Abstract
Abstract The in vivo nitrate reductase activity in leaf tissue of cotton ( Gossypium hirsutum L.) was characterized. Enzymatic activity was linear with time up to 60 min. The assay for nitrate reductase activity was optimized in leaf slices 400 μm wide incubated in an anaerobic system at 30°C, in a 0.02 M KNO 3 medium at pH 7.0 with 1 % propanol. In vivo activity was highest in recently matured leaves at the top of the plant. Both light and nitrate enhanced in vivo enzymatic activity. The activity was highest after 9 hours in the light and then decreased steadily for several more hours even in the presence of light. The nitrate reductase activity was more strongly correlated to the levels of NO 3 ‐N in the culture solution than to the NO 3 ‐N level in the tissue. The utility of this technique in nitrate reductase assay in a tissue containing large amounts of phenolic compounds is discussed.
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Claims 8
Claim · model-written summary
Cotton leaf nitrate reductase activity was linear with time up to 60 minutes.
- Taxon
- Cotton
Gossypium hirsutumt0 - Gene
- nitrate reductasee1
- Outcome
- Enzymatic activitye0
- Direction
- lineare0
- Value
- 60 mine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Enzymatic activity was linear with time up to 60 min.”
e1“The assay for nitrate reductase activity was optimized in leaf slices 400 μm wide incubated in an anaerobic system at 30°C, in a 0.02 M KNO 3 medium at pH 7.0 with 1 % propanol.”
t0“Cotton”
Claim · model-written summary
The assay was optimized in 400 μm leaf slices under anaerobic conditions at 30°C and specified medium conditions.
- Taxon
- CottonGossypium hirsutumt0
- Organ / stage
- leaf slicese0
- Gene
- nitrate reductasee0
- Outcome
- assay for nitrate reductase activitye0
- Value
- 400 μme0
- Qualifier
- anaerobic system at 30°C, in a 0.02 M KNO 3 medium at pH 7.0 with 1 % propanole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The assay for nitrate reductase activity was optimized in leaf slices 400 μm wide incubated in an anaerobic system at 30°C, in a 0.02 M KNO 3 medium at pH 7.0 with 1 % propanol.”
t0“Cotton”
Claim · model-written summary
In vivo activity was highest in recently matured top leaves.
- Taxon
- CottonGossypium hirsutumt0
- Organ / stage
- recently matured leavese0
- Outcome
- In vivo activitye0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vivo activity was highest in recently matured leaves at the top of the plant.”
t0“Cotton”
Claim · model-written summary
Light enhanced in vivo nitrate reductase activity.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- lighte0
- Outcome
- in vivo enzymatic activitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both light and nitrate enhanced in vivo enzymatic activity.”
t0“Cotton”
Claim · model-written summary
Nitrate enhanced in vivo nitrate reductase activity.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- nitratee0
- Outcome
- in vivo enzymatic activitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both light and nitrate enhanced in vivo enzymatic activity.”
t0“Cotton”
Claim · model-written summary
Activity peaked after nine hours of light.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activity was highest after 9 hours in the light and then decreased steadily for several more hours even in the presence of light.”
t0“Cotton”
Claim · model-written summary
After its light-associated peak, activity decreased steadily despite continued light.
- Taxon
- CottonGossypium hirsutumt0
- Outcome
- activitye0
- Direction
- decreased steadilye0
- Qualifier
- even in the presence of lighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activity was highest after 9 hours in the light and then decreased steadily for several more hours even in the presence of light.”
t0“Cotton”
Claim · model-written summary
Nitrate reductase activity correlated more strongly with solution nitrate nitrogen than tissue nitrate nitrogen.
- Taxon
- CottonGossypium hirsutumt0
- Gene
- nitrate reductasee0
- Outcome
- nitrate reductase activitye0
- Qualifier
- NO 3 ‐N in the culture solutione0
- Comparator
- NO 3 ‐N level in the tissuee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The nitrate reductase activity was more strongly correlated to the levels of NO 3 ‐N in the culture solution than to the NO 3 ‐N level in the tissue.”
t0“Cotton”
The same questions in other papers
light · physiology: enzyme activity · Gossypium hirsutum (cotton)Positive and negative results
1 in the opposite direction
- negative
nitrogen · physiology: enzyme activity · Gossypium hirsutum (cotton)Results agree
8 papers in the same direction as this paper
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.