Abstract
Abstract The plasmid pME6863, carrying the aiiA gene from the soil bacterium Bacillus sp. A24 that encodes a lactonase enzyme able to degrade N-acyl-homoserine lactones (AHLs), was introduced into the rhizosphere isolate Pseudomonas fluorescens P3. This strain is not an effective biological control agent against plant pathogens. The transformant P. fluorescens P3/pME6863 acquired the ability to degrade AHLs. In planta, P. fluorescens P3/pME6863 significantly reduced potato soft rot caused by Erwinia carotovora and crown gall of tomato caused by Agrobacterium tumefaciens to a similar level as Bacillus sp. A24. Little or no disease reduction was observed for the wild-type strain P3 carrying the vector plasmid without aiiA. Suppression of potato soft rot was observed even when the AHL-degrading P. fluorescens P3/pME6863 was applied to tubers 2 days after the pathogen, indicating that biocontrol was not only preventive but also curative. When antagonists were applied individually with the bacterial plant pathogens, biocontrol activity of the AHL degraders was greater than that observed with several Pseudomonas 2,4-diacetylphloroglucinol-producing strains and with Pseudomonas chlororaphis PCL1391, which relies on production of phenazine antibiotic for disease suppression. Phenazine production by this well characterized biological control strain P. chlororaphis PCL1391 is regulated by AHL-mediated quorum sensing. When P. chlororaphis PCL1391 was co-inoculated with P. fluorescens P3/pME6863 in a strain mixture, the AHL degrader interfered with the normally excellent ability of the antibiotic producer to suppress tomato vascular wilt caused by Fusarium oxysporum f. sp. lycopersici. Our results demonstrate AHL degradation as a novel biocontrol mechanism, but also demonstrate the potential for non-target interactions that can interfere with the biocontrol efficacy of other strains.
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Claims 7
Claim · model-written summary
The transformed P. fluorescens strain acquired AHL degradation ability.
- Taxon
- potato
Solanum tuberosumt0 - Interactor
- P. fluorescens P3/pME6863
.JPG?width=60)
Pseudomonas fluorescense0biocontrol_agent - Outcome
- P. fluorescens P3/pME6863 acquired the ability to degrade AHLse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.JPG?width=500)
Verbatim
e0“The transformant P. fluorescens P3/pME6863 acquired the ability to degrade AHLs”
t0“potato soft rot caused by Erwinia carotovora”
Claim · model-written summary
The transformed strain significantly reduced Erwinia soft rot in potato.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- P. fluorescens P3/pME6863e0
- Interactor
- Erwinia carotovora
Pectobacterium carotovorume0pathogen - Second interactor
- P. fluorescens P3/pME6863Pseudomonas fluorescense0biocontrol_agent
- Outcome
- potato soft rot caused by Erwinia carotovorae0
- Direction
- reducede0
- Comparator
- Bacillus sp. A24e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P. fluorescens P3/pME6863 significantly reduced potato soft rot caused by Erwinia carotovora and crown gall of tomato caused by Agrobacterium tumefaciens to a similar level as Bacillus sp. A24”
Claim · model-written summary
The transformed strain significantly reduced Agrobacterium crown gall in tomato.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- P. fluorescens P3/pME6863e0
- Interactor
- Agrobacterium tumefaciens
Agrobacterium tumefaciense0pathogen - Second interactor
- P. fluorescens P3/pME6863Pseudomonas fluorescense0biocontrol_agent
- Outcome
- crown gall of tomato caused by Agrobacterium tumefaciense0
- Direction
- reducede0
- Comparator
- Bacillus sp. A24e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P. fluorescens P3/pME6863 significantly reduced potato soft rot caused by Erwinia carotovora and crown gall of tomato caused by Agrobacterium tumefaciens to a similar level as Bacillus sp. A24”
Claim · model-written summary
Wild type P3 with empty vector gave little or no disease reduction.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- wild-type strain P3 carrying the vector plasmid without aiiAe0
- Interactor
- wild-type strain P3e0biocontrol_agent
- Outcome
- disease reductione0
- Direction
- Little or noe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Little or no disease reduction was observed for the wild-type strain P3 carrying the vector plasmid without aiiA”
t0“potato soft rot caused by Erwinia carotovora”
Claim · model-written summary
Potato soft rot was suppressed even when the degrader was applied two days after pathogen inoculation.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- AHL-degrading P. fluorescens P3/pME6863e0
- Interactor
- P. fluorescens P3/pME6863Pseudomonas fluorescense0biocontrol_agent
- Outcome
- potato soft rote0
- Direction
- Suppressione0
- Qualifier
- 2 days after the pathogene0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Suppression of potato soft rot was observed even when the AHL-degrading P. fluorescens P3/pME6863 was applied to tubers 2 days after the pathogen”
Claim · model-written summary
AHL degraders provided greater bacterial disease control than the compared antibiotic-producing strains.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- AHL degraderse0
- Interactor
- AHL degraderse0biocontrol_agent
- Outcome
- biocontrol activitye0
- Direction
- greatere0
- Comparator
- Pseudomonas 2,4-diacetylphloroglucinol-producing strainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“biocontrol activity of the AHL degraders was greater than that observed with several Pseudomonas 2,4-diacetylphloroglucinol-producing strains and with Pseudomonas chlororaphis PCL1391”
t0“potato soft rot caused by Erwinia carotovora”
Claim · model-written summary
Co-inoculation with the AHL degrader interfered with suppression of tomato Fusarium wilt by P. chlororaphis.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- P. fluorescens P3/pME6863e0
- Interactor
- Fusarium oxysporum f. sp. lycopersici
Fusarium oxysporum f. sp. lycopersicie0pathogen - Second interactor
- P. fluorescens P3/pME6863Pseudomonas fluorescense0biocontrol_agent
- Outcome
- tomato vascular wilt caused by Fusarium oxysporum f. sp. lycopersicie0
- Direction
- interferede0
- Comparator
- P. chlororaphis PCL1391e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When P. chlororaphis PCL1391 was co-inoculated with P. fluorescens P3/pME6863 in a strain mixture, the AHL degrader interfered with the normally excellent ability of the antibiotic producer to suppress tomato vascular wilt caused by Fusarium oxysporum f. sp. lycopersici”
The same questions in other papers
Pseudomonas fluorescens · Fusarium oxysporum f. sp. lycopersici · Solanum lycopersicum (tomato)Positive and negative results
6 in the opposite direction
- positive
- positive
- positive
3 papers more in this group
See all 7 papers →Pseudomonas fluorescens · disease · Solanum tuberosum (potato)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.