Abstract
In vitro -grown shoot tips of five tetraploid potato ( Solanum tuberosum L.) clones were cryopreserved by vitrification. Excised shoot tips (0.5–0.7 mm) were pre-cultured on filter paper discs over half strength liquid Murashige and Skoog (MS) medium supplemented with 8.7 μ m GA 3 and different combinations of sucrose (0.3, 0.5 and 0.7 m ) plus mannitol (0, 0.2 and 0.4 m ) for 2 d under a 16 h photoperiod at 24 °C. The pre-cultured shoot tips were either successively loaded with 20 and 60% PVS 2 solutions or directly exposed to concentrated vitrification solution before physical vitrification during liquid nitrogen treatment. The vitrified shoot tips were warmed rapidly and treated with dilution mixture (MS+1.2 m sucrose) for 30 min before plating on regrowth medium. Addition of mannitol to the pre-culture medium improved survival of vitrified shoot tips. Direct dehydration of pre-cultured shoot tips with concentrated PVS 2 was detrimental to survival of vitrified shoot tips. Shoot tips pre-cultured on medium containing 0.3 m sucrose plus 0.2 m mannitol, and loaded with 20% PVS 2 for 30 min followed by 15 min incubation in 60% PVS 2 and 5 min incubation in 100% PVS 2 at 0 °C resulted in up to 54% survival after vitrification. About 50% of vitrified and warmed shoot tips formed shoots directly. Post-thaw culturing of vitrified shoot tips on medium containing an elevated level of sucrose (0.2 m ) under diffuse light for the first week enhanced the survival rate. Continuous culturing of vitrified shoot tips on high-sucrose medium induced multiple shoot formation. Copyright 1998 Annals of Botany Company
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Claims 6
Claim · model-written summary
Mannitol in preculture medium improved survival of vitrified shoot tips.
- Taxon
- Potato
Solanum tuberosumt0 - Treatment
- Addition of mannitol to the pre-culture mediume0
- Outcome
- survival of vitrified shoot tipse0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato”
e0“Addition of mannitol to the pre-culture medium improved survival of vitrified shoot tips.”
Claim · model-written summary
Direct dehydration with concentrated PVS 2 harmed shoot-tip survival.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- Direct dehydration of pre-cultured shoot tips with concentrated PVS 2e0
- Outcome
- survival of vitrified shoot tipse0
- Direction
- detrimentale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato”
e0“Direct dehydration of pre-cultured shoot tips with concentrated PVS 2 was detrimental to survival of vitrified shoot tips.”
Claim · model-written summary
The specified sucrose, mannitol and PVS 2 sequence yielded up to 54% shoot-tip survival.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- 0.3 m sucrose plus 0.2 m mannitole0
- Outcome
- survival after vitrificatione0
- Value
- up to 54%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato”
e0“Shoot tips pre-cultured on medium containing 0.3 m sucrose plus 0.2 m mannitol, and loaded with 20% PVS 2 for 30 min followed by 15 min incubation in 60% PVS 2 and 5 min incubation in 100% PVS 2 at 0 °C resulted in up to 54% survival after vitrification.”
Claim · model-written summary
About 50% of vitrified and warmed shoot tips formed shoots directly.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- formed shoots directlye0
- Value
- About 50%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato”
e0“About 50% of vitrified and warmed shoot tips formed shoots directly.”
Claim · model-written summary
Elevated sucrose under diffuse light in the first recovery week enhanced survival.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- an elevated level of sucrose (0.2 m ) under diffuse light for the first weeke0
- Outcome
- survival ratee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato”
e0“Post-thaw culturing of vitrified shoot tips on medium containing an elevated level of sucrose (0.2 m ) under diffuse light for the first week enhanced the survival rate.”
Claim · model-written summary
Continuous culture on high-sucrose medium induced multiple shoots.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- high-sucrose mediume0
- Outcome
- multiple shoot formatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato”
e0“Continuous culturing of vitrified shoot tips on high-sucrose medium induced multiple shoot formation.”
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2 papers in the same direction as this paper
- positive
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.