Paper · 2008 · AFRICAN JOURNAL OF BIOTECHNOLOGY

Table sugar as an alternative low cost medium component for in vitro micro-propagation of potato (Solanum tuberosum L.)

P. Demo; Paul Kuria; E. M. Kahangi

11
claims
30
verbatim quotes
34
citations
2
research areas
✓
Open access

Abstract

Most developing countries are limited in maximizing tissue culture technology due to the overhead costs involved. In view of this, the aim of this research was to evaluate alternative cheap sources of carbon and energy in potato culture media in order to reduce the overall cost of micro-propagation. A randomized complete block design was used to compare laboratory grade sucrose with two types of local commercial table sugar, specifically white and brown sugar. Three selected Kenyan potato cultivars, Tigoni, Asante and Kenya Sifa were cultured on full strength Murashige and Skoog (MS) medium at 3% (w/v) in combination with the 3 different sugars. The variation in growth performance of the cultivars was then observed. Plantlet survival of 100% was recorded after four subculture generations on all sugars for all the cultivars. The mean number of nodes per plantlet was significantly higher in brown sugar for cultivars Kenya Sifa and Asante. Brown sugar enhanced significantly higher mean number of roots per plantlet after four subculture generations for all cultivars. There was no significant difference in percentage of plantlet survival after transplanting for cultivars Asante and Kenya Sifa but significantly lower for cultivar Tigoni on grade sucrose medium. Results also showed that table sugar not only enhanced micro-propagation but also significantly lowered the production input costs by 34 to 51% when compared with the analytical grade sucrose.

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 11

Observationobservedoriginal resultmeasured

Claim · model-written summary

All cultivars had 100% plantlet survival after four subcultures on all sugars.

Outcome
Plantlet survivale0
Value
100%e0
Qualifier
after four subculture generations on all sugars for all the cultivarse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Plantlet survival of 100% was recorded after four subculture generations on all sugars for all the cultivars”

✓ located verbatim in the source · abstract, characters 726–834 · show in text ↑
t0

“potato (Solanum tuberosum L.)”

✓ located verbatim in the source · title, characters 90–119 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Brown sugar gave significantly more nodes per plantlet for Kenya Sifa.

Material
Kenya Sifae0
Treatment
brown sugare0
Outcome
mean number of nodes per plantlete0
Direction
highere0
Comparator
laboratory grade sucrosee1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The mean number of nodes per plantlet was significantly higher in brown sugar for cultivars Kenya Sifa and Asante”

✓ located verbatim in the source · abstract, characters 836–949 · show in text ↑
e1

“compare laboratory grade sucrose with two types of local commercial table sugar”

✓ located verbatim in the source · abstract, characters 348–427 · show in text ↑
t0

“potato (Solanum tuberosum L.)”

✓ located verbatim in the source · title, characters 90–119 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Brown sugar gave significantly more nodes per plantlet for Asante.

Material
Asantee0
Treatment
brown sugare0
Outcome
mean number of nodes per plantlete0
Direction
highere0
Comparator
laboratory grade sucrosee1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The mean number of nodes per plantlet was significantly higher in brown sugar for cultivars Kenya Sifa and Asante”

✓ located verbatim in the source · abstract, characters 836–949 · show in text ↑
e1

“compare laboratory grade sucrose with two types of local commercial table sugar”

✓ located verbatim in the source · abstract, characters 348–427 · show in text ↑
t0

“potato (Solanum tuberosum L.)”

✓ located verbatim in the source · title, characters 90–119 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Brown sugar significantly increased roots per plantlet for Tigoni.

Material
Tigonie1
Treatment
Brown sugare0
Outcome
mean number of roots per plantlete0
Direction
highere0
Comparator
laboratory grade sucrosee2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Brown sugar enhanced significantly higher mean number of roots per plantlet after four subculture generations for all cultivars”

✓ located verbatim in the source · abstract, characters 951–1078 · show in text ↑
e1

“Three selected Kenyan potato cultivars, Tigoni, Asante and Kenya Sifa”

✓ located verbatim in the source · abstract, characters 465–534 · show in text ↑
e2

“compare laboratory grade sucrose with two types of local commercial table sugar”

✓ located verbatim in the source · abstract, characters 348–427 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Brown sugar significantly increased roots per plantlet for Asante.

Material
Asantee1
Treatment
Brown sugare0
Outcome
mean number of roots per plantlete0
Direction
highere0
Comparator
laboratory grade sucrosee2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Brown sugar enhanced significantly higher mean number of roots per plantlet after four subculture generations for all cultivars”

✓ located verbatim in the source · abstract, characters 951–1078 · show in text ↑
e1

“Three selected Kenyan potato cultivars, Tigoni, Asante and Kenya Sifa”

✓ located verbatim in the source · abstract, characters 465–534 · show in text ↑
e2

“compare laboratory grade sucrose with two types of local commercial table sugar”

✓ located verbatim in the source · abstract, characters 348–427 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Brown sugar significantly increased roots per plantlet for Kenya Sifa.

