Abstract
Transgenic tomato (Solanum lycopersicum) plants expressing a fragment of the Sl SDH2-2 gene encoding the iron sulfur subunit of the succinate dehydrogenase protein complex in the antisense orientation under the control of the 35S promoter exhibit an enhanced rate of photosynthesis. The rate of the tricarboxylic acid (TCA) cycle was reduced in these transformants, and there were changes in the levels of metabolites associated with the TCA cycle. Furthermore, in comparison to wild-type plants, carbon dioxide assimilation was enhanced by up to 25% in the transgenic plants under ambient conditions, and mature plants were characterized by an increased biomass. Analysis of additional photosynthetic parameters revealed that the rate of transpiration and stomatal conductance were markedly elevated in the transgenic plants. The transformants displayed a strongly enhanced assimilation rate under both ambient and suboptimal environmental conditions, as well as an elevated maximal stomatal aperture. By contrast, when the Sl SDH2-2 gene was repressed by antisense RNA in a guard cell-specific manner, changes in neither stomatal aperture nor photosynthesis were observed. The data obtained are discussed in the context of the role of TCA cycle intermediates both generally with respect to photosynthetic metabolism and specifically with respect to their role in the regulation of stomatal aperture.
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Claims 9
Claim · model-written summary
Sl SDH2-2 antisense tomato plants showed an enhanced photosynthesis rate.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- antisense orientatione0
- Gene
- Sl SDH2-2e0
- Outcome
- rate of photosynthesise0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic tomato (Solanum lycopersicum) plants expressing a fragment of the Sl SDH2-2 gene encoding the iron sulfur subunit of the succinate dehydrogenase protein complex in the antisense orientation under the control of the 35S promoter exhibit an enhanced rate of photosynthesis.”
Claim · model-written summary
The TCA cycle rate was reduced in the transformants.
Not reportedTaxonComparator
Verbatim
e0“The rate of the tricarboxylic acid (TCA) cycle was reduced in these transformants”
Claim · model-written summary
The transformants had changed levels of TCA cycle metabolites.
Not reportedTaxon
Verbatim
e0“there were changes in the levels of metabolites associated with the TCA cycle”
Claim · model-written summary
Transgenic tomato had up to 25% higher carbon dioxide assimilation than wild type under ambient conditions.
- Treatment
- transgenic plantse0
- Outcome
- carbon dioxide assimilatione0
- Direction
- enhancede0
- Value
- 25%e0
- Qualifier
- ambient conditionse0
- Comparator
- wild-type plantse0
Not reportedTaxon
Verbatim
e0“in comparison to wild-type plants, carbon dioxide assimilation was enhanced by up to 25% in the transgenic plants under ambient conditions”
Claim · model-written summary
Mature transgenic tomato plants had increased biomass.
Not reportedTaxonTreatmentComparator
Verbatim
e0“mature plants were characterized by an increased biomass”
Claim · model-written summary
Transgenic tomato had markedly elevated transpiration and stomatal conductance.
- Treatment
- transgenic plantse0
- Outcome
- rate of transpiration and stomatal conductancee0
- Direction
- elevatede0
Not reportedTaxonComparator
Verbatim
e0“the rate of transpiration and stomatal conductance were markedly elevated in the transgenic plants”
Claim · model-written summary
Transformants had enhanced assimilation under ambient and suboptimal conditions.
- Treatment
- transformantse0
- Outcome
- assimilation ratee0
- Direction
- enhancede0
- Qualifier
- ambient and suboptimal environmental conditionse0
Not reportedTaxonComparator
Verbatim
e0“The transformants displayed a strongly enhanced assimilation rate under both ambient and suboptimal environmental conditions”
Claim · model-written summary
Transformants had elevated maximal stomatal aperture.
Not reportedTaxonTreatmentComparator
Verbatim
e0“as well as an elevated maximal stomatal aperture”
Claim · model-written summary
Guard-cell-specific Sl SDH2-2 antisense repression did not alter stomatal aperture or photosynthesis.
- Treatment
- repressed by antisense RNA in a guard cell-specific mannere0
- Gene
- Sl SDH2-2e0
- Outcome
- stomatal aperture nor photosynthesise0
- Direction
- neithere0
Not reportedTaxonComparator
Verbatim
e0“when the Sl SDH2-2 gene was repressed by antisense RNA in a guard cell-specific manner, changes in neither stomatal aperture nor photosynthesis were observed”
The same questions in other papers
loss of function · physiology: photosynthesis · Solanum lycopersicum (tomato)Positive and negative results
1 paper in the same direction as this paper · 13 in the opposite direction
- negative
- no effect
- no effect
11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.