Abstract
Manganese (Mn) is an essential micronutrient for plants, but is toxic when present in excess. The rice plant (Oryza sativa L.) accumulates high concentrations of Mn in the aerial parts; however, the molecular basis for Mn tolerance is poorly understood. In the present study, genes encoding Mn tolerance were screened for by expressing cDNAs of genes from rice shoots in Saccharomyces cerevisiae. A gene encoding a cation diffusion facilitator (CDF) family member, OsMTP8.1, was isolated, and its expression was found to enhance Mn accumulation and tolerance in S. cerevisiae. In plants, OsMTP8.1 and its transcript were mainly detected in shoots. High or low supply of Mn moderately induced an increase or decrease in the accumulation of OsMTP8.1, respectively. OsMTP8.1 was detected in all cells of leaf blades through immunohistochemistry. OsMTP8.1 fused to green fluorescent protein was localized to the tonoplast. Disruption of OsMTP8.1 resulted in decreased chlorophyll levels, growth inhibition in the presence of high concentrations of Mn, and decreased accumulation of Mn in shoots and roots. However, there was no difference in the accumulation of other metals, including Zn, Cu, Fe, Mg, Ca, and K. These results suggest that OsMTP8.1 is an Mn-specific transporter that sequesters Mn into vacuoles in rice and is required for Mn tolerance in shoots.
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Claims 11
Claim · model-written summary
Expression of OsMTP8.1 in yeast enhanced Mn accumulation and tolerance.
- Taxon
- rice
Oryza sativat0 - Treatment
- its expressione1
- Gene
- OsMTP8.1e1
- Outcome
- Mn accumulation and tolerancee1
- Direction
- enhancee1
- Qualifier
- in S. cerevisiaee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family”
e1“A gene encoding a cation diffusion facilitator (CDF) family member, OsMTP8.1, was isolated, and its expression was found to enhance Mn accumulation and tolerance in S. cerevisiae”
Claim · model-written summary
OsMTP8.1 and its transcript are mainly detected in rice shoots.
- Taxon
- riceOryza sativat0
- Organ / stage
- shootse2
- Gene
- OsMTP8.1e2
- Outcome
- OsMTP8.1 and its transcripte2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family”
e2“In plants, OsMTP8.1 and its transcript were mainly detected in shoots”
Claim · model-written summary
High Mn supply moderately increased the accumulation of OsMTP8.1 in rice.
- Taxon
- riceOryza sativat0
- Treatment
- High or low supply of Mne3
- Gene
- OsMTP8.1e3
- Outcome
- accumulation of OsMTP8.1e3
- Direction
- increasee3
- Qualifier
- moderatelye3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family”
e3“High or low supply of Mn moderately induced an increase or decrease in the accumulation of OsMTP8.1, respectively”
Claim · model-written summary
Low Mn supply moderately decreased the accumulation of OsMTP8.1 in rice.
- Taxon
- riceOryza sativat0
- Treatment
- High or low supply of Mne3
- Gene
- OsMTP8.1e3
- Outcome
- accumulation of OsMTP8.1e3
- Direction
- decreasee3
- Qualifier
- moderatelye3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family”
e3“High or low supply of Mn moderately induced an increase or decrease in the accumulation of OsMTP8.1, respectively”
Claim · model-written summary
OsMTP8.1 is detected in all cells of rice leaf blades.
- Taxon
- riceOryza sativat0
- Organ / stage
- leaf bladese4
- Gene
- OsMTP8.1e4
- Outcome
- detected in all cells of leaf bladese4
- Qualifier
- immunohistochemistrye4
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family”
e4“OsMTP8.1 was detected in all cells of leaf blades through immunohistochemistry”
Claim · model-written summary
OsMTP8.1 fused to GFP localizes to the tonoplast.
- Taxon
- riceOryza sativat0
- Gene
- OsMTP8.1e5
- Outcome
- localized to the tonoplaste5
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family”
e5“OsMTP8.1 fused to green fluorescent protein was localized to the tonoplast”
Claim · model-written summary
Disruption of OsMTP8.1 decreased chlorophyll levels in rice.
- Taxon
- riceOryza sativat0
- Treatment
- Disruption of OsMTP8.1e6
- Gene
- OsMTP8.1e6
- Outcome
- chlorophyll levelse6
- Direction
- decreasede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family”
e6“Disruption of OsMTP8.1 resulted in decreased chlorophyll levels, growth inhibition in the presence of high concentrations of Mn, and decreased accumulation of Mn in shoots and roots”
Claim · model-written summary
Disruption of OsMTP8.1 inhibited rice growth in the presence of high Mn.
- Taxon
- riceOryza sativat0
- Treatment
- Disruption of OsMTP8.1e6
- Outcome
- growthe6
- Direction
- inhibitione6
- Qualifier
- in the presence of high concentrations of Mne6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family”
e6“Disruption of OsMTP8.1 resulted in decreased chlorophyll levels, growth inhibition in the presence of high concentrations of Mn, and decreased accumulation of Mn in shoots and roots”
Claim · model-written summary
Disruption of OsMTP8.1 decreased Mn accumulation in rice shoots and roots.
- Taxon
- riceOryza sativat0
- Treatment
- Disruption of OsMTP8.1e6
- Outcome
- accumulation of Mn in shoots and rootse6
- Direction
- decreasede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family”
e6“Disruption of OsMTP8.1 resulted in decreased chlorophyll levels, growth inhibition in the presence of high concentrations of Mn, and decreased accumulation of Mn in shoots and roots”
Claim · model-written summary
Disruption of OsMTP8.1 did not change the accumulation of other metals (Zn, Cu, Fe, Mg, Ca, K) in rice.
- Taxon
- riceOryza sativat0
- Treatment
- Disruption of OsMTP8.1e6
- Outcome
- accumulation of other metalse7
- Direction
- no differencee7
- Qualifier
- including Zn, Cu, Fe, Mg, Ca, and Ke7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family”
e6“Disruption of OsMTP8.1 resulted in decreased chlorophyll levels, growth inhibition in the presence of high concentrations of Mn, and decreased accumulation of Mn in shoots and roots”
e7“However, there was no difference in the accumulation of other metals, including Zn, Cu, Fe, Mg, Ca, and K”
Claim · model-written summary
OsMTP8.1 is an Mn-specific transporter that sequesters Mn into vacuoles and is required for Mn tolerance in rice shoots (author interpretation).
- Taxon
- riceOryza sativat0
- Gene
- OsMTP8.1e1
- Outcome
- Mn tolerance in shootse1
- Qualifier
- sequesters Mn into vacuolese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family”
e1“These results suggest that OsMTP8.1 is an Mn-specific transporter that sequesters Mn into vacuoles in rice and is required for Mn tolerance in shoots”
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