Abstract
Transgenic tomato (Solanum lycopersicum) plants expressing a fragment of a fumarate hydratase (fumarase) gene in the antisense orientation and exhibiting considerable reductions in the mitochondrial activity of this enzyme show impaired photosynthesis. The rate of the tricarboxylic acid cycle was reduced in the transformants relative to the other major pathways of carbohydrate oxidation and the plants were characterized by a restricted rate of dark respiration. However, biochemical analyses revealed relatively little alteration in leaf metabolism as a consequence of reducing the fumarase activity. That said, in comparison to wild-type plants, CO(2) assimilation was reduced by up to 50% under atmospheric conditions and plants were characterized by a reduced biomass on a whole plant basis. Analysis of further photosynthetic parameters revealed that there was little difference in pigment content in the transformants but that the rate of transpiration and stomatal conductance was markedly reduced. Analysis of the response of the rate of photosynthesis to variation in the concentration of CO(2) confirmed that this restriction was due to a deficiency in stomatal function.
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Claims 9
Claim · model-written summary
Fumarase antisense tomato plants had impaired photosynthesis.
- Taxon
- tomato (Solanum lycopersicum)
Solanum lycopersicume0 - Treatment
- antisense orientatione0
- Gene
- fumarate hydratase (fumarase)e0
- Outcome
- photosynthesise0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic tomato (Solanum lycopersicum) plants expressing a fragment of a fumarate hydratase (fumarase) gene in the antisense orientation and exhibiting considerable reductions in the mitochondrial activity of this enzyme show impaired photosynthesis.”
Claim · model-written summary
The tricarboxylic acid cycle rate was reduced relative to other carbohydrate oxidation pathways.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- transformantse0
- Outcome
- rate of the tricarboxylic acid cyclee0
- Direction
- reducede0
- Comparator
- other major pathways of carbohydrate oxidatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rate of the tricarboxylic acid cycle was reduced in the transformants relative to the other major pathways of carbohydrate oxidation”
t0“tomato plants”
Claim · model-written summary
Dark respiration was restricted.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- rate of dark respiratione0
- Direction
- restrictede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the plants were characterized by a restricted rate of dark respiration”
t0“tomato plants”
Claim · model-written summary
Leaf metabolism changed relatively little after fumarase reduction.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- leafe0
- Gene
- fumarasee0
- Outcome
- leaf metabolisme0
- Direction
- relatively little alteratione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“relatively little alteration in leaf metabolism as a consequence of reducing the fumarase activity”
t0“tomato plants”
Claim · model-written summary
CO2 assimilation fell by up to 50% relative to wild type.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- transformantse1
- Outcome
- CO(2) assimilatione0
- Direction
- reducede0
- Value
- 50%e0
- Qualifier
- under atmospheric conditionse0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in comparison to wild-type plants, CO(2) assimilation was reduced by up to 50% under atmospheric conditions”
e1“little difference in pigment content in the transformants”
t0“tomato plants”
Claim · model-written summary
Whole plant biomass was reduced.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- biomasse0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plants were characterized by a reduced biomass on a whole plant basis”
t0“tomato plants”
Claim · model-written summary
Pigment content differed little in transformants.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- transformantse0
- Outcome
- pigment contente0
- Direction
- little differencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there was little difference in pigment content in the transformants”
t0“tomato plants”
Claim · model-written summary
Transpiration and stomatal conductance were markedly reduced.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- rate of transpiration and stomatal conductancee0
- Direction
- markedly reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the rate of transpiration and stomatal conductance was markedly reduced”
t0“tomato plants”
Claim · model-written summary
Stomatal dysfunction explained restricted photosynthesis.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- restrictione0
- Qualifier
- deficiency in stomatal functione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this restriction was due to a deficiency in stomatal function”
t0“tomato plants”
The same questions in other papers
loss of function · physiology: photosynthesis · Solanum lycopersicum (tomato)Positive and negative results
12 papers in the same direction as this paper · 2 in the opposite direction
- positive
- no effect
- no effect
11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.