Abstract
Improving plant water-use efficiency (WUE) is important to plant survival and crop yield in the context of water limitation. In this study, SlTLFP8 (Tubby-like F-box protein 8) was identified as an osmotic-induced gene in tomato. Transgenic tomato with up-regulated expression of SlTLFP8 showed enhanced water-deficient resistance, whereas knockout mutants generated by CRISPR/Cas9 were more sensitive to water deficit. SlTLFP8 overexpression significantly enhanced WUE by suppressing transpiration under both water-sufficient and water-deficient conditions. Further study showed that overexpressing SlTLFP8 significantly increased leaf epidermal cell size and thereby decreased stomatal density 10-20%, conversely SlTLFP8 knockout resulted in decreased cell size and thereby increased stomatal density 20-50%. SlTLFP8 overexpression and knockout modulated ploidy levels in leaf cells. Changes in expression of cell cycle related genes also indicated that SlTLFP8 affected cell size and stomatal density through endocycle transition. Despite changes in stomata density and transpiration, altering the expression of SlTLFP8 did not change photosynthesis. Additionally, biomass was not altered and there was little difference in fruit yield for transgenic and wild type lines under water-sufficient and water-deficient conditions. Our results demonstrate the effect of SlTLFP8 on endoreduplication and the potential of SlTLFP8 for improvement of WUE. BRIEF SUMMERY: This work found a new mechanism of TLP (Tubby like protein) response to water-deficient stress. SlTLFP8, a member of TLP family, regulates water-deficient resistance by modulating water loss via affecting stomatal density. Expression of SlTLFP8 was induced by osmotic stress. Transgenic tomato lines with SlTLFP8 overexpression or SlTLFP8 knockout showed significantly differences in water-use efficiency (WUE) and water-deficient resistance. The difference of leaf water loss caused by transpiration is the main explanation of the difference in WUE and water-deficient resistance. Additionally, overexpressing SlTLFP8 significantly decreased stomatal density, while SlTLFP8 knockout resulted in increased stomatal density, and SlTLFP8 affected stomatal density through endoreduplication and altered epidermal cell size. Despite changes in stomata density, altering the expression of SlTLFP8 did not result in distinct changes in photosynthesis, biomass and yield of tomato.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 14
Claim · model-written summary
SlTLFP8 was identified as an osmotic-induced gene in tomato.
- Taxon
- tomato
Solanum lycopersicume0 - Gene
- SlTLFP8e0
- Outcome
- osmotic-induced genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlTLFP8 (Tubby-like F-box protein 8) was identified as an osmotic-induced gene in tomato.”
Claim · model-written summary
SlTLFP8 up-regulation enhanced tomato resistance to water deficiency.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- up-regulated expression of SlTLFP8e0
- Gene
- SlTLFP8e0
- Outcome
- water-deficient resistancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic tomato with up-regulated expression of SlTLFP8 showed enhanced water-deficient resistance, whereas knockout mutants generated by CRISPR/Cas9 were more sensitive to water deficit.”
Claim · model-written summary
SlTLFP8 knockout mutants were more sensitive to water deficit.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- knockout mutants generated by CRISPR/Cas9e0
- Gene
- SlTLFP8e0
- Outcome
- sensitive to water deficite0
- Direction
- more sensitivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic tomato with up-regulated expression of SlTLFP8 showed enhanced water-deficient resistance, whereas knockout mutants generated by CRISPR/Cas9 were more sensitive to water deficit.”
Claim · model-written summary
SlTLFP8 overexpression enhanced water-use efficiency under both water regimes.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- SlTLFP8 overexpressione0
- Gene
- SlTLFP8e0
- Outcome
- WUEe0
- Direction
- enhancede0
- Qualifier
- under both water-sufficient and water-deficient conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlTLFP8 overexpression significantly enhanced WUE by suppressing transpiration under both water-sufficient and water-deficient conditions.”
t0“SlTLFP8 reduces water loss to improve water‐use efficiency by modulating cell size and stomatal density via endoreduplication”
e1“in tomato.”
Claim · model-written summary
SlTLFP8 overexpression suppressed transpiration.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- SlTLFP8 overexpressione0
- Gene
- SlTLFP8e0
- Outcome
- transpiratione0
- Direction
- suppressinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlTLFP8 overexpression significantly enhanced WUE by suppressing transpiration under both water-sufficient and water-deficient conditions.”
t0“SlTLFP8 reduces water loss to improve water‐use efficiency by modulating cell size and stomatal density via endoreduplication”
e1“in tomato.”
