Abstract
The ZFHD recognition sequence (ZFHDRS) and NAC recognition sequence (NACRS) play an important role in the dehydration-inducible expression of the Arabidopsisthaliana EARLY RESPONSIVETO DEHYDRATION STRESS 1 (ERD1) gene. Using the yeast one-hybrid system, we isolated a cDNA encoding the ZFHD1 transcriptional activator that specifically binds to the 62 bp promoter region of ERD1, which contains the ZFHDRS. Both in vitro and in vivo analyses confirmed specific binding of the ZFHD1 to ZFHDRS, and the expression of ZFHD1 was induced by drought, high salinity and abscisic acid. The DNA-binding and activation domains of ZFHD1 were localized on the C-terminal homeodomain and N-terminal zinc finger domain, respectively. Microarray analysis of transgenic plants over-expressing ZFHD1 revealed that several stress-inducible genes were upregulated in the transgenic plants. Transgenic plants exhibited a smaller morphological phenotype and had a significant improvement of drought stress tolerance. Using the yeast two-hybrid system, we detected an interaction between ZFHD1 and NACRS-binding NAC proteins. Moreover, co-over-expression of the ZFHD1 and NAC genes restored the morphological phenotype of the transgenic plants to a near wild-type state and enhanced expression of ERD1 in both Arabidopsis T87 protoplasts and transgenic Arabidopsis plants.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
ZFHD1 bound the ERD1 promoter ZFHDRS specifically.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- ZFHD1e0
- Outcome
- specifically binds to the 62 bp promoter region of ERD1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ZFHD1 transcriptional activator that specifically binds to the 62 bp promoter region of ERD1, which contains the ZFHDRS”
t0“Co‐expression of the stress‐inducible zinc finger homeodomain ZFHD1 and NAC transcription factors enhances expression of the ERD1 gene in Arabidopsis”
Claim · model-written summary
Drought, salinity and abscisic acid induced ZFHD1 expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- drought, high salinity and abscisic acide0
- Gene
- ZFHD1e0
- Outcome
- expression of ZFHD1e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of ZFHD1 was induced by drought, high salinity and abscisic acid”
t0“Co‐expression of the stress‐inducible zinc finger homeodomain ZFHD1 and NAC transcription factors enhances expression of the ERD1 gene in Arabidopsis”
Claim · model-written summary
ZFHD1 DNA binding and activation mapped to its C-terminal homeodomain and N-terminal zinc finger domain.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- ZFHD1e0
- Outcome
- DNA-binding and activation domainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The DNA-binding and activation domains of ZFHD1 were localized on the C-terminal homeodomain and N-terminal zinc finger domain, respectively”
t0“Co‐expression of the stress‐inducible zinc finger homeodomain ZFHD1 and NAC transcription factors enhances expression of the ERD1 gene in Arabidopsis”
Claim · model-written summary
Several stress-inducible genes were upregulated in ZFHD1 overexpressors.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- transgenic plantse0
- Gene
- ZFHD1t0
- Outcome
- stress-inducible genese0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“several stress-inducible genes were upregulated in the transgenic plants”
t0“Co‐expression of the stress‐inducible zinc finger homeodomain ZFHD1 and NAC transcription factors enhances expression of the ERD1 gene in Arabidopsis”
Claim · model-written summary
ZFHD1 overexpressors had a smaller morphology.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Transgenic plantse0
- Outcome
- morphological phenotypee0
- Direction
- smallere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic plants exhibited a smaller morphological phenotype”
t0“Co‐expression of the stress‐inducible zinc finger homeodomain ZFHD1 and NAC transcription factors enhances expression of the ERD1 gene in Arabidopsis”
Claim · model-written summary
ZFHD1 overexpressors significantly improved drought tolerance.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Transgenic plantse0
- Outcome
- drought stress tolerancee0
- Direction
- improvemente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic plants exhibited a smaller morphological phenotype and had a significant improvement of drought stress tolerance”
t0“Co‐expression of the stress‐inducible zinc finger homeodomain ZFHD1 and NAC transcription factors enhances expression of the ERD1 gene in Arabidopsis”
Claim · model-written summary
ZFHD1 interacted with NACRS-binding NAC proteins.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- ZFHD1e0
- Outcome
- interaction between ZFHD1 and NACRS-binding NAC proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we detected an interaction between ZFHD1 and NACRS-binding NAC proteins”
t0“Co‐expression of the stress‐inducible zinc finger homeodomain ZFHD1 and NAC transcription factors enhances expression of the ERD1 gene in Arabidopsis”
Claim · model-written summary
Co-overexpression of ZFHD1 and NAC restored morphology near wild type.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- co-over-expression of the ZFHD1 and NAC genese0
- Gene
- ZFHD1 and NAC genese0
- Outcome
- morphological phenotypee0
- Direction
- restorede0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“co-over-expression of the ZFHD1 and NAC genes restored the morphological phenotype of the transgenic plants to a near wild-type state”
t0“Co‐expression of the stress‐inducible zinc finger homeodomain ZFHD1 and NAC transcription factors enhances expression of the ERD1 gene in Arabidopsis”
Claim · model-written summary
Co-overexpression of ZFHD1 and NAC enhanced ERD1 expression.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- co-over-expression of the ZFHD1 and NAC genese0
- Gene
- ERD1e0
- Outcome
- expression of ERD1e0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“co-over-expression of the ZFHD1 and NAC genes restored the morphological phenotype of the transgenic plants to a near wild-type state and enhanced expression of ERD1 in both Arabidopsis T87 protoplasts and transgenic Arabidopsis plants”
The same questions in other papers
salinity · molecular: expression · ArabidopsisPositive and negative results
19 papers in the same direction as this paper · 18 in the opposite direction
- negative
- no effect
- negative
34 papers more in this group
See all 38 papers →drought · molecular: expression · ArabidopsisPositive and negative results
38 papers in the same direction as this paper · 8 in the opposite direction
- positive
- positive
- negative
43 papers more in this group
See all 47 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.