Material
Kenya Sifae1
Treatment
Brown sugare0
Outcome
mean number of roots per plantlete0
Direction
highere0
Comparator
laboratory grade sucrosee2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Brown sugar enhanced significantly higher mean number of roots per plantlet after four subculture generations for all cultivars”

✓ located verbatim in the source · abstract, characters 951–1078 · show in text ↑
e1

“Three selected Kenyan potato cultivars, Tigoni, Asante and Kenya Sifa”

✓ located verbatim in the source · abstract, characters 465–534 · show in text ↑
e2

“compare laboratory grade sucrose with two types of local commercial table sugar”

✓ located verbatim in the source · abstract, characters 348–427 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Transplant survival did not differ significantly for Asante.

Material
Asantee0
Treatment
table sugare1
Outcome
percentage of plantlet survival after transplantinge0
Direction
no significant differencee0
Comparator
laboratory grade sucrosee1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“There was no significant difference in percentage of plantlet survival after transplanting for cultivars Asante and Kenya Sifa”

✓ located verbatim in the source · abstract, characters 1080–1206 · show in text ↑
e1

“compare laboratory grade sucrose with two types of local commercial table sugar”

✓ located verbatim in the source · abstract, characters 348–427 · show in text ↑
t0

“potato (Solanum tuberosum L.)”

✓ located verbatim in the source · title, characters 90–119 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Transplant survival did not differ significantly for Kenya Sifa.

Material
Kenya Sifae0
Treatment
table sugare1
Outcome
percentage of plantlet survival after transplantinge0
Direction
no significant differencee0
Comparator
laboratory grade sucrosee1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“There was no significant difference in percentage of plantlet survival after transplanting for cultivars Asante and Kenya Sifa”

✓ located verbatim in the source · abstract, characters 1080–1206 · show in text ↑
e1

“compare laboratory grade sucrose with two types of local commercial table sugar”

✓ located verbatim in the source · abstract, characters 348–427 · show in text ↑
t0

“potato (Solanum tuberosum L.)”

✓ located verbatim in the source · title, characters 90–119 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Tigoni transplant survival was significantly lower on grade sucrose medium.

Material
Tigonie0
Treatment
grade sucrose mediume0
Outcome
percentage of plantlet survival after transplantinge1
Direction
lowere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“significantly lower for cultivar Tigoni on grade sucrose medium”

✓ located verbatim in the source · abstract, characters 1211–1274 · show in text ↑
e1

“percentage of plantlet survival after transplanting”

✓ located verbatim in the source · abstract, characters 1119–1170 · show in text ↑
t0

“potato (Solanum tuberosum L.)”

✓ located verbatim in the source · title, characters 90–119 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Table sugar significantly reduced input costs by 34 to 51% compared with analytical-grade sucrose.

Treatment
table sugare0
Outcome
production input costse0
Direction
lowerede0
Value
34 to 51%e0
Comparator
analytical grade sucrosee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“table sugar not only enhanced micro-propagation but also significantly lowered the production input costs by 34 to 51% when compared with the analytical grade sucrose”

✓ located verbatim in the source · abstract, characters 1301–1467 · show in text ↑
t0

“potato (Solanum tuberosum L.)”

✓ located verbatim in the source · title, characters 90–119 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Table sugar enhanced potato micropropagation.

Treatment
table sugare0
Outcome
micro-propagatione0
Direction
enhancede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“table sugar not only enhanced micro-propagation”

✓ located verbatim in the source · abstract, characters 1301–1348 · show in text ↑
t0

“potato (Solanum tuberosum L.)”

✓ located verbatim in the source · title, characters 90–119 · show in text ↑

sucrose · survival · Solanum tuberosum (potato)Positive and negative results

4 papers: 2 positive, 2 negative or no effect

1 paper in the same direction as this paper · 2 in the opposite direction

  1. positive
  2. positive
  3. no effect
See all 4 papers →

sucrose · yield · Solanum tuberosum (potato)Positive and negative results

8 papers: 6 positive, 2 negative or no effect

5 papers in the same direction as this paper · 1 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. no effect
  3. positive

4 papers more in this group

See all 8 papers →

in vitro · survival · Solanum tuberosum (potato)Positive and negative results

6 papers: 4 positive, 2 negative or no effect

1 paper in the same direction as this paper · 4 in the opposite direction

  1. positive
  2. positive
  3. positive

2 papers more in this group

See all 6 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.