf surface temperature. The atgpx3 mutation also disrupted abscisic acid (ABA) activation of calcium channels and the expression of ABA- and stress-responsive genes. ATGPX3 physically interacted with the 2C-type protein phosphatase ABA INSENSITIVE2 (ABI2) and, to a lesser extent, with ABI1. In addition, the redox states of both ATGPX3 and ABI2 were found to be regulated by H(2)O(2). The phosphatase activity of ABI2, measured in vitro, was reduced approximately fivefold by the addition of oxidized ATGPX3. The reduced form of ABI2 was converted to the oxidized form by the addition of oxidized ATGPX3 in vitro, which might mediate ABA and oxidative signaling. These results suggest that ATGPX3 might play dual and distinctive roles in H(2)O(2) homeostasis, acting as a general scavenger and specifically relaying the H(2)O(2) signal as an oxidative signal transducer in ABA and drought stress signaling.
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Claims 14
Claim · model-written summary
Arabidopsis T-DNA insertion mutants of ATGPX3 had a higher rate of water loss under drought stress.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Material
- T-DNA insertion mutantse1
- Treatment
- drought stresse1
- Gene
- ATGPX3e1
- Outcome
- rate of water losse1
- Direction
- highere1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“T-DNA insertion mutants of Arabidopsis thaliana GLUTATHIONE PEROXIDASE3 (ATGPX3) that exhibited a higher rate of water loss under drought stress”
Claim · model-written summary
ATGPX3 mutants were more sensitive to H2O2 treatment during seed germination and seedling development.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- H(2)O(2) treatmente1
- Outcome
- sensitivitye1
- Direction
- highere1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“An Arabidopsis Glutathione Peroxidase”
e1“higher sensitivity to H(2)O(2) treatment during seed germination and seedling development”
Claim · model-written summary
ATGPX3 mutants showed enhanced H2O2 production in guard cells.
- Taxon
- ArabidopsisArabidopsist0
- Material
- T-DNA insertion mutantse2
- Organ / stage
- guard cellse1
- Treatment
- T-DNA insertione2
- Outcome
- production of H(2)O(2)e1
- Direction
- enhancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“An Arabidopsis Glutathione Peroxidase”
e1“enhanced production of H(2)O(2) in guard cells”
e2“We isolated two T-DNA insertion mutants of Arabidopsis thaliana GLUTATHIONE PEROXIDASE3 (ATGPX3)”
Claim · model-written summary
Lines overexpressing ATGPX3 were less sensitive to drought stress than the wild type.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- overexpress ATGPX3e1
- Gene
- ATGPX3e1
- Outcome
- sensitive to drought stresse1
- Direction
- lesse1
- Comparator
- wild typee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“An Arabidopsis Glutathione Peroxidase”
e1“lines engineered to overexpress ATGPX3 were less sensitive to drought stress than the wild type”
Claim · model-written summary
Lines overexpressing ATGPX3 displayed less transpirational water loss than the wild type.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- overexpress ATGPX3e2
- Outcome
- transpirational water losse1
- Direction
- lesse1
- Comparator
- wild typee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“An Arabidopsis Glutathione Peroxidase”
e1“displayed less transpirational water loss”
e2“lines engineered to overexpress ATGPX3 were less sensitive to drought stress than the wild type”
Claim · model-written summary
Lines overexpressing ATGPX3 had higher leaf surface temperature than the wild type.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- overexpress ATGPX3e2
- Outcome
- leaf surface temperaturee1
- Direction
- highere1
- Comparator
- wild typee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“An Arabidopsis Glutathione Peroxidase”
e1“which resulted in higher leaf surface temperature”
e2“lines engineered to overexpress ATGPX3 were less sensitive to drought stress than the wild type”
Claim · model-written summary
The ATGPX3 mutation disrupted ABA activation of calcium channels in Arabidopsis.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- atgpx3 mutatione1
- Gene
- atgpx3e1
- Outcome
- activation of calcium channelse1
- Direction
- disruptede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“An Arabidopsis Glutathione Peroxidase”
e1“The atgpx3 mutation also disrupted abscisic acid (ABA) activation of calcium channels and the expression of ABA- and stress-responsive genes”
Claim · model-written summary
The ATGPX3 mutation disrupted expression of ABA- and stress-responsive genes in Arabidopsis.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- atgpx3 mutatione1
- Outcome
- expression of ABA- and stress-responsive genese1
- Direction
- disruptede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“An Arabidopsis Glutathione Peroxidase”
e1“The atgpx3 mutation also disrupted abscisic acid (ABA) activation of calcium channels and the expression of ABA- and stress-responsive genes”
Claim · model-written summary
ATGPX3 physically interacted with ABI2 in Arabidopsis.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- ABI2e1
- Outcome
- physically interacted withe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“An Arabidopsis Glutathione Peroxidase”
e1“ATGPX3 physically interacted with the 2C-type protein phosphatase ABA INSENSITIVE2 (ABI2) and, to a lesser extent, with ABI1”
Claim · model-written summary
ATGPX3 physically interacted with ABI1, to a lesser extent than with ABI2.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- ABI1e1
- Outcome
- physically interacted withe1
- Qualifier
- to a lesser extente1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“An Arabidopsis Glutathione Peroxidase”
e1“ATGPX3 physically interacted with the 2C-type protein phosphatase ABA INSENSITIVE2 (ABI2) and, to a lesser extent, with ABI1”
Claim · model-written summary
Redox states of ATGPX3 and ABI2 were regulated by H2O2.
- Taxon
- ArabidopsisArabidopsist0
- Compound
- H(2)O(2)e1
- Outcome
- redox statese1
- Direction
- regulatede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“An Arabidopsis Glutathione Peroxidase”
e1“the redox states of both ATGPX3 and ABI2 were found to be regulated by H(2)O(2)”
Claim · model-written summary
Oxidized ATGPX3 reduced ABI2 phosphatase activity approximately fivefold in vitro.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- addition of oxidized ATGPX3e1
- Gene
- ABI2e1
- Outcome
- phosphatase activity of ABI2e1
- Direction
- reducede1
- Value
- approximately fivefolde1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“An Arabidopsis Glutathione Peroxidase”
e1“The phosphatase activity of ABI2, measured in vitro, was reduced approximately fivefold by the addition of oxidized ATGPX3”
Claim · model-written summary
Oxidized ATGPX3 converted reduced ABI2 to its oxidized form in vitro.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- addition of oxidized ATGPX3e1
- Gene
- ABI2e1
- Outcome
- reduced form of ABI2e1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“An Arabidopsis Glutathione Peroxidase”
e1“The reduced form of ABI2 was converted to the oxidized form by the addition of oxidized ATGPX3 in vitro”
Claim · model-written summary
ATGPX3 might act in ABA and drought stress signaling by relaying H2O2 as an oxidative signal while also scavenging H2O2.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- ATGPX3e1
- Outcome
- ABA and drought stress signalinge1
- Qualifier
- relaying the H(2)O(2) signal as an oxidative signal transducere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“An Arabidopsis Glutathione Peroxidase”
e1“ATGPX3 might play dual and distinctive roles in H(2)O(2) homeostasis, acting as a general scavenger and specifically relaying the H(2)O(2) signal as an oxidative signal transducer in ABA and drought stress signaling”
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