Abstract
본 연구에서는 고추의 소포자 배양 시 전처리 후 배지의 교환 여부, 배양 후 새 배지의 첨가 및 2층배지 사용시 하층고체배지의 양이 배의 생산에 미치는 영향을 조사하였다. 고온 전처리 후 배양배지를 첨가하여 배양하는 것보다 사용한 전처리 배지를 새 배양 배지로 교환하여 배양 하는 것이 배의 생산에 효과적 이었으며, 고온전처리 기간은 1일이나 2일에 비해 3일이 효과적 이었다. 배양 후 새 배지의 첨가는 약전처리 시에는 효과가 없었으나 소포자 전처리 시에는 배의 유기와 발달 모두 크게 향상되었다. 새 배지의 첨가 시기는 배양 4일 후가, 첨가 횟수는 1회가 배의 생산에 가장 효과적이었다. 한편 2층배지 사용 시 첨가하는 새 배지의 양은 1.5 ml이 효과적이었으며 이보다 많은 양을 첨가하는 경우 배의 발생과 발달 모두 저하되었다. 액체배지 사용 시에 비해 2층배지 사용 시 배의 발달이 좋았다. 또 2층배지 사용 시 하층고체배지의 양이 3 ml 일 때 보다는 5 ml이나 7 ml일 때 배의 발생은 감소하였으나 배의 질이 향상되었다. 이와 같은 결과들은 고추에서 다수의 정상자엽배를 생산 할 수 있는 소포자 배양시스템을 확립하는데 중요한 기초자료가 될 것이다. The effect of the addition of the fresh medium, volume of under solid medium in double layer culture as well as the medium change after pretreating microspores on the production of embryos in microspore culture of hot pepper (Capsicum annuum L.) has been studied. When cultured after heat pre-treatment, changing pretreatment media with fresh culture media proved to be more effective for embryo production rather than supplementing additional culture media. Heat-pretreating for 3 days turned out more effective for embryo production than pretreating for 1 or 2 days. In the case of anther pretreatment, the addition of fresh medium after culture was not effective for embryo production. In pretreating microspores, however, supplementing additional fresh culture media greatly improved embryo yield and quality. The best time point of media addition was 4 days after culture commenced, and the most effective number of times of media addition was one time addition. Moreover, the effective volume of added medium in double layer culture for embryo production was 1.5 ml. The addition of media more than 1.5 ml reduced both embryo yield and quality. Double layer medium was more effective for embryo development than liquid medium. When the volume of under solid medium increased ranging from 3 ml to 7 ml, more cotyledonary embryos were produced in either 5 ml or 7 ml compared to 3 ml, even though the total number of embryos were highest in 3 ml. These results can be used as an important data for establishing an efficient microspore culture system for producing high frequency of normal embryos in hot pepper.
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Claims 11
Claim · model-written summary
Replacing pretreatment medium after heat treatment improved embryo production.
- Taxon
- hot pepper (Capsicum annuum L.)
Capsicum annuumt0 - Treatment
- changing pretreatment media with fresh culture mediae0
- Outcome
- embryo productione0
- Direction
- more effectivee0
- Comparator
- supplementing additional culture mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“changing pretreatment media with fresh culture media proved to be more effective for embryo production rather than supplementing additional culture media”
t0“hot pepper (Capsicum annuum L.)”
Claim · model-written summary
Three days of heat pretreatment improved embryo production over one or two days.
- Taxon
- hot pepper (Capsicum annuum L.)Capsicum annuumt0
- Treatment
- Heat-pretreating for 3 dayse0
- Outcome
- embryo productione0
- Direction
- more effectivee0
- Comparator
- pretreating for 1 or 2 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Heat-pretreating for 3 days turned out more effective for embryo production than pretreating for 1 or 2 days.”
t0“hot pepper (Capsicum annuum L.)”
Claim · model-written summary
Fresh medium addition after anther pretreatment did not improve embryo production.
