Abstract
Enhanced disease susceptibility1 (EDS1) is a key node in the plant immune signaling network, regulating salicylic acid (SA) levels and other immune responses in Arabidopsis thaliana. We previously reported that modulation of SA by AGD2-like defense response protein 1 (ALD1) has been shown to influence the immune response in Lotus japonicus, but the role of LjEDS1 in this species remains unclear. Here, we identified and characterized the LjEDS1 gene in L. japonicus. The LjEDS1 protein contains a lipase-like domain and an EP domain similar to the Arabidopsis EDS1 protein. Subcellular localization studies revealed that the LjEDS1 protein is distributed in both the cytoplasm and nucleus. Heterologous expression of LjEDS1 in the Arabidopsis ateds1 mutant increased resistance to Pseudomonas syringae pv. Tomato (Pst) strain DC3000. In L. japonicus, roots of the ljeds1 mutants exhibited heightened susceptibility to Ralstonia solanacearum, with increased lesion areas and bacterial titers. Conversely, the overexpression of LjEDS1 reduced the lesion areas and bacterial titers in roots infected with R. solanacearum compared to those in the wild-type. Gene expression analysis showed that LjEDS1 regulates defense-related, basal immunity, and oxidative stress response genes in L. japonicus roots. These findings establish LjEDS1 as an important regulator of disease resistance in L. japonicus.
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Claims 8
Claim · model-written summary
LjEDS1 protein contains lipase-like and EP domains.
- Taxon
- Lotus japonicus
Lotus japonicust0 - Gene
- LjEDS1e0
- Outcome
- lipase-like domain and an EP domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The LjEDS1 protein contains a lipase-like domain and an EP domain similar to the Arabidopsis EDS1 protein”
t0“Lotus japonicus”
Claim · model-written summary
LjEDS1 localizes to cytoplasm and nucleus.
- Taxon
- Lotus japonicusLotus japonicust0
- Gene
- LjEDS1e0
- Outcome
- distributed in both the cytoplasm and nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LjEDS1 protein is distributed in both the cytoplasm and nucleus”
t0“Lotus japonicus”
Claim · model-written summary
LjEDS1 expression raised Arabidopsis resistance to Pseudomonas syringae DC3000.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- Heterologous expression of LjEDS1e0
- Interactor
- Pseudomonas syringae pv. Tomato (Pst) strain DC3000
Pseudomonas syringaee0pathogen - Gene
- LjEDS1e0
- Outcome
- resistance to Pseudomonas syringae pv. Tomato (Pst) strain DC3000e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Heterologous expression of LjEDS1 in the Arabidopsis ateds1 mutant increased resistance to Pseudomonas syringae pv. Tomato (Pst) strain DC3000”
Claim · model-written summary
Lotus ljeds1 mutant roots had increased lesion areas under Ralstonia infection.
- Taxon
- Lotus japonicusLotus japonicust0
- Material
- ljeds1 mutantse0
- Interactor
- Ralstonia solanacearum
Ralstonia solanacearume0pathogen - Outcome
- lesion arease0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“roots of the ljeds1 mutants exhibited heightened susceptibility to Ralstonia solanacearum, with increased lesion areas and bacterial titers”
t0“Lotus japonicus”
Claim · model-written summary
Lotus ljeds1 mutant roots had increased Ralstonia titers.
- Taxon
- Lotus japonicusLotus japonicust0
- Material
- ljeds1 mutantse0
- Interactor
- Ralstonia solanacearumRalstonia solanacearume0pathogen
- Outcome
- bacterial titerse0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“roots of the ljeds1 mutants exhibited heightened susceptibility to Ralstonia solanacearum, with increased lesion areas and bacterial titers”
t0“Lotus japonicus”
Claim · model-written summary
LjEDS1 overexpression reduced lesion areas versus wild type under Ralstonia infection.
- Taxon
- Lotus japonicusLotus japonicust0
- Treatment
- overexpression of LjEDS1e0
- Interactor
- R. solanacearumRalstonia solanacearume0pathogen
- Outcome
- lesion arease0
- Direction
- reducede0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of LjEDS1 reduced the lesion areas and bacterial titers in roots infected with R. solanacearum compared to those in the wild-type”
t0“Lotus japonicus”
Claim · model-written summary
LjEDS1 overexpression reduced bacterial titers versus wild type.
- Taxon
- Lotus japonicusLotus japonicust0
- Treatment
- overexpression of LjEDS1e0
- Interactor
- R. solanacearumRalstonia solanacearume0pathogen
- Outcome
- bacterial titerse0
- Direction
- reducede0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of LjEDS1 reduced the lesion areas and bacterial titers in roots infected with R. solanacearum compared to those in the wild-type”
t0“Lotus japonicus”
Claim · model-written summary
LjEDS1 regulates defense, basal immunity and oxidative stress response genes in Lotus roots.
- Taxon
- L. japonicusLotus japonicuse0
- Gene
- LjEDS1e0
- Outcome
- defense-related, basal immunity, and oxidative stress response genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LjEDS1 regulates defense-related, basal immunity, and oxidative stress response genes in L. japonicus roots”
The same questions in other papers
overexpression · Pseudomonas syringae · ArabidopsisPositive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- negative
- no effect
- positive
1 paper more in this group
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