Abstract
BACKGROUND: Bacterial blight of rice, caused by Xanthomonas oryzae pv. oryzae (Xoo), is a devastating rice disease in Southeast Asia and West Africa. OsSWEET14, encoding a sugar transporter, is known to be a major susceptible gene of bacterial blight targeted by four different transcription activator-like (TAL) effectors from either Asian or African Xoo strains. However, the OsSWEET14 single knockout or promoter mutants in the Kitaake background are moderately resistant or even susceptible to African Xoo strains. Therefore, in this study, we knocked out OsSWEET14 in rice cv. Zhonghua 11 background for disease assessment. RESULTS: In this study, CRISPR/Cas9 was utilized to disrupt the function of OsSWEET14 by modifying its corresponding coding region in the genome of rice cv. Zhonghua 11 (CR-S14). In total, we obtained nine different OsSWEET14-mutant alleles. Besides conferring broad-spectrum resistance to Asian Xoo strains, tested mutant alleles also showed strong resistance to African Xoo strain AXO1947. Moreover, the expression of OsSWEET14 was detected in vascular tissues, including the stem, leaf sheath, leaf blade and root. The disruption of OsSWEET14 led to increased plant height without a reduction in yield. CONCLUSIONS: Disruption of OsSWEET14 in the Zhonghua 11 background is able to confer strong resistance to African Xoo strain AXO1947 and Asian Xoo strain PXO86. CR-S14 has normal reproductive growth and enhanced plant height under normal growth conditions. These results imply that CR-S14 may serve as a better tester line than sweet14 single-knockout mutant in the Kitaake background for the diagnostic kit for rice blight resistance. The genetic background and increased plant height need to be taken into consideration when utilizing OsSWEET14 for resistant rice breeding.
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Claims 8
Claim · model-written summary
The study obtained nine OsSWEET14 mutant alleles in Zhonghua 11 rice.
- Taxon
- rice
Oryza sativat0 - Gene
- OsSWEET14e0
- Outcome
- OsSWEET14-mutant allelese0
- Value
- ninee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In total, we obtained nine different OsSWEET14-mutant alleles”
t0“OsSWEET14 in rice”
Claim · model-written summary
Tested OsSWEET14 mutant alleles conferred broad resistance to Asian Xoo strains.
- Taxon
- riceOryza sativat0
- Treatment
- OsSWEET14t0
- Interactor
- Xooe0pathogen
- Outcome
- resistance to Asian Xoo strainse0
- Direction
- broad-spectrume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Besides conferring broad-spectrum resistance to Asian Xoo strains, tested mutant alleles also showed strong resistance to African Xoo strain AXO1947”
t0“OsSWEET14 in rice”
Claim · model-written summary
Tested OsSWEET14 mutant alleles showed strong resistance to African Xoo strain AXO1947.
- Taxon
- riceOryza sativat0
- Treatment
- OsSWEET14t0
- Interactor
- Xoo strain AXO1947e0pathogen
- Outcome
- resistance to African Xoo strain AXO1947e0
- Direction
- stronge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tested mutant alleles also showed strong resistance to African Xoo strain AXO1947”
t0“OsSWEET14 in rice”
Claim · model-written summary
OsSWEET14 expression was detected in stem, leaf sheath, leaf blade and root vascular tissues.
- Taxon
- riceOryza sativat0
- Organ / stage
- vascular tissues, including the stem, leaf sheath, leaf blade and roote0
- Gene
- OsSWEET14e0
- Outcome
- expression of OsSWEET14e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of OsSWEET14 was detected in vascular tissues, including the stem, leaf sheath, leaf blade and root”
t0“OsSWEET14 in rice”
Claim · model-written summary
OsSWEET14 disruption increased rice plant height.
- Taxon
- riceOryza sativat0
- Treatment
- disruption of OsSWEET14e0
- Outcome
- plant heighte0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The disruption of OsSWEET14 led to increased plant height without a reduction in yield”
t0“OsSWEET14 in rice”
Claim · model-written summary
OsSWEET14 disruption did not reduce rice yield.
- Taxon
- riceOryza sativat0
- Treatment
- disruption of OsSWEET14e0
- Outcome
- yielde0
- Direction
- without a reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The disruption of OsSWEET14 led to increased plant height without a reduction in yield”
t0“OsSWEET14 in rice”
Claim · model-written summary
CR-S14 had normal reproductive growth under normal growth conditions.
- Taxon
- riceOryza sativat0
- Material
- CR-S14e0
- Outcome
- reproductive growthe0
- Direction
- normale0
- Qualifier
- normal growth conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CR-S14 has normal reproductive growth and enhanced plant height under normal growth conditions”
t0“OsSWEET14 in rice”
Claim · model-written summary
Authors propose CR-S14 as a better tester line for rice blight resistance diagnosis.
- Taxon
- riceOryza sativat0
- Material
- CR-S14e0
- Outcome
- tester linee0
- Comparator
- sweet14 single-knockout mutant in the Kitaake backgrounde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CR-S14 may serve as a better tester line than sweet14 single-knockout mutant in the Kitaake background for the diagnostic kit for rice blight resistance”
t0“OsSWEET14 in rice”
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