Abstract
Experiments were conducted to determine if pretreatment of cotton (Gossypium hirsutum L.) plants resulted in differential in vitro stabilities of nitrate reductase (NR) activity. Although NR activity declines markedly during the second half of the daily light period, in vitro NR stability is not modified by time of harvest. Phenylmethylsulfonylfluoride, iodoacetamide, and N-ethylmaleimide do not influence in vitro NR stability, suggesting that serine or sulfhydryl proteases are not responsible for in vitro lability of NR from cotton cotyledons.Imposition of water stress or artificial extension of the dark period lead to significant reductions in NR activity, but do not change in vitro NR stability.Dilution of a crude extract leads to increasing lability of NR; hence the marked instability of NR cannot be attributed to an inactivator which follows simple enzyme kinetics. Since in vitro NR activity is much more stable in presence of both NADH and NO(3) (-), substrate availability must be considered as a possible factor influencing in vivo NR stability.
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Claims 9
Claim · model-written summary
NR activity declined during the second half of the daily light period.
- Taxon
- cotton
Gossypium hirsutume0 - Gene
- NRe1
- Outcome
- NR activitye1
- Direction
- declinese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cotton (Gossypium hirsutum L.) plants”
e1“NR activity declines markedly during the second half of the daily light period”
Claim · model-written summary
Harvest time did not modify in vitro NR stability.
- Taxon
- cottonGossypium hirsutume0
- Gene
- NRe1
- Outcome
- in vitro NR stabilitye1
- Direction
- not modifiede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cotton (Gossypium hirsutum L.) plants”
e1“in vitro NR stability is not modified by time of harvest”
Claim · model-written summary
Phenylmethylsulfonylfluoride, iodoacetamide, and N-ethylmaleimide did not influence in vitro NR stability.
- Taxon
- cottonGossypium hirsutume0
- Gene
- NRe1
- Outcome
- in vitro NR stabilitye1
- Direction
- do not influencee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cotton cotyledons”
e1“Phenylmethylsulfonylfluoride, iodoacetamide, and N-ethylmaleimide do not influence in vitro NR stability”
Claim · model-written summary
Water stress significantly reduced NR activity.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- water stresse1
- Outcome
- NR activitye1
- Direction
- reductionse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cotton (Gossypium hirsutum L.) plants”
e1“Imposition of water stress or artificial extension of the dark period lead to significant reductions in NR activity”
Claim · model-written summary
Artificial extension of the dark period significantly reduced NR activity.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- artificial extension of the dark periode1
- Outcome
- NR activitye1
- Direction
- reductionse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cotton (Gossypium hirsutum L.) plants”
e1“Imposition of water stress or artificial extension of the dark period lead to significant reductions in NR activity”
Claim · model-written summary
Water stress did not change in vitro NR stability.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- water stresse1
- Outcome
- in vitro NR stabilitye1
- Direction
- do not changee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cotton (Gossypium hirsutum L.) plants”
e1“Imposition of water stress or artificial extension of the dark period lead to significant reductions in NR activity, but do not change in vitro NR stability”
Claim · model-written summary
Artificial extension of the dark period did not change in vitro NR stability.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- artificial extension of the dark periode1
- Outcome
- in vitro NR stabilitye1
- Direction
- do not changee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cotton (Gossypium hirsutum L.) plants”
e1“Imposition of water stress or artificial extension of the dark period lead to significant reductions in NR activity, but do not change in vitro NR stability”
Claim · model-written summary
Diluting crude extract increased NR lability.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- Dilution of a crude extracte1
- Outcome
- lability of NRe1
- Direction
- increasinge1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cotton (Gossypium hirsutum L.) plants”
e1“Dilution of a crude extract leads to increasing lability of NR”
Claim · model-written summary
NR activity was much more stable in the presence of both NADH and nitrate.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- both NADH and NO(3) (-)e1
- Outcome
- in vitro NR activitye1
- Direction
- more stablee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cotton (Gossypium hirsutum L.) plants”
e1“in vitro NR activity is much more stable in presence of both NADH and NO(3) (-)”
The same questions in other papers
light · physiology: enzyme activity · Gossypium hirsutum (cotton)Positive and negative results
1 in the opposite direction
- positive
botanical · physiology: enzyme activity · Gossypium hirsutum (cotton)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
drought · physiology: enzyme activity · Gossypium hirsutum (cotton)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- no effect
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.