Abstract
Two nitrogen-fixing root nodule symbioses between soil bacteria and higher plants have been described: the symbiosis between legume and rhizobia and actinorhizal symbioses between plants belonging to eight angiosperm families and the actinomycete Frankia. We have recently shown that the subtilisin-like Ser protease gene cg12 (isolated from the actinorhizal plant Casuarina glauca) is specifically expressed during plant cell infection by Frankia. Here we report on the study of C. glauca cg12 promoter activity in the transgenic legume Medicago truncatula. We found that cg12 promoter activation is associated with plant cell infection by Sinorhizobium meliloti. Furthermore, applications of purified Nod factors and mycorrhizal inoculation failed to trigger expression of the cg12-reporter gene construct. This indicates that at least part of the transcriptional environment in plant cells infected by endosymbiotic nitrogen-fixing bacteria is conserved between legume and actinorhizal plants. These results are discussed in view of recent data concerning molecular phylogeny that suggest a common evolutionary origin of all plants entering nitrogen-fixing root nodule symbioses.
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Claims 4
Claim · model-written summary
cg12 promoter activation was associated with plant cell infection by Sinorhizobium meliloti.
- Taxon
- Medicago truncatula
Medicago truncatulae1 - Interactor
- Sinorhizobium meliloti
Sinorhizobium melilotie0symbiont - Gene
- cg12e0
- Outcome
- cg12 promoter activatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We found that cg12 promoter activation is associated with plant cell infection by Sinorhizobium meliloti”
e1“transgenic legume Medicago truncatula”
Claim · model-written summary
Purified Nod factors failed to trigger cg12 reporter expression.
- Taxon
- Medicago truncatulaMedicago truncatulae1
- Treatment
- applications of purified Nod factorse0
- Gene
- cg12-reporter gene constructe0
- Outcome
- expression of the cg12-reporter gene constructe0
- Direction
- failed to triggere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“applications of purified Nod factors and mycorrhizal inoculation failed to trigger expression of the cg12-reporter gene construct”
e1“transgenic legume Medicago truncatula”
Claim · model-written summary
Mycorrhizal inoculation failed to trigger cg12 reporter expression.
- Taxon
- Medicago truncatulaMedicago truncatulae1
- Treatment
- mycorrhizal inoculatione0
- Gene
- cg12-reporter gene constructe0
- Outcome
- expression of the cg12-reporter gene constructe0
- Direction
- failed to triggere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“applications of purified Nod factors and mycorrhizal inoculation failed to trigger expression of the cg12-reporter gene construct”
e1“transgenic legume Medicago truncatula”
Claim · model-written summary
The authors infer conservation of the transcriptional environment in infected legume and actinorhizal plant cells.
- Taxon
- Medicago truncatulaMedicago truncatulae1
- Outcome
- transcriptional environment in plant cellse0
- Qualifier
- infected by endosymbiotic nitrogen-fixing bacteriae0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at least part of the transcriptional environment in plant cells infected by endosymbiotic nitrogen-fixing bacteria is conserved between legume and actinorhizal plants”
e1“transgenic legume Medicago truncatula”
The same questions in other papers
nodulation factor · molecular: expression · Medicago truncatula (barrel medic)Positive and negative results
4 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
4 papers more in this group
See all 8 papers →mycorrhiza · molecular: expression · Medicago truncatula (barrel medic)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
inoculation · molecular: expression · Medicago truncatula (barrel medic)Positive and negative results
1 paper in the same direction as this paper · 10 in the opposite direction
- no effect
- positive
- positive
8 papers more in this group
See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.