Abstract
The MtENOD11 gene from the model legume Medicago truncatula is transcriptionally activated both in response to Sinorhizobium meliloti Nod factors and throughout infection of root tissues by the nitrogen-fixing microsymbiont. To identify the regulatory sequences involved in symbiosis-related MtENOD11 expression, a series of promoter deletions driving the beta-glucuronidase reporter gene were analyzed in transgenic M. truncatula roots. These studies have revealed that distinct regulatory regions are involved in infection-related MtENOD11 expression compared with preinfection (Nod factor-mediated) expression. In particular, the 257-bp promoter sequence immediately upstream from the start codon is sufficient for infection-related expression, but is unable to drive gene transcription in response to the Nod factor elicitor. This truncated promoter is also sufficient to confer MtENOD11 expression during both the arbuscular mycorrhizal (AM) association and the parasitic interaction with root-knot nematodes. Site-directed mutagenesis further showed that a previously identified nodule-specific AT-rich motif is required for high-level MtENOD11 expression during S. meliloti infection as well as during AM colonization. However, mutation of this motif does not affect gene expression associated with nematode-feeding sites. Taken together, these results suggest a close link between regulatory mechanisms controlling transcriptional early nodulin gene activation during both rhizobial and AM root endosymbioses.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Infection-related MtENOD11 expression used regulatory regions distinct from Nod factor expression.
- Taxon
- Medicago truncatula
Medicago truncatulae1 - Gene
- MtENOD11e0
- Outcome
- infection-related MtENOD11 expressione0
- Comparator
- preinfection (Nod factor-mediated) expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“distinct regulatory regions are involved in infection-related MtENOD11 expression compared with preinfection (Nod factor-mediated) expression.”
t0“MtENOD11 Gene Activation”
e1“The MtENOD11 gene from the model legume Medicago truncatula is transcriptionally activated both in response to Sinorhizobium meliloti Nod factors and throughout infection of root tissues by the nitrogen-fixing microsymbiont.”
Claim · model-written summary
The 257-bp upstream promoter was sufficient for infection-related expression.
- Taxon
- Medicago truncatulaMedicago truncatulae1
- Gene
- MtENOD11t0
- Outcome
- infection-related expressione0
- Value
- 257-bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the 257-bp promoter sequence immediately upstream from the start codon is sufficient for infection-related expression”
t0“MtENOD11 Gene Activation”
e1“The MtENOD11 gene from the model legume Medicago truncatula is transcriptionally activated both in response to Sinorhizobium meliloti Nod factors and throughout infection of root tissues by the nitrogen-fixing microsymbiont.”
Claim · model-written summary
The truncated promoter did not drive transcription following Nod factor elicitation.
- Taxon
- Medicago truncatulaMedicago truncatulae1
- Treatment
- Nod factor elicitore0
- Gene
- MtENOD11t0
- Outcome
- gene transcriptione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but is unable to drive gene transcription in response to the Nod factor elicitor.”
t0“MtENOD11 Gene Activation”
e1“The MtENOD11 gene from the model legume Medicago truncatula is transcriptionally activated both in response to Sinorhizobium meliloti Nod factors and throughout infection of root tissues by the nitrogen-fixing microsymbiont.”
Claim · model-written summary
The truncated promoter conferred MtENOD11 expression during AM association.
- Taxon
- Medicago truncatulaMedicago truncatulae1
- Gene
- MtENOD11e0
- Outcome
- MtENOD11 expressione0
- Comparator
- arbuscular mycorrhizal (AM) associatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This truncated promoter is also sufficient to confer MtENOD11 expression during both the arbuscular mycorrhizal (AM) association and the parasitic interaction with root-knot nematodes.”
t0“MtENOD11 Gene Activation”
e1“The MtENOD11 gene from the model legume Medicago truncatula is transcriptionally activated both in response to Sinorhizobium meliloti Nod factors and throughout infection of root tissues by the nitrogen-fixing microsymbiont.”
Claim · model-written summary
The truncated promoter conferred MtENOD11 expression during nematode interaction.
- Taxon
- Medicago truncatulaMedicago truncatulae1
- Interactor
- root-knot nematodes
_penetrates_a_tomato_root_-_USDA-ARS.jpg?width=60)
Meloidogynee0pest - Gene
- MtENOD11e0
- Outcome
- MtENOD11 expressione0
- Comparator
- root-knot nematodese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_penetrates_a_tomato_root_-_USDA-ARS.jpg?width=500)
Verbatim
e0“This truncated promoter is also sufficient to confer MtENOD11 expression during both the arbuscular mycorrhizal (AM) association and the parasitic interaction with root-knot nematodes.”
t0“MtENOD11 Gene Activation”
e1“The MtENOD11 gene from the model legume Medicago truncatula is transcriptionally activated both in response to Sinorhizobium meliloti Nod factors and throughout infection of root tissues by the nitrogen-fixing microsymbiont.”
Claim · model-written summary
The AT-rich motif was required for high MtENOD11 expression during S. meliloti infection.
- Taxon
- Medicago truncatulaMedicago truncatulae1
- Interactor
- S. meliloti
Sinorhizobium melilotie0symbiont - Gene
- AT-rich motife0
- Outcome
- MtENOD11 expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a previously identified nodule-specific AT-rich motif is required for high-level MtENOD11 expression during S. meliloti infection”
t0“MtENOD11 Gene Activation”
e1“The MtENOD11 gene from the model legume Medicago truncatula is transcriptionally activated both in response to Sinorhizobium meliloti Nod factors and throughout infection of root tissues by the nitrogen-fixing microsymbiont.”
Claim · model-written summary
The AT-rich motif was required for high MtENOD11 expression during AM colonization.
- Taxon
- Medicago truncatulaMedicago truncatulae1
- Interactor
- AMe0symbiont
- Gene
- AT-rich motife0
- Outcome
- MtENOD11 expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a previously identified nodule-specific AT-rich motif is required for high-level MtENOD11 expression during S. meliloti infection as well as during AM colonization.”
t0“MtENOD11 Gene Activation”
e1“The MtENOD11 gene from the model legume Medicago truncatula is transcriptionally activated both in response to Sinorhizobium meliloti Nod factors and throughout infection of root tissues by the nitrogen-fixing microsymbiont.”
Claim · model-written summary
AT-rich motif mutation did not affect expression at nematode feeding sites.
- Taxon
- Medicago truncatulaMedicago truncatulae1
- Treatment
- mutation of this motife0
- Outcome
- gene expression associated with nematode-feeding sitese0
- Direction
- does not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutation of this motif does not affect gene expression associated with nematode-feeding sites.”
t0“MtENOD11 Gene Activation”
e1“The MtENOD11 gene from the model legume Medicago truncatula is transcriptionally activated both in response to Sinorhizobium meliloti Nod factors and throughout infection of root tissues by the nitrogen-fixing microsymbiont.”
The same questions in other papers
nodulation factor · molecular: expression · Medicago truncatula (barrel medic)Positive and negative results
4 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
4 papers more in this group
See all 8 papers →resistance inducer · molecular: expression · Medicago truncatula (barrel medic)Positive and negative results
1 in the opposite direction
- positive
loss of function · molecular: expression · Medicago truncatula (barrel medic)Results agree
7 papers in the same direction as this paper
- negative
- negative
- negative
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.