Abstract
BACKGROUND: R2R3-MYB transcription factors (TFs) play important roles in plant growth and development, and response to biotic and abiotic stresses. However, their regulatory mechanisms in wound-induced anthocyanin biosynthesis in woody plants are largely unknown. RESULTS: In this work, we report that expression of anthocyanin biosynthesis genes (ABGs) were activated by PdMYB118, a MYB TF encoding gene from Populus deltoids, and the activation of PdMYB118 was significantly enhanced by PdTT8, a bHLH protein, through its direct interaction with PdMYB118. PdMYB118 and some ABGs were evidently induced by wound induction and methyl jasmonate (MeJA) treatment. Overexpression of PdMYB118 promoted anthocyanin accumulation in transgenic poplar upon wound induction. Furthermore, a poplar JASMONATE ZIM-domain (JAZ) protein, PtrJAZ1, repressed the transcriptional function of PdMYB118/PdTT8 complex by binding to PdTT8, and wound stimulated the biosynthesis of jasmonic acid (JA) and the degradation of PtrJAZ1. CONCLUSIONS: Based on these observations, we proposed that PtrJAZ1 degradation triggered the expression of ABGs, leading to increased biosynthesis of anthocyanins in the wounded leaves of transgenic poplar. Therefore, our findings not only illustrate the crucial role of PdMYB118 in wound-induced anthocyanin biosynthesis in poplar, but also provide a molecular basis for the genetic engineering of colorful tree species.
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Claims 7
Claim · model-written summary
PdMYB118 activated expression of anthocyanin biosynthesis genes in poplar.
- Taxon
- Populus deltoidse1
- Treatment
- PdMYB118e1
- Gene
- PdMYB118e1
- Outcome
- expression of anthocyanin biosynthesis genese1
- Direction
- activatede1
Not reportedComparator
Verbatim
e1“expression of anthocyanin biosynthesis genes (ABGs) were activated by PdMYB118, a MYB TF encoding gene from Populus deltoids, and the activation of PdMYB118 was significantly enhanced by PdTT8, a bHLH protein, through its direct interaction with PdMYB118”
Claim · model-written summary
PdTT8 significantly enhanced the activation of PdMYB118 through direct interaction.
- Taxon
- Populus deltoidse1
- Treatment
- PdTT8e1
- Gene
- PdTT8e1
- Outcome
- the activation of PdMYB118e1
- Direction
- significantly enhancede1
Not reportedComparator
Verbatim
e1“expression of anthocyanin biosynthesis genes (ABGs) were activated by PdMYB118, a MYB TF encoding gene from Populus deltoids, and the activation of PdMYB118 was significantly enhanced by PdTT8, a bHLH protein, through its direct interaction with PdMYB118”
Claim · model-written summary
PdMYB118 and some anthocyanin biosynthesis genes were evidently induced by wound induction and methyl jasmonate treatment.
- Treatment
- wound induction and methyl jasmonate (MeJA) treatmente2
- Gene
- PdMYB118e2
- Outcome
- PdMYB118 and some ABGse2
- Direction
- inducede2
PopulusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“PdMYB118 and some ABGs were evidently induced by wound induction and methyl jasmonate (MeJA) treatment”
e3“Overexpression of PdMYB118 promoted anthocyanin accumulation in transgenic poplar upon wound induction”
Claim · model-written summary
Overexpression of PdMYB118 promoted anthocyanin accumulation in transgenic poplar upon wound induction.
- Material
- transgenic poplare3
- Treatment
- Overexpression of PdMYB118e3
- Gene
- PdMYB118e3
- Outcome
- anthocyanin accumulatione3
- Direction
- promotede3
- Qualifier
- upon wound inductione3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Overexpression of PdMYB118 promoted anthocyanin accumulation in transgenic poplar upon wound induction”
Claim · model-written summary
PtrJAZ1 represses the transcriptional function of the PdMYB118/PdTT8 complex by binding PdTT8.
- Gene
- PtrJAZ1e4
- Outcome
- the transcriptional function of PdMYB118/PdTT8 complexe4
- Direction
- repressede4
- Qualifier
- by binding to PdTT8e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“a poplar JASMONATE ZIM-domain (JAZ) protein, PtrJAZ1, repressed the transcriptional function of PdMYB118/PdTT8 complex by binding to PdTT8, and wound stimulated the biosynthesis of jasmonic acid (JA) and the degradation of PtrJAZ1”
Claim · model-written summary
Wounding stimulated jasmonic acid biosynthesis and the degradation of PtrJAZ1 in poplar.
- Treatment
- wounde4
- Gene
- PtrJAZ1e4
- Outcome
- biosynthesis of jasmonic acid (JA) and the degradation of PtrJAZ1e4
- Direction
- stimulatede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“a poplar JASMONATE ZIM-domain (JAZ) protein, PtrJAZ1, repressed the transcriptional function of PdMYB118/PdTT8 complex by binding to PdTT8, and wound stimulated the biosynthesis of jasmonic acid (JA) and the degradation of PtrJAZ1”
Claim · model-written summary
PtrJAZ1 degradation is proposed to trigger ABG expression and increased anthocyanin biosynthesis in wounded transgenic poplar leaves.
- Gene
- PtrJAZ1e5
- Outcome
- increased biosynthesis of anthocyaninse5
- Direction
- increasede5
- Qualifier
- PtrJAZ1 degradation triggered the expression of ABGse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“PtrJAZ1 degradation triggered the expression of ABGs, leading to increased biosynthesis of anthocyanins in the wounded leaves of transgenic poplar”
The same questions in other papers
(-)-methyl jasmonate · molecular: expression · Populus (poplar)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
1 paper more in this group
See all 5 papers →mechanical · molecular: expression · Populus (poplar)Positive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.