Abstract
A functional analysis of the V2 protein of two monopartite begomoviruses, Papaya leaf curl virus (PaLCuV) and Cotton leaf curl Kokhran virus (CLCuKoV), has been performed. Expression of the V2 gene from a Potato virus X (PVX) vector resulted in severe leaf curling followed by a hypersensitive response (HR) in Nicotiana benthamiana and N. tabacum, demonstrating that the V2 protein is a pathogenicity determinant and a target of host defence responses. Agroinfiltration of a PVX vector expressing the V2 protein resulted in cell death in the infiltrated area. Subsequently, a systemic HR developed that was associated with the long-distance spread of the virus and led to the death of the plant. V2 amino acid sequences encompassing a conserved putative protein kinase C (PKC) phosphorylation motif were shown to be essential for the elicitation of cell death. In co-inoculation experiments, the transient expression of the C2 protein of PaLCuV or Cotton leaf curl Multan virus under the control of the Cauliflower mosaic virus 35S promoter inhibited the HR induced by V2 in the agroinfiltrated area. These findings demonstrate that the V2 protein of monopartite begomoviruses is a pathogenicity determinant and induces an HR that can be suppressed by the C2 protein. The induction and suppression of HR have been demonstrated previously in bipartite begomoviruses and our results extend this to monopartite begomoviruses.
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Claims 7
Claim · model-written summary
PVX-vectored V2 caused severe leaf curling and hypersensitive response in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- Expression of the V2 gene from a Potato virus X (PVX) vectore0
- Interactor
- Potato virus X (PVX)Potato virus Xe0pathogen
- Gene
- V2 genee0
- Outcome
- severe leaf curling followed by a hypersensitive response (HR)e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of the V2 gene from a Potato virus X (PVX) vector resulted in severe leaf curling followed by a hypersensitive response (HR) in Nicotiana benthamiana and N. tabacum”
Claim · model-written summary
PVX-vectored V2 caused severe leaf curling and hypersensitive response in N. tabacum.
- Taxon
- N. tabacum
Nicotiana tabacume0 - Treatment
- Expression of the V2 gene from a Potato virus X (PVX) vectore0
- Interactor
- Potato virus X (PVX)Potato virus Xe0pathogen
- Gene
- V2 genee0
- Outcome
- severe leaf curling followed by a hypersensitive response (HR)e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of the V2 gene from a Potato virus X (PVX) vector resulted in severe leaf curling followed by a hypersensitive response (HR) in Nicotiana benthamiana and N. tabacum”
Claim · model-written summary
Agroinfiltration of a PVX vector expressing V2 caused local cell death.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Agroinfiltration of a PVX vector expressing the V2 proteine0
- Interactor
- PVXPotato virus Xe0pathogen
- Gene
- V2 proteine0
- Outcome
- cell death in the infiltrated areae0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Agroinfiltration of a PVX vector expressing the V2 protein resulted in cell death in the infiltrated area”
t0“Nicotiana benthamiana and N. tabacum”
Claim · model-written summary
Systemic hypersensitive response accompanied long-distance viral spread and killed the plant.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Outcome
- systemic HRe0
- Qualifier
- led to the death of the plante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a systemic HR developed that was associated with the long-distance spread of the virus and led to the death of the plant”
t0“Nicotiana benthamiana and N. tabacum”
Claim · model-written summary
A conserved putative PKC phosphorylation motif in V2 was essential for cell death elicitation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- V2e0
- Outcome
- elicitation of cell deathe0
- Qualifier
- conserved putative protein kinase C (PKC) phosphorylation motife0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“V2 amino acid sequences encompassing a conserved putative protein kinase C (PKC) phosphorylation motif were shown to be essential for the elicitation of cell death”
t0“Nicotiana benthamiana and N. tabacum”
Claim · model-written summary
PaLCuV C2 expression inhibited V2-induced hypersensitive response in the infiltrated area.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- transient expression of the C2 protein of PaLCuVe0
- Interactor
- PaLCuVe0pathogen
- Gene
- C2 protein of PaLCuVe0
- Outcome
- HR induced by V2e0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient expression of the C2 protein of PaLCuV or Cotton leaf curl Multan virus under the control of the Cauliflower mosaic virus 35S promoter inhibited the HR induced by V2 in the agroinfiltrated area”
t0“Nicotiana benthamiana and N. tabacum”
Claim · model-written summary
Cotton leaf curl Multan virus C2 expression inhibited V2-induced hypersensitive response in the infiltrated area.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- transient expression of the C2 protein of PaLCuV or Cotton leaf curl Multan viruse0
- Interactor
- Cotton leaf curl Multan virusCotton leaf curl Multan viruse0pathogen
- Gene
- C2 proteine0
- Outcome
- HR induced by V2e0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient expression of the C2 protein of PaLCuV or Cotton leaf curl Multan virus under the control of the Cauliflower mosaic virus 35S promoter inhibited the HR induced by V2 in the agroinfiltrated area”
t0“Nicotiana benthamiana and N. tabacum”
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See all 53 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.