Abstract
Rice blast, caused by the fungal pathogen Magnaporthe grisea, is one of the most serious diseases of rice. Here we describe the isolation and characterization of Pib, one of the rice blast resistance genes. The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
The rice blast resistance gene Pib was isolated by map-based cloning.
- Taxon
- rice
Oryza sativat0 - Gene
- Pibe0
- Outcome
- Pib gene was isolatede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Pib gene was isolated by a map-based cloning strategy”
t0“rice blast resistance”
Claim · model-written summary
Pib protein has an NBS and LRRs and belongs to the NBS-LRR resistance gene class.
- Taxon
- riceOryza sativat0
- Gene
- Pibe0
- Outcome
- member of the NBS-LRR class of plant disease resistance genese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes”
t0“rice blast resistance”
Claim · model-written summary
The Pib protein has duplicated kinase motifs in its N terminal NBS region.
- Taxon
- riceOryza sativat0
- Gene
- Pib proteine0
- Outcome
- duplication of the kinase 1a, 2 and 3a motifse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein”
t0“rice blast resistance”
Claim · model-written summary
Eight cysteine residues cluster in the middle of Pib LRRs, a feature not previously reported for other R genes.
- Taxon
- riceOryza sativat0
- Gene
- Pibt1
- Outcome
- eight cysteine residues are clustered in the middle of the LRRse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes”
t0“rice blast resistance”
t1“Pib gene”
Claim · model-written summary
Altered temperatures and darkness induced Pib expression.
- Taxon
- riceOryza sativat0
- Treatment
- altered temperatures and darknesse0
- Gene
- Pibe0
- Outcome
- Pib gene expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness”
t0“rice blast resistance”
The same questions in other papers
light · molecular: expression · Oryza sativa (rice)Positive and negative results
19 papers in the same direction as this paper · 12 in the opposite direction
- no effect
- negative
- no effect
28 papers more in this group
See all 32 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.