Abstract
Transgenic tomato (Solanum lycopersicum) plants, expressing a fragment of the mitochondrial citrate synthase gene in the antisense orientation and exhibiting mild reductions in the total cellular activity of this enzyme, displayed essentially no visible phenotypic alteration from the wild type. A more detailed physiological characterization, however, revealed that although these plants were characterized by relatively few changes in photosynthetic parameters they displayed a decreased relative flux through the tricarboxylic acid cycle and an increased rate of respiration. Furthermore, biochemical analyses revealed that the transformants exhibited considerably altered metabolism, being characterized by slight decreases in the levels of organic acids of the tricarboxylic acid cycle, photosynthetic pigments, and in a single line in protein content but increases in the levels of nitrate, several amino acids, and starch. We additionally determined the maximal catalytic activities of a wide range of enzymes of primary metabolism, performed targeted quantitative PCR analysis on all three isoforms of citrate synthase, and conducted a broader transcript profiling using the TOM1 microarray. Results from these studies confirmed that if the lines were somewhat impaired in nitrate assimilation, they were not severely affected by this, suggesting the presence of strategies by which metabolism is reprogrammed to compensate for this deficiency. The results are discussed in the context of carbon-nitrogen interaction and interorganellar coordination of metabolism.
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Claims 9
Claim · model-written summary
Antisense tomato plants showed no visible phenotypic alteration from wild type.
- Taxon
- Solanum lycopersicum
Solanum lycopersicume0 - Treatment
- expressing a fragment of the mitochondrial citrate synthase gene in the antisense orientatione0
- Outcome
- visible phenotypic alteratione0
- Direction
- no visiblee0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic tomato (Solanum lycopersicum) plants, expressing a fragment of the mitochondrial citrate synthase gene in the antisense orientation and exhibiting mild reductions in the total cellular activity of this enzyme, displayed essentially no visible phenotypic alteration from the wild type.”
Claim · model-written summary
Antisense tomato plants had few changes in photosynthetic parameters.
- Taxon
- Solanum lycopersicumSolanum lycopersicume1
- Treatment
- antisensee1
- Outcome
- photosynthetic parameterse0
- Direction
- few changese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A more detailed physiological characterization, however, revealed that although these plants were characterized by relatively few changes in photosynthetic parameters they displayed a decreased relative flux through the tricarboxylic acid cycle and an increased rate of respiration.”
t0“Mitochondrial Citrate Synthase Activity”
e1“Transgenic tomato (Solanum lycopersicum) plants, expressing a fragment of the mitochondrial citrate synthase gene in the antisense orientation and exhibiting mild reductions in the total cellular activity of this enzyme, displayed essentially no visible phenotypic alteration from the wild type.”
Claim · model-written summary
Antisense plants had reduced relative tricarboxylic-acid-cycle flux.
- Taxon
- Solanum lycopersicumSolanum lycopersicume1
- Treatment
- antisensee1
- Outcome
- relative flux through the tricarboxylic acid cyclee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A more detailed physiological characterization, however, revealed that although these plants were characterized by relatively few changes in photosynthetic parameters they displayed a decreased relative flux through the tricarboxylic acid cycle and an increased rate of respiration.”
t0“Mitochondrial Citrate Synthase Activity”
e1“Transgenic tomato (Solanum lycopersicum) plants, expressing a fragment of the mitochondrial citrate synthase gene in the antisense orientation and exhibiting mild reductions in the total cellular activity of this enzyme, displayed essentially no visible phenotypic alteration from the wild type.”
Claim · model-written summary
Antisense plants had increased respiration.
- Taxon
- Solanum lycopersicumSolanum lycopersicume1
- Treatment
- antisensee1
- Outcome
- rate of respiratione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A more detailed physiological characterization, however, revealed that although these plants were characterized by relatively few changes in photosynthetic parameters they displayed a decreased relative flux through the tricarboxylic acid cycle and an increased rate of respiration.”
t0“Mitochondrial Citrate Synthase Activity”
e1“Transgenic tomato (Solanum lycopersicum) plants, expressing a fragment of the mitochondrial citrate synthase gene in the antisense orientation and exhibiting mild reductions in the total cellular activity of this enzyme, displayed essentially no visible phenotypic alteration from the wild type.”
