Abstract
The potential of cassava starch as an alternative and cheap gelling agent for potato in vitro culture micro-propagation media was investigated. A two-factor experiment in randomized complete block design was conducted. Four levels of gelling agents; 10% (w/v) cassava starch, 8% cassava starch mixed with 0.25% agar, 0.8% agar and a liquid medium, were evaluated using three selected Kenyan potato cultivars (Tigoni, Asante and Kenya Sifa). Cassava starch at 10% gave adequate support of explants, though it had low viscosity and softened at 42 days after explant inoculation. Cassava starch mixed with 0.25% agar provided the same firmness as 0.8% agar and maintained gel integrity throughout the culturing period of 84 days. Survival in- and ex vitro was lowest in liquid medium culture. Potato transplants from the liquid medium and cassava starch gelled medium had similar (p > 0.05) mean number of nodes and biomass. These mean values were significantly higher compared to the transplants from the agar gelled medium. The use of 10% cassava starch reduced cost by 42.5% in comparison with use of agar.
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Claims 9
Claim · model-written summary
A 10% cassava starch gelling agent reduced cost by 42.5% compared with agar.
- Taxon
- potato
Solanum tuberosumt0 - Treatment
- 10% cassava starche6
- Outcome
- coste6
- Direction
- reducede6
- Value
- 42.5%e6
- Comparator
- agare6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“The use of 10% cassava starch reduced cost by 42.5% in comparison with use of agar”
t0“Cassava starch as an alternative cheap gelling agent for the in vitro micro-propagation of potato (Solanum tuberosum L.)”
Claim · model-written summary
Cassava starch mixed with 0.25% agar gave the same firmness as 0.8% agar.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- Cassava starch mixed with 0.25% agare2
- Outcome
- firmnesse2
- Direction
- samee2
- Comparator
- 0.8% agare2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Cassava starch mixed with 0.25% agar provided the same firmness as 0.8% agar and maintained gel integrity throughout the culturing period of 84 days”
t0“Cassava starch as an alternative cheap gelling agent for the in vitro micro-propagation of potato (Solanum tuberosum L.)”
Claim · model-written summary
Cassava starch mixed with 0.25% agar maintained gel integrity for 84 days of culture.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- Cassava starch mixed with 0.25% agare2
- Outcome
- gel integritye2
- Direction
- maintainede2
- Qualifier
- throughout the culturing period of 84 dayse2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Cassava starch mixed with 0.25% agar provided the same firmness as 0.8% agar and maintained gel integrity throughout the culturing period of 84 days”
t0“Cassava starch as an alternative cheap gelling agent for the in vitro micro-propagation of potato (Solanum tuberosum L.)”
Claim · model-written summary
10% cassava starch gave adequate support of potato explants.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- Cassava starch at 10%e1
- Outcome
- support of explantse1
- Qualifier
- adequatee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Cassava starch at 10% gave adequate support of explants, though it had low viscosity and softened at 42 days after explant inoculation”
t0“Cassava starch as an alternative cheap gelling agent for the in vitro micro-propagation of potato (Solanum tuberosum L.)”
Claim · model-written summary
10% cassava starch softened at 42 days after explant inoculation.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- Cassava starch at 10%e1
- Outcome
- softenede1
- Qualifier
- at 42 days after explant inoculatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Cassava starch at 10% gave adequate support of explants, though it had low viscosity and softened at 42 days after explant inoculation”
t0“Cassava starch as an alternative cheap gelling agent for the in vitro micro-propagation of potato (Solanum tuberosum L.)”
Claim · model-written summary
10% cassava starch had low viscosity.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- Cassava starch at 10%e1
- Outcome
- viscositye1
- Qualifier
- low viscositye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Cassava starch at 10% gave adequate support of explants, though it had low viscosity and softened at 42 days after explant inoculation”
t0“Cassava starch as an alternative cheap gelling agent for the in vitro micro-propagation of potato (Solanum tuberosum L.)”
Claim · model-written summary
Survival in and ex vitro was lowest in liquid medium culture.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- liquid medium culturee3
- Outcome
- Survival in- and ex vitroe3
- Direction
- loweste3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Survival in- and ex vitro was lowest in liquid medium culture”
t0“Cassava starch as an alternative cheap gelling agent for the in vitro micro-propagation of potato (Solanum tuberosum L.)”
Claim · model-written summary
Transplants from liquid medium and cassava starch gelled medium had similar numbers of nodes and biomass.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- cassava starch gelled mediume4
- Outcome
- mean number of nodes and biomasse4
- Direction
- similare4
- Comparator
- liquid mediume4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Potato transplants from the liquid medium and cassava starch gelled medium had similar (p > 0.05) mean number of nodes and biomass”
t0“Cassava starch as an alternative cheap gelling agent for the in vitro micro-propagation of potato (Solanum tuberosum L.)”
Claim · model-written summary
Transplants from cassava starch gelled medium had significantly higher numbers of nodes and biomass than those from agar gelled medium.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- cassava starch gelled mediume4
- Outcome
- mean number of nodes and biomasse4
- Direction
- significantly highere5
- Comparator
- agar gelled mediume5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Potato transplants from the liquid medium and cassava starch gelled medium had similar (p > 0.05) mean number of nodes and biomass”
e5“These mean values were significantly higher compared to the transplants from the agar gelled medium”
t0“Cassava starch as an alternative cheap gelling agent for the in vitro micro-propagation of potato (Solanum tuberosum L.)”
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