Abstract
Rice (Oryza sativa L.) produces ent-copalyl diphosphate (ent-CDP) and syn-CDP as precursors for several classes of phytoalexins and the phytohormones, gibberellins (GAs). It has recently been shown that a loss-of-function mutation of OsCPS1, a gene encoding a putative ent-CDP synthase, results in a severely GA-deficient dwarf phenotype in rice. To clarify the biological functions of the ent- and syn-CDP synthases involved in the biosynthesis of phytoalexins and/or GAs, we isolated two cDNAs, OsCyc1 and OsCyc2, encoding putative diterpene cyclases from ultraviolet (UV)-irradiated rice leaves (cv. Nipponbare). The production of phytoalexins in rice leaves is known to be highly induced by UV treatment. Using a bacterial expression system, we demonstrated that OsCyc1 encodes syn-CDP synthase and that OsCyc2 and OsCPS1 encode ent-CDP synthase. The level of expression of the OsCyc1 and OsCyc2 transcripts in rice leaves increased drastically in response to UV treatment, whereas expression of the OsCPS1 transcript was not induced by UV light. These results suggest that OsCyc1, OsCyc2 and OsCPS1 are responsible for the biosynthesis of momilactones A and B and oryzalexin S, oryzalexins A-F and phytocassanes A-E, and GAs, respectively. Our results strongly suggest the presence of two ent-CDP synthase isoforms in rice, one that participates in the biosynthesis of GAs and a second that is involved in the biosynthesis of phytoalexins.
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Claims 8
Claim · model-written summary
OsCyc1 encodes syn-CDP synthase in rice, as demonstrated using a bacterial expression system.
- Taxon
- rice
Oryza sativat0 - Gene
- OsCyc1e0
- Outcome
- encodes syn-CDP synthasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“OsCyc1 encodes syn-CDP synthase”
Claim · model-written summary
OsCyc2 and OsCPS1 encode ent-CDP synthase in rice, as demonstrated using a bacterial expression system.
- Taxon
- riceOryza sativat0
- Gene
- OsCyc2 and OsCPS1e0
- Outcome
- encode ent-CDP synthasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“OsCyc2 and OsCPS1 encode ent-CDP synthase”
Claim · model-written summary
Two cDNAs, OsCyc1 and OsCyc2, encoding putative diterpene cyclases were isolated from UV-irradiated rice leaves of cv. Nipponbare.
- Taxon
- riceOryza sativae0
- Material
- Nipponbaree0
- Organ / stage
- rice leavese0
- Treatment
- ultraviolet (UV)-irradiatede0
- Gene
- OsCyc1 and OsCyc2e0
- Outcome
- encoding putative diterpene cyclasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated two cDNAs, OsCyc1 and OsCyc2, encoding putative diterpene cyclases from ultraviolet (UV)-irradiated rice leaves (cv. Nipponbare)”
Claim · model-written summary
Expression of the OsCyc1 and OsCyc2 transcripts in rice leaves increased drastically in response to UV treatment.
- Taxon
- riceOryza sativae0
- Organ / stage
- rice leavese0
- Treatment
- UV treatmente0
- Gene
- OsCyc1 and OsCyc2e0
- Outcome
- level of expression of the OsCyc1 and OsCyc2 transcriptse0
- Direction
- increased drasticallye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The level of expression of the OsCyc1 and OsCyc2 transcripts in rice leaves increased drastically in response to UV treatment”
Claim · model-written summary
Expression of the OsCPS1 transcript was not induced by UV light.
- Taxon
- riceOryza sativat0
- Treatment
- UV lighte0
- Gene
- OsCPS1e0
- Outcome
- expression of the OsCPS1 transcripte0
- Direction
- not inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“expression of the OsCPS1 transcript was not induced by UV light”
Claim · model-written summary
The authors suggest OsCyc1, OsCyc2 and OsCPS1 are responsible for the biosynthesis of momilactones and oryzalexin S, oryzalexins and phytocassanes, and GAs, respectively.
- Taxon
- riceOryza sativat0
- Gene
- OsCyc1, OsCyc2 and OsCPS1e0
- Outcome
- biosynthesis of momilactones A and B and oryzalexin S, oryzalexins A-F and phytocassanes A-E, and GAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“OsCyc1, OsCyc2 and OsCPS1 are responsible for the biosynthesis of momilactones A and B and oryzalexin S, oryzalexins A-F and phytocassanes A-E, and GAs”
Claim · model-written summary
The authors strongly suggest two ent-CDP synthase isoforms exist in rice, one in GA biosynthesis and a second in phytoalexin biosynthesis.
- Taxon
- riceOryza sativae0
- Outcome
- presence of two ent-CDP synthase isoformse0
- Qualifier
- one that participates in the biosynthesis of GAs and a second that is involved in the biosynthesis of phytoalexinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the presence of two ent-CDP synthase isoforms in rice, one that participates in the biosynthesis of GAs and a second that is involved in the biosynthesis of phytoalexins”
Claim · model-written summary
A loss-of-function mutation of OsCPS1 was previously shown to give a severely GA-deficient dwarf phenotype in rice.
- Taxon
- riceOryza sativae0
- Gene
- OsCPS1e0
- Outcome
- severely GA-deficient dwarf phenotypee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a loss-of-function mutation of OsCPS1, a gene encoding a putative ent-CDP synthase, results in a severely GA-deficient dwarf phenotype in rice”
The same questions in other papers
uv · molecular: expression · Oryza sativa (rice)Positive and negative results
2 papers in the same direction as this paper · 7 in the opposite direction
- positive
- positive
- positive
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See all 10 papers →light · molecular: expression · Oryza sativa (rice)Positive and negative results
11 papers in the same direction as this paper · 20 in the opposite direction
- positive
- positive
- positive
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See all 32 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.