Abstract
Flooding stress has a negative impact on soybean cultivation because it severely impairs growth and development. To understand the flooding responsive mechanism in early stage soybeans, a glycoproteomic technique was used. Two-day-old soybeans were treated with flooding for 2 days and roots were collected. Globally, the accumulation level of glycoproteins, as revealed by cross-reaction with concanavalin A decreased by 2 days of flooding stress. Glycoproteins were enriched from total protein extracts using concanavalin A lectin resin and analyzed using a gel-free proteomic technique. One-hundred eleven and 69 glycoproteins were identified without and with 2 days of flooding stress, respectively. Functional categorization of these identified glycoproteins indicated that the accumulation level of proteins related to protein degradation, cell wall, and glycolysis increased, while stress-related proteins decreased under flooding stress. Also the accumulation level of glycoproteins localized in the secretory pathway decreased under flooding stress. Out of 23 common glycoproteins between control and flooding conditions, peroxidases and glycosyl hydrolases were decreased by 2 days of flooding stress. mRNA expression levels of proteins in the endoplasmic reticulum and N-glycosylation related proteins were downregulated by flooding stress. These results suggest that flooding might negatively affect the process of N-glycosylation of proteins related to stress and protein degradation; however glycoproteins involved in glycolysis are activated.
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Claims 9
Claim · model-written summary
Flooding reduced overall glycoprotein accumulation in soybean roots.
- Taxon
- soybean
Glycine maxt0 - Treatment
- 2 days of flooding stresse0
- Outcome
- accumulation level of glycoproteinse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the accumulation level of glycoproteins, as revealed by cross-reaction with concanavalin A decreased by 2 days of flooding stress”
t0“soybean root”
Claim · model-written summary
The number of identified glycoproteins fell from 111 to 69 under flooding.
- Taxon
- soybeanGlycine maxt0
- Outcome
- glycoproteins were identifiede0
- Value
- 69 glycoproteinse0
- Qualifier
- One-hundred elevene0
- Comparator
- withoute0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One-hundred eleven and 69 glycoproteins were identified without and with 2 days of flooding stress, respectively.”
t0“soybean root”
Claim · model-written summary
Protein degradation, cell wall and glycolysis glycoproteins increased under flooding.
- Taxon
- soybeanGlycine maxt0
- Treatment
- flooding stresse0
- Outcome
- accumulation level of proteins related to protein degradation, cell wall, and glycolysise0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the accumulation level of proteins related to protein degradation, cell wall, and glycolysis increased, while stress-related proteins decreased under flooding stress”
t0“soybean root”
Claim · model-written summary
Stress-related protein accumulation decreased under flooding.
- Taxon
- soybeanGlycine maxt0
- Treatment
- flooding stresse0
- Outcome
- stress-related proteinse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stress-related proteins decreased under flooding stress”
t0“soybean root”
Claim · model-written summary
Secretory pathway glycoproteins decreased under flooding.
- Taxon
- soybeanGlycine maxt0
- Treatment
- flooding stresse0
- Outcome
- accumulation level of glycoproteins localized in the secretory pathwaye0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“accumulation level of glycoproteins localized in the secretory pathway decreased under flooding stress”
t0“soybean root”
Claim · model-written summary
Peroxidases and glycosyl hydrolases decreased after flooding.
- Taxon
- soybeanGlycine maxt0
- Treatment
- 2 days of flooding stresse0
- Outcome
- peroxidases and glycosyl hydrolasese0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“peroxidases and glycosyl hydrolases were decreased by 2 days of flooding stress”
t0“soybean root”
Claim · model-written summary
Endoplasmic reticulum and N-glycosylation related mRNAs were downregulated under flooding.
- Taxon
- soybeanGlycine maxt0
- Treatment
- flooding stresse0
- Outcome
- mRNA expression levels of proteins in the endoplasmic reticulum and N-glycosylation related proteinse0
- Direction
- downregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mRNA expression levels of proteins in the endoplasmic reticulum and N-glycosylation related proteins were downregulated by flooding stress”
t0“soybean root”
Claim · model-written summary
Authors suggest flooding may impair N-glycosylation of stress and degradation proteins.
- Taxon
- soybeanGlycine maxt0
- Treatment
- floodinge0
- Outcome
- process of N-glycosylation of proteins related to stress and protein degradatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“flooding might negatively affect the process of N-glycosylation of proteins related to stress and protein degradation”
t0“soybean root”
Claim · model-written summary
Authors suggest glycoproteins involved in glycolysis are activated under flooding.
- Taxon
- soybeanGlycine maxt0
- Outcome
- glycoproteins involved in glycolysis are activatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“glycoproteins involved in glycolysis are activated”
t0“soybean root”
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