Abstract
We have cloned an LTP gene (PoLTP1) from poplar (Populus alba × P. tremula var. glandulosa) suspension cells and examined changes in its expression levels in response to various stresses and ABA treatment. The full-length PoLTP1 cDNA clone encodes a polypeptide of 116 amino acids with typical characteristics of LTPs, notably a conserved arrangement of cysteine residues. Southern blot analysis indicate that two or three copies of the PoLTP1 are present in the genome of the investigated hybrid poplar. In addition, northern analysis of samples from soil-grown plants indicate that PoLTP1 is tissue-specifically expressed in the leaves and flowers. The gene is significantly up-regulated by treatment with mannitol, NaCl and ABA, but not by either cold or wounding. These results indicate that PoLTP1 is involved in osmotic stress responses in poplar plants and suspension cells.
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Claims 10
Claim · model-written summary
PoLTP1 encodes a 116-amino-acid polypeptide.
Not reportedTaxon
Verbatim
e0“The full-length PoLTP1 cDNA clone encodes a polypeptide of 116 amino acids with typical characteristics of LTPs, notably a conserved arrangement of cysteine residues.”
Claim · model-written summary
PoLTP1 protein has a conserved arrangement of cysteine residues.
Not reportedTaxon
Verbatim
e0“The full-length PoLTP1 cDNA clone encodes a polypeptide of 116 amino acids with typical characteristics of LTPs, notably a conserved arrangement of cysteine residues.”
Claim · model-written summary
The hybrid poplar genome has two or three copies of PoLTP1.
PopulusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two or three copies of the PoLTP1 are present in the genome of the investigated hybrid poplar.”
Claim · model-written summary
PoLTP1 is expressed in leaves and flowers.
Not reportedTaxon
Verbatim
e0“PoLTP1 is tissue-specifically expressed in the leaves and flowers.”
Claim · model-written summary
Mannitol significantly upregulated PoLTP1.
Not reportedTaxonComparator
Verbatim
e0“The gene is significantly up-regulated by treatment with mannitol, NaCl and ABA, but not by either cold or wounding.”
Claim · model-written summary
NaCl significantly upregulated PoLTP1.
Not reportedTaxonComparator
Verbatim
e0“The gene is significantly up-regulated by treatment with mannitol, NaCl and ABA, but not by either cold or wounding.”
Claim · model-written summary
ABA significantly upregulated PoLTP1.
Not reportedTaxonComparator
Verbatim
e0“The gene is significantly up-regulated by treatment with mannitol, NaCl and ABA, but not by either cold or wounding.”
Claim · model-written summary
Cold did not upregulate PoLTP1.
Not reportedTaxonComparatorDirection
Verbatim
e0“The gene is significantly up-regulated by treatment with mannitol, NaCl and ABA, but not by either cold or wounding.”
Claim · model-written summary
Wounding did not upregulate PoLTP1.
Not reportedTaxonComparatorDirection
Verbatim
e0“The gene is significantly up-regulated by treatment with mannitol, NaCl and ABA, but not by either cold or wounding.”
Claim · model-written summary
The authors infer PoLTP1 involvement in poplar osmotic stress responses.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results indicate that PoLTP1 is involved in osmotic stress responses in poplar plants and suspension cells.”
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.