- Taxon
- hot pepper (Capsicum annuum L.)Capsicum annuumt0
- Treatment
- addition of fresh medium after culturee0
- Outcome
- embryo productione0
- Direction
- not effectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the case of anther pretreatment, the addition of fresh medium after culture was not effective for embryo production.”
t0“hot pepper (Capsicum annuum L.)”
Claim · model-written summary
Fresh medium after microspore pretreatment improved embryo yield and quality.
- Taxon
- hot pepper (Capsicum annuum L.)Capsicum annuumt0
- Treatment
- supplementing additional fresh culture mediae0
- Outcome
- embryo yield and qualitye0
- Direction
- greatly improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In pretreating microspores, however, supplementing additional fresh culture media greatly improved embryo yield and quality.”
t0“hot pepper (Capsicum annuum L.)”
Claim · model-written summary
Adding medium four days after culture was most effective.
- Taxon
- hot pepper (Capsicum annuum L.)Capsicum annuumt0
- Treatment
- media additione0
- Outcome
- best time pointe0
- Direction
- beste0
- Value
- 4 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best time point of media addition was 4 days after culture commenced”
t0“hot pepper (Capsicum annuum L.)”
Claim · model-written summary
A single medium addition was most effective.
- Taxon
- hot pepper (Capsicum annuum L.)Capsicum annuumt0
- Treatment
- one time additione0
- Outcome
- most effective number of times of media additione0
- Direction
- most effectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the most effective number of times of media addition was one time addition”
t0“hot pepper (Capsicum annuum L.)”
Claim · model-written summary
Adding 1.5 ml medium was effective for embryo production.
- Taxon
- hot pepper (Capsicum annuum L.)Capsicum annuumt0
- Treatment
- added medium in double layer culturee0
- Outcome
- embryo productione0
- Direction
- effectivee0
- Value
- 1.5 mle0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the effective volume of added medium in double layer culture for embryo production was 1.5 ml”
t0“hot pepper (Capsicum annuum L.)”
Claim · model-written summary
More than 1.5 ml added medium reduced embryo yield and quality.
- Taxon
- hot pepper (Capsicum annuum L.)Capsicum annuumt0
- Treatment
- addition of media more than 1.5 mle0
- Outcome
- embryo yield and qualitye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of media more than 1.5 ml reduced both embryo yield and quality.”
t0“hot pepper (Capsicum annuum L.)”
Claim · model-written summary
Double layer medium improved embryo development compared with liquid medium.
- Taxon
- hot pepper (Capsicum annuum L.)Capsicum annuumt0
- Treatment
- Double layer mediume0
- Outcome
- embryo developmente0
- Direction
- more effectivee0
- Comparator
- liquid mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Double layer medium was more effective for embryo development than liquid medium.”
t0“hot pepper (Capsicum annuum L.)”
Claim · model-written summary
Five or seven ml of solid medium produced more cotyledonary embryos than three ml.
- Taxon
- hot pepper (Capsicum annuum L.)Capsicum annuumt0
- Treatment
- 5 ml or 7 mle0
- Outcome
- cotyledonary embryose0
- Direction
- moree0
- Comparator
- 3 mle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“more cotyledonary embryos were produced in either 5 ml or 7 ml compared to 3 ml”
t0“hot pepper (Capsicum annuum L.)”
Claim · model-written summary
Three ml of solid medium produced the highest total number of embryos.
- Taxon
- hot pepper (Capsicum annuum L.)Capsicum annuumt0
- Treatment
- 3 mle0
- Outcome
- total number of embryose0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the total number of embryos were highest in 3 ml”
t0“hot pepper (Capsicum annuum L.)”
The same questions in other papers
in vitro · quality: general · Capsicum annuum (sweet pepper)Positive and negative results
1 in the opposite direction
- no effect
in vitro · yield · Capsicum annuum (sweet pepper)Positive and negative results
2 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.