Abstract
Germinating cotton (Gossypium hirsutum L. cv. Deltapine 16) cotyledons developed two peaks of in vitro nitrate reductase activity; the first was stable in vitro and appeared 24 hours after imbibition; and the second, which was extremely labile in vitro, began to develop after the seedlings had emerged and developed chlorophyll. Nitrite reductase activity peaked only after the seedlings had emerged. Dowex 1-Cl (10%, w/v) and bovine serum albumin (3%, w/v) significantly improved the activity of extracted enzyme; greater improvement occurred as expansion of the cotyledons progressed. The major effect of bovine serum albumin on nitrate reductase activity in cotyledon extracts appeared to be that of making the extracted enzyme more active rather than increasing the amount of nitrate reductase extracted or improving the stability of the extracted enzyme.Attempts to correlate protease activity with the increasingly labile nitrate reductase activity in expanding cotyledons were unsuccessful. Instead, when extracts containing stable nitrate reductase were mixed with extracts containing labile nitrate reductase, the latter was stabilized. The nature of the "protector" in the stable extracts is not known. It is heat-stable, but apparently does not function in vivo since nitrate reductase in germinating cotton seedlings rapidly declines following a peak of activity at 24 hours. We suggest that the protector may function by preventing nitrate reductase from dissociating into inactive subunits.
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Claims 12
Claim · model-written summary
A first stable nitrate reductase peak appeared 24 hours after imbibition.
- Taxon
- cotton
Gossypium hirsutumt0 - Outcome
- first was stable in vitroe0
- Value
- 24 hourse0
Not reportedOrgan / stage
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the first was stable in vitro and appeared 24 hours after imbibition”
t0“Cotton Cotyledons”
Claim · model-written summary
A second labile nitrate reductase peak developed after emergence and chlorophyll development.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- seedlingse0
- Outcome
- second, which was extremely labile in vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the second, which was extremely labile in vitro, began to develop after the seedlings had emerged and developed chlorophyll”
t0“Cotton Cotyledons”
Claim · model-written summary
Nitrite reductase activity peaked only after emergence.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- seedlingse0
- Outcome
- Nitrite reductase activity peakede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nitrite reductase activity peaked only after the seedlings had emerged”
t0“Cotton Cotyledons”
Claim · model-written summary
Dowex improved extracted enzyme activity.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- Dowex 1-Cl (10%, w/v)e0
- Outcome
- activity of extracted enzymee0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Dowex 1-Cl (10%, w/v) and bovine serum albumin (3%, w/v) significantly improved the activity of extracted enzyme”
t0“Cotton Cotyledons”
Claim · model-written summary
Bovine serum albumin improved extracted enzyme activity.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- bovine serum albumin (3%, w/v)e0
- Outcome
- activity of extracted enzymee0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Dowex 1-Cl (10%, w/v) and bovine serum albumin (3%, w/v) significantly improved the activity of extracted enzyme”
t0“Cotton Cotyledons”
Claim · model-written summary
The improvement grew as cotyledons expanded.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- cotyledonse0
- Outcome
- improvemente0
- Direction
- greatere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“greater improvement occurred as expansion of the cotyledons progressed”
t0“Cotton Cotyledons”
Claim · model-written summary
Albumin appeared to activate extracted nitrate reductase.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- bovine serum albumine0
- Gene
- nitrate reductasee0
- Outcome
- nitrate reductase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The major effect of bovine serum albumin on nitrate reductase activity in cotyledon extracts appeared to be that of making the extracted enzyme more active”
t0“Cotton Cotyledons”
Claim · model-written summary
Protease activity did not correlate with nitrate reductase lability.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- cotyledonse0
- Gene
- proteasee0
- Outcome
- nitrate reductase activitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Attempts to correlate protease activity with the increasingly labile nitrate reductase activity in expanding cotyledons were unsuccessful”
t0“Cotton Cotyledons”
Claim · model-written summary
Stable extracts stabilized labile nitrate reductase when mixed.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- extracts containing stable nitrate reductasee0
- Outcome
- labile nitrate reductasee0
- Direction
- stabilizede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when extracts containing stable nitrate reductase were mixed with extracts containing labile nitrate reductase, the latter was stabilized”
t0“Cotton Cotyledons”
Claim · model-written summary
The protector was heat-stable.
- Taxon
- cottonGossypium hirsutumt0
- Outcome
- heat-stablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It is heat-stable”
t0“Cotton Cotyledons”
Claim · model-written summary
Nitrate reductase rapidly declined after its 24-hour peak.
- Taxon
- cottonGossypium hirsutume0
- Organ / stage
- seedlingse0
- Outcome
- nitrate reductasee0
- Direction
- declinese0
- Value
- 24 hourse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nitrate reductase in germinating cotton seedlings rapidly declines following a peak of activity at 24 hours”
Claim · model-written summary
Authors suggest the protector may prevent inactive subunit dissociation.
- Taxon
- cottonGossypium hirsutumt0
- Outcome
- nitrate reductase from dissociating into inactive subunitse0
- Qualifier
- the protectore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the protector may function by preventing nitrate reductase from dissociating into inactive subunits”
t0“Cotton Cotyledons”
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See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.