Abstract
We have obtained transgenic tobaccos expressing the coat protein (CP) (CP+plants) or coat protein anti‐sense RNA [CP( —) plants] of the Russian isolate of potato virus X (PVXS). When these plants were challenged with seven different PVX isolates, two CP+ lines showed delay in virus propagation, whereas CP(—) lines were not protected at inoculum concentrations of 10 μg/ml. The level of protection in CP(+) lines was different for different PVX isolates. Sequence comparisons revealed that similarity between CPs of different PVX isolates to PVXS CP do not correlate with the protection achieved against these isolates in tobaccos expressing PVXS CP. We analyzed the replication of PVX isolates S, S6111 and P551 in protoplasts derived from PVX CP+ transgenic tobaccos. In contrast to studies with intact plants, we were not able to detect any differences in propagation of these isolates in individual CP+ tobacco cells. With 1 μg PVX inoculum, in CP+ protoplasts the replication of all three isolates was inhibited, whereas at higher inoculum concentrations no difference existed between CP+ and control cells. These data indicate that two mechanisms could be involved in PVX CP‐mediated virus protection: at low virus concentrations PVX replication is inhibited, and at higher virus concentrations PVX spread is perturbed in transgenic plants.
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Claims 8
Claim · model-written summary
Tobacco lines expressing PVX coat protein delayed propagation of seven different PVX isolates.
- Taxon
- tobacco
Nicotiana tabacumt0 - Material
- CP+ linese0
- Treatment
- coat protein expressingt0
- Interactor
- PVXPotato virus Xe0pathogen
- Outcome
- virus propagatione0
- Direction
- delaye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“PVX coat protein expressing tobacco plants”
e0“When these plants were challenged with seven different PVX isolates, two CP+ lines showed delay in virus propagation”
Claim · model-written summary
The level of protection in CP(+) tobacco lines differed among PVX isolates.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- CP(+) linese1
- Interactor
- PVXPotato virus Xe1pathogen
- Outcome
- level of protectione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“PVX coat protein expressing tobacco plants”
e1“The level of protection in CP(+) lines was different for different PVX isolates”
Claim · model-written summary
Similarity of coat protein sequences of PVX isolates to PVXS CP does not correlate with the protection achieved against these isolates in tobacco.
- Taxon
- tobaccoNicotiana tabacumt0
- Interactor
- PVXPotato virus Xe2pathogen
- Outcome
- protection achieved against these isolatese2
- Direction
- do not correlatee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“PVX coat protein expressing tobacco plants”
e2“similarity between CPs of different PVX isolates to PVXS CP do not correlate with the protection achieved against these isolates”
Claim · model-written summary
No differences in propagation of PVX isolates were detected in individual CP+ tobacco cells.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- individual CP+ tobacco cellse3
- Outcome
- propagation of these isolatese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“PVX coat protein expressing tobacco plants”
e3“we were not able to detect any differences in propagation of these isolates in individual CP+ tobacco cells”
Claim · model-written summary
At 1 μg PVX inoculum, replication of all three PVX isolates was inhibited in CP+ tobacco protoplasts.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- CP+ protoplastse4
- Treatment
- 1 μg PVX inoculume4
- Interactor
- PVXPotato virus Xe4pathogen
- Outcome
- replication of all three isolatese4
- Direction
- inhibitede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“PVX coat protein expressing tobacco plants”
e4“With 1 μg PVX inoculum, in CP+ protoplasts the replication of all three isolates was inhibited”
Claim · model-written summary
At higher PVX inoculum concentrations, no difference in replication existed between CP+ and control tobacco cells.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- higher inoculum concentrationse5
- Interactor
- PVXPotato virus Xt0pathogen
- Outcome
- differencee5
- Direction
- no differencee5
- Comparator
- control cellse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“PVX coat protein expressing tobacco plants”
e5“at higher inoculum concentrations no difference existed between CP+ and control cells”
Claim · model-written summary
PVX spread is perturbed at higher virus concentrations in transgenic tobacco plants, a mechanism proposed by the authors.
- Taxon
- tobaccoNicotiana tabacumt0
- Interactor
- PVXPotato virus Xe6pathogen
- Outcome
- PVX spreade6
- Qualifier
- at higher virus concentrationse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“PVX coat protein expressing tobacco plants”
e6“at higher virus concentrations PVX spread is perturbed in transgenic plants”
Claim · model-written summary
Coat protein anti-sense tobacco lines were not protected against PVX at inoculum concentrations of 10 μg/ml.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- inoculum concentrations of 10 μg/mle7
- Outcome
- protectede7
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“PVX coat protein expressing tobacco plants”
e7“were not protected at inoculum concentrations of 10 μg/ml”
The same questions in other papers
Solanum tuberosum · Potato virus X · Nicotiana tabacum (tobacco)Positive and negative results
2 papers in the same direction as this paper · 10 in the opposite direction
- negative
- no effect
- negative
9 papers more in this group
See all 13 papers →inoculation · Potato virus X · Nicotiana tabacum (tobacco)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.