Claim · model-written summary
Overexpression increased leaf epidermal cell size.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- overexpressing SlTLFP8e0
- Gene
- SlTLFP8e0
- Outcome
- leaf epidermal cell sizee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpressing SlTLFP8 significantly increased leaf epidermal cell size and thereby decreased stomatal density 10-20%”
t0“SlTLFP8 reduces water loss to improve water‐use efficiency by modulating cell size and stomatal density via endoreduplication”
e1“in tomato.”
Claim · model-written summary
Overexpression decreased stomatal density 10-20%.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- overexpressing SlTLFP8e0
- Gene
- SlTLFP8e0
- Outcome
- stomatal densitye0
- Direction
- decreasede0
- Value
- 10-20%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpressing SlTLFP8 significantly increased leaf epidermal cell size and thereby decreased stomatal density 10-20%”
t0“SlTLFP8 reduces water loss to improve water‐use efficiency by modulating cell size and stomatal density via endoreduplication”
e1“in tomato.”
Claim · model-written summary
Knockout decreased epidermal cell size.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- SlTLFP8 knockoute0
- Gene
- SlTLFP8e0
- Outcome
- cell sizee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlTLFP8 knockout resulted in decreased cell size and thereby increased stomatal density 20-50%”
t0“SlTLFP8 reduces water loss to improve water‐use efficiency by modulating cell size and stomatal density via endoreduplication”
e1“in tomato.”
Claim · model-written summary
Knockout increased stomatal density 20-50%.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- SlTLFP8 knockoute0
- Gene
- SlTLFP8e0
- Outcome
- stomatal densitye0
- Direction
- increasede0
- Value
- 20-50%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlTLFP8 knockout resulted in decreased cell size and thereby increased stomatal density 20-50%”
t0“SlTLFP8 reduces water loss to improve water‐use efficiency by modulating cell size and stomatal density via endoreduplication”
e1“in tomato.”
Claim · model-written summary
Overexpression and knockout modulated ploidy levels in leaf cells.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- SlTLFP8 overexpression and knockoute0
- Gene
- SlTLFP8e0
- Outcome
- ploidy levels in leaf cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlTLFP8 overexpression and knockout modulated ploidy levels in leaf cells.”
t0“SlTLFP8 reduces water loss to improve water‐use efficiency by modulating cell size and stomatal density via endoreduplication”
e1“in tomato.”
Claim · model-written summary
Cell-cycle gene expression indicated that SlTLFP8 affects cell size and stomatal density through endocycle transition.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- SlTLFP8e0
- Outcome
- cell size and stomatal densitye0
- Qualifier
- through endocycle transitione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Changes in expression of cell cycle related genes also indicated that SlTLFP8 affected cell size and stomatal density through endocycle transition.”
t0“SlTLFP8 reduces water loss to improve water‐use efficiency by modulating cell size and stomatal density via endoreduplication”
e1“in tomato.”
Claim · model-written summary
Altering SlTLFP8 expression did not change photosynthesis.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- altering the expression of SlTLFP8e0
- Gene
- SlTLFP8e0
- Outcome
- photosynthesise0
- Direction
- did not changee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“altering the expression of SlTLFP8 did not change photosynthesis.”
t0“SlTLFP8 reduces water loss to improve water‐use efficiency by modulating cell size and stomatal density via endoreduplication”
e1“in tomato.”
Claim · model-written summary
Biomass was not altered in transgenic lines.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- transgenice0
- Outcome
- biomasse0
- Direction
- not alterede0
- Comparator
- wild type linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“biomass was not altered and there was little difference in fruit yield for transgenic and wild type lines under water-sufficient and water-deficient conditions.”
t0“SlTLFP8 reduces water loss to improve water‐use efficiency by modulating cell size and stomatal density via endoreduplication”
e1“in tomato.”
Claim · model-written summary
Fruit yield differed little between transgenic and wild-type lines across water regimes.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- transgenice0
- Outcome
- fruit yielde0
- Direction
- little differencee0
- Qualifier
- under water-sufficient and water-deficient conditionse0
- Comparator
- wild type linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“biomass was not altered and there was little difference in fruit yield for transgenic and wild type lines under water-sufficient and water-deficient conditions.”
t0“SlTLFP8 reduces water loss to improve water‐use efficiency by modulating cell size and stomatal density via endoreduplication”
e1“in tomato.”
The same questions in other papers
transgenic · disease · Solanum lycopersicum (tomato)Positive and negative results
15 papers in the same direction as this paper · 10 in the opposite direction · 1 reporting no difference from an alternative
- negative
- no effect
- negative
23 papers more in this group
See all 27 papers →transgenic · physiology: photosynthesis · Solanum lycopersicum (tomato)Results agree
2 papers in the same direction as this paper
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.