Claim · model-written summary
Transformants had lower tricarboxylic acid organic acids and photosynthetic pigments.
- Taxon
- Solanum lycopersicumSolanum lycopersicume1
- Treatment
- transformantse0
- Outcome
- levels of organic acids of the tricarboxylic acid cycle, photosynthetic pigmentse0
- Direction
- decreasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, biochemical analyses revealed that the transformants exhibited considerably altered metabolism, being characterized by slight decreases in the levels of organic acids of the tricarboxylic acid cycle, photosynthetic pigments, and in a single line in protein content but increases in the levels of nitrate, several amino acids, and starch.”
t0“Mitochondrial Citrate Synthase Activity”
e1“Transgenic tomato (Solanum lycopersicum) plants, expressing a fragment of the mitochondrial citrate synthase gene in the antisense orientation and exhibiting mild reductions in the total cellular activity of this enzyme, displayed essentially no visible phenotypic alteration from the wild type.”
Claim · model-written summary
One transformant line had reduced protein content.
- Taxon
- Solanum lycopersicumSolanum lycopersicume1
- Treatment
- transformantse0
- Outcome
- protein contente0
- Direction
- decreasese0
- Qualifier
- in a single linee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, biochemical analyses revealed that the transformants exhibited considerably altered metabolism, being characterized by slight decreases in the levels of organic acids of the tricarboxylic acid cycle, photosynthetic pigments, and in a single line in protein content but increases in the levels of nitrate, several amino acids, and starch.”
t0“Mitochondrial Citrate Synthase Activity”
e1“Transgenic tomato (Solanum lycopersicum) plants, expressing a fragment of the mitochondrial citrate synthase gene in the antisense orientation and exhibiting mild reductions in the total cellular activity of this enzyme, displayed essentially no visible phenotypic alteration from the wild type.”
Claim · model-written summary
Transformants had higher nitrate, amino acid and starch levels.
- Taxon
- Solanum lycopersicumSolanum lycopersicume1
- Treatment
- transformantse0
- Outcome
- levels of nitrate, several amino acids, and starche0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, biochemical analyses revealed that the transformants exhibited considerably altered metabolism, being characterized by slight decreases in the levels of organic acids of the tricarboxylic acid cycle, photosynthetic pigments, and in a single line in protein content but increases in the levels of nitrate, several amino acids, and starch.”
t0“Mitochondrial Citrate Synthase Activity”
e1“Transgenic tomato (Solanum lycopersicum) plants, expressing a fragment of the mitochondrial citrate synthase gene in the antisense orientation and exhibiting mild reductions in the total cellular activity of this enzyme, displayed essentially no visible phenotypic alteration from the wild type.”
Claim · model-written summary
The lines were somewhat impaired in nitrate assimilation, but not severely affected.
- Taxon
- Solanum lycopersicumSolanum lycopersicume1
- Outcome
- impaired in nitrate assimilatione0
- Qualifier
- not severely affectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Results from these studies confirmed that if the lines were somewhat impaired in nitrate assimilation, they were not severely affected by this, suggesting the presence of strategies by which metabolism is reprogrammed to compensate for this deficiency.”
t0“Mitochondrial Citrate Synthase Activity”
e1“Transgenic tomato (Solanum lycopersicum) plants, expressing a fragment of the mitochondrial citrate synthase gene in the antisense orientation and exhibiting mild reductions in the total cellular activity of this enzyme, displayed essentially no visible phenotypic alteration from the wild type.”
Claim · model-written summary
The authors suggest compensatory metabolic reprogramming in the lines.
- Taxon
- Solanum lycopersicumSolanum lycopersicume1
- Outcome
- metabolism is reprogrammed to compensate for this deficiencye0
- Qualifier
- strategiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Results from these studies confirmed that if the lines were somewhat impaired in nitrate assimilation, they were not severely affected by this, suggesting the presence of strategies by which metabolism is reprogrammed to compensate for this deficiency.”
t0“Mitochondrial Citrate Synthase Activity”
e1“Transgenic tomato (Solanum lycopersicum) plants, expressing a fragment of the mitochondrial citrate synthase gene in the antisense orientation and exhibiting mild reductions in the total cellular activity of this enzyme, displayed essentially no visible phenotypic alteration from the wild type.”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.