Abstract
Synthesis of lipidic components in anthers, including of the pollen exine, is essential for plant male reproductive development. Plant lipid transfer proteins (LTPs) are small, abundant lipid-binding proteins that have the ability to exchange lipids between membranes in vitro. However, their biological role in male reproductive development remains less understood. Here, we report the crucial role of OsC6 in regulating postmeiotic anther development in rice (Oryza sativa). Found in monocots, OsC6 belongs to a distinct clade from previously identified LTP1 and LTP2 family members found in both dicots and monocots. OsC6 expression is mainly detectable in tapetal cells and weakly in microspores from stage 9 to stage 11 of anther development. Immunological assays indicated that OsC6 is widely distributed in anther tissues such as the tapetal cytoplasm, the extracellular space between the tapetum and middle layer, and the anther locule and anther cuticle. Biochemical assays indicated that recombinant OsC6 has lipid binding activity. Moreover, plants in which OsC6 was silenced had defective development of orbicules (i.e. Ubisch bodies) and pollen exine and had reduced pollen fertility. Furthermore, additional evidence is provided that the expression of OsC6 is positively regulated by a basic helix-loop-helix transcription factor, Tapetum Degeneration Retardation (TDR). Extra granule-like structures were observed on the inner surface of the tdr tapetal layer when the expression of OsC6 was driven by the TDR promoter compared with the tdr mutant. These data suggest that OsC6 plays a crucial role in the development of lipidic orbicules and pollen exine during anther development in rice.
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Claims 8
Claim · model-written summary
OsC6 belongs to a distinct monocot clade from LTP1 and LTP2.
- Taxon
- rice
Oryza sativat0 - Gene
- OsC6e0
- Outcome
- distinct cladee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Found in monocots, OsC6 belongs to a distinct clade from previously identified LTP1 and LTP2 family members found in both dicots and monocots”
t0“Rice”
Claim · model-written summary
OsC6 expression was mainly detected in tapetal cells and weakly in microspores at stages 9 to 11.
- Taxon
- riceOryza sativat0
- Organ / stage
- tapetal cellse0
- Gene
- OsC6e0
- Outcome
- expressione0
- Qualifier
- stage 9 to stage 11 of anther developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsC6 expression is mainly detectable in tapetal cells and weakly in microspores from stage 9 to stage 11 of anther development”
t0“Rice”
Claim · model-written summary
OsC6 was distributed across tapetal cytoplasm, extracellular space, anther locule, and cuticle.
- Taxon
- riceOryza sativat0
- Gene
- OsC6e0
- Outcome
- widely distributed in anther tissuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsC6 is widely distributed in anther tissues such as the tapetal cytoplasm, the extracellular space between the tapetum and middle layer, and the anther locule and anther cuticle”
t0“Rice”
Claim · model-written summary
Recombinant OsC6 had lipid binding activity.
- Taxon
- riceOryza sativat0
- Gene
- OsC6e0
- Outcome
- lipid binding activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“recombinant OsC6 has lipid binding activity”
t0“Rice”
Claim · model-written summary
OsC6 silencing caused defective orbicule and pollen exine development.
- Taxon
- riceOryza sativat0
- Treatment
- OsC6 was silencede0
- Gene
- OsC6e0
- Outcome
- development of orbicules (i.e. Ubisch bodies) and pollen exinee0
- Direction
- defectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plants in which OsC6 was silenced had defective development of orbicules (i.e. Ubisch bodies) and pollen exine”
t0“Rice”
Claim · model-written summary
OsC6 silencing reduced pollen fertility.
- Taxon
- riceOryza sativat0
- Treatment
- OsC6 was silencede0
- Gene
- OsC6e0
- Outcome
- pollen fertilitye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plants in which OsC6 was silenced had defective development of orbicules (i.e. Ubisch bodies) and pollen exine and had reduced pollen fertility”
t0“Rice”
Claim · model-written summary
TDR positively regulated OsC6 expression.
- Taxon
- riceOryza sativat0
- Gene
- Tapetum Degeneration Retardation (TDR)e0
- Outcome
- expression of OsC6e0
- Direction
- positively regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of OsC6 is positively regulated by a basic helix-loop-helix transcription factor, Tapetum Degeneration Retardation (TDR)”
t0“Rice”
Claim · model-written summary
Driving OsC6 with the TDR promoter produced extra granule-like structures compared with tdr mutant.
- Taxon
- riceOryza sativat0
- Treatment
- expression of OsC6 was driven by the TDR promotere0
- Gene
- OsC6e0
- Outcome
- granule-like structurese0
- Direction
- Extrae0
- Comparator
- tdr mutante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Extra granule-like structures were observed on the inner surface of the tdr tapetal layer when the expression of OsC6 was driven by the TDR promoter compared with the tdr mutant”
t0“Rice”
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