Abstract
The influences of pretreatment medium, the addition of fresh medium, and the size of culture plate on the production of embryos were investigated in isolated microspore culture of hot pepper (Capsicum annuum L.). Among the media used for heat shock pretreatment ($32{\pm}1{^{\circ}C}$), high frequency embryo production was obtained when the sucrosestarvation medium A was used. On the other hand, neither 0.37 M mannitol solution nor NLNS medium supplemented with sucrose was not efficient for embryo production. The addition of culture medium to pretreatment media considerably decreased the embryo production even though embryo development proceeded further. The embryo production was not improved by the addition of fresh medium after 2 or 3 weeks from starting culture. Increase in the size of the culture plate from $3.5{\times}1.0$ cm to $6.0{\times}1.5$ cm improved embryo quality. These results will provide valuable information for developing an efficient microspore culture system of hot pepper for high frequency embryo production.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Sucrose-starvation medium A gave high embryo production under heat shock.
- Taxon
- hot pepper (Capsicum annuum L.)
Capsicum annuumt0 - Treatment
- sucrosestarvation medium Ae0
- Outcome
- embryo productione0
- Direction
- high frequencye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high frequency embryo production was obtained when the sucrosestarvation medium A was used”
t0“hot pepper (Capsicum annuum L.)”
Claim · model-written summary
Mannitol solution was inefficient for embryo production.
- Taxon
- hot pepper (Capsicum annuum L.)Capsicum annuumt0
- Treatment
- 0.37 M mannitol solutione0
- Outcome
- embryo productione0
- Direction
- not efficiente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“neither 0.37 M mannitol solution nor NLNS medium supplemented with sucrose was not efficient for embryo production”
t0“hot pepper (Capsicum annuum L.)”
Claim · model-written summary
Sucrose-supplemented NLNS medium was inefficient for embryo production.
- Taxon
- hot pepper (Capsicum annuum L.)Capsicum annuumt0
- Treatment
- NLNS medium supplemented with sucrosee0
- Outcome
- embryo productione0
- Direction
- not efficiente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“neither 0.37 M mannitol solution nor NLNS medium supplemented with sucrose was not efficient for embryo production”
t0“hot pepper (Capsicum annuum L.)”
Claim · model-written summary
Adding culture medium to pretreatment media decreased embryo production.
- Taxon
- hot pepper (Capsicum annuum L.)Capsicum annuumt0
- Treatment
- addition of culture medium to pretreatment mediae0
- Outcome
- embryo productione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of culture medium to pretreatment media considerably decreased the embryo production even though embryo development proceeded further”
t0“hot pepper (Capsicum annuum L.)”
Claim · model-written summary
Adding culture medium to pretreatment media advanced embryo development.
- Taxon
- hot pepper (Capsicum annuum L.)Capsicum annuumt0
- Treatment
- addition of culture medium to pretreatment mediae0
- Outcome
- embryo developmente0
- Direction
- proceeded furthere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of culture medium to pretreatment media considerably decreased the embryo production even though embryo development proceeded further”
t0“hot pepper (Capsicum annuum L.)”
Claim · model-written summary
Fresh medium added after two or three weeks did not improve embryo production.
- Taxon
- hot pepper (Capsicum annuum L.)Capsicum annuumt0
- Treatment
- addition of fresh medium after 2 or 3 weekse0
- Outcome
- embryo productione0
- Direction
- not improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The embryo production was not improved by the addition of fresh medium after 2 or 3 weeks from starting culture”
t0“hot pepper (Capsicum annuum L.)”
Claim · model-written summary
Larger culture plates improved embryo quality.
- Taxon
- hot pepper (Capsicum annuum L.)Capsicum annuumt0
- Treatment
- $6.0{\times}1.5$ cme0
- Outcome
- embryo qualitye0
- Direction
- improvede0
- Comparator
- $3.5{\times}1.0$ cme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increase in the size of the culture plate from $3.5{\times}1.0$ cm to $6.0{\times}1.5$ cm improved embryo quality”
t0“hot pepper (Capsicum annuum L.)”
The same questions in other papers
in vitro · yield · Capsicum annuum (sweet pepper)Positive and negative results
3 papers in the same direction as this paper · 3 in the opposite direction
- negative
- no effect
- no effect
3 papers more in this group
See all 7 papers →in vitro · growth · Capsicum annuum (sweet pepper)Results agree
18 papers in the same direction as this paper
- positive
- positive
- positive
15 papers more in this group
See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.