Abstract
In this paper we report on our study of the changes in biomass, lipid composition, and fermentation end products, as well as in the ATP level and synthesis rate in cultivated potato (Solanum tuberosum) cells submitted to anoxia stress. During the first phase of about 12 h, cells coped with the reduced energy supply brought about by fermentation and their membrane lipids remained intact. The second phase (12-24 h), during which the energy supply dropped down to 1% to 2% of its maximal theoretical normoxic value, was characterized by an extensive hydrolysis of membrane lipids to free fatty acids. This autolytic process was ascribed to the activation of a lipolytic acyl hydrolase. Cells were also treated under normoxia with inhibitors known to interfere with energy metabolism. Carbonyl-cyanide-4-trifluoromethoxyphenylhydrazone did not induce lipid hydrolysis, which was also the case when sodium azide or salicylhydroxamic acid were fed separately. However, the simultaneous use of sodium azide plus salicylhydroxamic acid or 2-deoxy-D-glucose plus iodoacetate with normoxic cells promoted a lipid hydrolysis pattern similar to that seen in anoxic cells. Therefore, a threshold exists in the rate of ATP synthesis (approximately 10 &mgr;mol g-1 fresh weight h-1), below which the integrity of the membranes in anoxic potato cells cannot be preserved.
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Claims 10
Claim · model-written summary
Potato cell membrane lipids remained intact during the first about 12 hours of anoxia.
- Taxon
- potato
Solanum tuberosume1 - Treatment
- anoxia stresse1
- Outcome
- membrane lipids remained intacte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During the first phase of about 12 h, cells coped with the reduced energy supply brought about by fermentation and their membrane lipids remained intact.”
e1“cultivated potato (Solanum tuberosum) cells submitted to anoxia stress”
Claim · model-written summary
Energy supply in anoxic potato cells dropped to 1% to 2% of maximal theoretical normoxic value in hours 12–24.
- Taxon
- potatoSolanum tuberosume1
- Outcome
- energy supplye0
- Direction
- dropped downe0
- Value
- 1% to 2%e0
- Comparator
- maximal theoretical normoxic valuee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The second phase (12-24 h), during which the energy supply dropped down to 1% to 2% of its maximal theoretical normoxic value”
e1“cultivated potato (Solanum tuberosum) cells submitted to anoxia stress”
Claim · model-written summary
Potato membrane lipids underwent extensive hydrolysis in hours 12–24 of anoxia.
- Taxon
- potatoSolanum tuberosume1
- Outcome
- hydrolysis of membrane lipids to free fatty acidse0
- Qualifier
- 12-24 he0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The second phase (12-24 h), during which the energy supply dropped down to 1% to 2% of its maximal theoretical normoxic value, was characterized by an extensive hydrolysis of membrane lipids to free fatty acids.”
e1“cultivated potato (Solanum tuberosum) cells submitted to anoxia stress”
Claim · model-written summary
Authors attributed lipid hydrolysis to activation of a lipolytic acyl hydrolase.
- Taxon
- potatoSolanum tuberosume2
- Gene
- lipolytic acyl hydrolasee0
- Outcome
- hydrolysis of membrane lipids to free fatty acidse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This autolytic process was ascribed to the activation of a lipolytic acyl hydrolase.”
e1“hydrolysis of membrane lipids to free fatty acids”
e2“cultivated potato (Solanum tuberosum) cells submitted to anoxia stress”
Claim · model-written summary
Carbonyl-cyanide-4-trifluoromethoxyphenylhydrazone did not induce potato lipid hydrolysis under normoxia.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- Carbonyl-cyanide-4-trifluoromethoxyphenylhydrazonee0
- Outcome
- lipid hydrolysise0
- Direction
- did not inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Carbonyl-cyanide-4-trifluoromethoxyphenylhydrazone did not induce lipid hydrolysis”
e1“cultivated potato (Solanum tuberosum) cells”
Claim · model-written summary
Sodium azide alone did not induce potato lipid hydrolysis.
- Taxon
- potatoSolanum tuberosume2
- Treatment
- sodium azidee0
- Outcome
- lipid hydrolysise1
- Direction
- did not inducee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which was also the case when sodium azide or salicylhydroxamic acid were fed separately”
e1“did not induce lipid hydrolysis”
e2“cultivated potato (Solanum tuberosum) cells”
Claim · model-written summary
Salicylhydroxamic acid alone did not induce potato lipid hydrolysis.
- Taxon
- potatoSolanum tuberosume2
- Treatment
- salicylhydroxamic acide0
- Outcome
- lipid hydrolysise1
- Direction
- did not inducee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which was also the case when sodium azide or salicylhydroxamic acid were fed separately”
e1“did not induce lipid hydrolysis”
e2“cultivated potato (Solanum tuberosum) cells”
Claim · model-written summary
Sodium azide plus salicylhydroxamic acid produced an anoxia-like lipid hydrolysis pattern under normoxia.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- sodium azide plus salicylhydroxamic acide0
- Outcome
- lipid hydrolysis patterne0
- Direction
- promotede0
- Comparator
- anoxic cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the simultaneous use of sodium azide plus salicylhydroxamic acid or 2-deoxy-D-glucose plus iodoacetate with normoxic cells promoted a lipid hydrolysis pattern similar to that seen in anoxic cells”
e1“cultivated potato (Solanum tuberosum) cells”
Claim · model-written summary
2-deoxy-D-glucose plus iodoacetate produced an anoxia-like lipid hydrolysis pattern under normoxia.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- 2-deoxy-D-glucose plus iodoacetatee0
- Outcome
- lipid hydrolysis patterne0
- Direction
- promotede0
- Comparator
- anoxic cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the simultaneous use of sodium azide plus salicylhydroxamic acid or 2-deoxy-D-glucose plus iodoacetate with normoxic cells promoted a lipid hydrolysis pattern similar to that seen in anoxic cells”
e1“cultivated potato (Solanum tuberosum) cells”
Claim · model-written summary
Authors infer membrane integrity fails below an ATP synthesis threshold of approximately 10 micromoles per gram fresh weight per hour.
- Taxon
- potatoSolanum tuberosume0
- Outcome
- integrity of the membranese0
- Value
- approximately 10 &mgr;mol g-1 fresh weight h-1e0
- Qualifier
- rate of ATP synthesise0
Not reportedGene
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a threshold exists in the rate of ATP synthesis (approximately 10 &mgr;mol g-1 fresh weight h-1), below which the integrity of the membranes in anoxic potato cells cannot be preserved”
The same questions in other papers
sodium azide · molecular: metabolites · Solanum tuberosum (potato)Positive and negative results
1 in the opposite direction
- positive
salicylhydroxamic acid · molecular: metabolites · Solanum tuberosum (potato)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.