Abstract
BACKGROUND: γ-Aminobutyric acid (GABA) can be synthesised by the GABA shunt and polyamine degradation pathway in plants under hypoxia stress and lower temperature. The hypoxia germination freeze-thawing incubation was used as a new technique for accumulating more GABA in soybean. RESULTS: Results showed that glutamate decarboxylase (GAD) and diamine oxidase (DAO) activity as well as GABA content increased during germination within 24 h under hypoxia. However, the contents of dry matters and protein decreased. When the hypoxia-treated sprouts were frozen at -18 °C for 12 h and thawed at 25 °C for 6 h, GABA content increased drastically to 7.21-fold of the non-frozen sprouts. Subsequently, the freeze-thawing sprouts were ground into homogenates and incubated. GABA content was 14.20-fold of the only-soaked seeds when homogenates was incubated at 45 °C for 80 min within 400 µmol L(-1) pyridoxine (VB6) (pH 6.5). CONCLUSION: The hypoxia germination freeze-thawing incubation was an effective method for accumulating GABA in soybean. During incubation, DAO was more important for GABA formation in homogenate of germinated soybean compared with GAD.
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Claims 6
Claim · model-written summary
GAD and DAO activity and GABA content increased during germination within 24 h under hypoxia in soybean.
- Taxon
- soybean
Glycine maxt0 - Organ / stage
- germinatione0
- Treatment
- hypoxiae0
- Gene
- glutamate decarboxylase (GAD)e0
- Outcome
- glutamate decarboxylase (GAD) and diamine oxidase (DAO) activity as well as GABA contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in soybean by hypoxia germination”
e0“Results showed that glutamate decarboxylase (GAD) and diamine oxidase (DAO) activity as well as GABA content increased during germination within 24 h under hypoxia”
Claim · model-written summary
Contents of dry matter and protein decreased during hypoxia germination of soybean.
- Taxon
- soybeanGlycine maxt0
- Treatment
- hypoxiae0
- Outcome
- contents of dry matters and proteine1
- Direction
- decreasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in soybean by hypoxia germination”
e0“Results showed that glutamate decarboxylase (GAD) and diamine oxidase (DAO) activity as well as GABA content increased during germination within 24 h under hypoxia”
e1“However, the contents of dry matters and protein decreased”
Claim · model-written summary
GABA content increased drastically to 7.21-fold after freezing and thawing of hypoxia-treated soybean sprouts.
- Taxon
- soybeanGlycine maxt0
- Treatment
- frozen at -18 °C for 12 h and thawed at 25 °C for 6 he2
- Outcome
- GABA contente2
- Direction
- increased drasticallye2
- Value
- 7.21-folde2
- Comparator
- non-frozen sproutse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in soybean by hypoxia germination”
e2“When the hypoxia-treated sprouts were frozen at -18 °C for 12 h and thawed at 25 °C for 6 h, GABA content increased drastically to 7.21-fold of the non-frozen sprouts”
Claim · model-written summary
GABA content in homogenates was 14.20-fold of only-soaked seeds after incubation at 45 °C for 80 min.
- Taxon
- soybeanGlycine maxt0
- Treatment
- incubated at 45 °C for 80 mine3
- Outcome
- GABA contente3
- Direction
- increaseinferrede3
- Value
- 14.20-folde3
- Comparator
- only-soaked seedse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in soybean by hypoxia germination”
e3“GABA content was 14.20-fold of the only-soaked seeds when homogenates was incubated at 45 °C for 80 min within 400 µmol L(-1) pyridoxine (VB6) (pH 6.5)”
Claim · model-written summary
Diamine oxidase was more important than glutamate decarboxylase for GABA formation in homogenates of germinated soybean.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in soybean by hypoxia germination”
e4“During incubation, DAO was more important for GABA formation in homogenate of germinated soybean compared with GAD”
Claim · model-written summary
The hypoxia germination freeze-thawing incubation was an effective method for accumulating GABA in soybean.
- Taxon
- soybeanGlycine maxt0
- Outcome
- accumulating GABA in soybeane5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in soybean by hypoxia germination”
e5“The hypoxia germination freeze-thawing incubation was an effective method for accumulating GABA in soybean”
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