Abstract
This article describes an efficient procedure to study Nod factor-induced gene expression in root hairs of the model legume Medicago truncatula. By developing an improved method of fracturing frozen root hairs, it has been possible to obtain a highly purified root hair fraction from M. truncatula seedlings yielding sufficient RNA for real-time quantitative RT-PCR expression analysis. After Nod factor treatment it was possible to detect up to 100-fold increases of MtENOD11 and pMtENOD11-gus transcript levels in root hair RNA. This corresponds to 5-7-fold higher induction levels than for entire root tissue preparations. Furthermore, the use of these enriched RNA samples has revealed for the first time a very significant induction (30-fold) of the MtENOD40 gene in root hairs in response to Nod factors. It is concluded that the rapid and convenient procedure described here will be particularly useful for detecting tissue-specific low-level gene expression in root hairs responding to Rhizobium Nod factors or other exogenous signals.
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Claims 3
Claim · model-written summary
An improved method of fracturing frozen root hairs yielded a highly purified root hair fraction from M. truncatula seedlings with sufficient RNA for real-time quantitative RT-PCR.
- Taxon
- M. truncatula
Medicago truncatulae1 - Organ / stage
- root haire1
- Outcome
- highly purified root hair fractione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“By developing an improved method of fracturing frozen root hairs, it has been possible to obtain a highly purified root hair fraction from M. truncatula seedlings yielding sufficient RNA for real-time quantitative RT-PCR expression analysis”
Claim · model-written summary
After Nod factor treatment, up to 100-fold increases of MtENOD11 and pMtENOD11-gus transcript levels were detected in root hair RNA.
- Taxon
- Medicago truncatulaMedicago truncatulat0
- Organ / stage
- root haire2
- Treatment
- Nod factor treatmente2
- Gene
- MtENOD11e2
- Outcome
- transcript levelse2
- Direction
- increasese2
- Value
- up to 100-folde2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Medicago truncatula”
e2“After Nod factor treatment it was possible to detect up to 100-fold increases of MtENOD11 and pMtENOD11-gus transcript levels in root hair RNA”
Claim · model-written summary
Induction levels in root hair RNA were 5-7-fold higher than in entire root tissue preparations.
- Taxon
- Medicago truncatulaMedicago truncatulat0
- Outcome
- induction levelse3
- Direction
- highere3
- Value
- 5-7-fold highere3
- Comparator
- entire root tissue preparationse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Medicago truncatula”
e3“This corresponds to 5-7-fold higher induction levels than for entire root tissue preparations”
The same questions in other papers
nodulation factor · molecular: expression · Medicago truncatula (barrel medic)Positive and negative results
2 papers in the same direction as this paper · 5 in the opposite direction
- positive
- no effect
- negative
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.