Abstract
In Arabidopsis, jasmonate is required for stamen and pollen maturation. Mutants deficient in jasmonate synthesis, such as opr3, are male-sterile but become fertile when jasmonate is applied to developing flower buds. We have used ATH1 oligonucleotide arrays to follow gene expression in opr3 stamens for 22 h following jasmonate treatment. In these experiments, a total of 821 genes were specifically induced by jasmonate and 480 genes were repressed. Comparisons with data from previous studies indicate that these genes constitute a stamen-specific jasmonate transcriptome, with a large proportion (70%) of the genes expressed in the sporophytic tissue but not in the pollen. Bioinformatics tools allowed us to associate many of the induced genes with metabolic pathways that are probably upregulated during jasmonate-induced maturation. Our pathway analysis led to the identification of specific genes within larger families of homologues that apparently encode stamen-specific isozymes. Extensive additional analysis of our dataset identified 13 transcription factors that may be key regulators of the stamen maturation processes triggered by jasmonate. Two of these transcription factors, MYB21 and MYB24, are the only members of subgroup 19 of the R2R3 family of MYB proteins. A myb21 mutant obtained by reverse genetics exhibited shorter anther filaments, delayed anther dehiscence and greatly reduced male fertility. A myb24 mutant was phenotypically wild-type, but production of a myb21myb24 double mutant indicated that introduction of the myb24 mutation exacerbated all three aspects of the myb21 phenotype. Exogenous jasmonate could not restore fertility to myb21 or myb21myb24 mutant plants. Together with the data from transcriptional profiling, these results indicate that MYB21 and MYB24 are induced by jasmonate and mediate important aspects of the jasmonate response during stamen development.
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Claims 13
Claim · model-written summary
Jasmonate induced 821 genes in opr3 stamens.
- Taxon
- Arabidopsis
Arabidopsise0 - Organ / stage
- stamene0
- Treatment
- jasmonatee1
- Outcome
- genese1
- Direction
- inducede1
- Value
- 821 genese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, jasmonate is required for stamen and pollen maturation.”
e1“a total of 821 genes were specifically induced by jasmonate”
Claim · model-written summary
Jasmonate repressed 480 genes in opr3 stamens.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- stamene0
- Treatment
- jasmonatee0
- Outcome
- genese1
- Direction
- repressede1
- Value
- 480 genese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, jasmonate is required for stamen and pollen maturation.”
e1“480 genes were repressed.”
Claim · model-written summary
Seventy percent of the stamen jasmonate transcriptome genes were expressed in sporophytic tissue but not pollen.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- sporophytic tissuee1
- Outcome
- genes expressed in the sporophytic tissue but not in the pollene1
- Value
- 70%e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, jasmonate is required for stamen and pollen maturation.”
e1“these genes constitute a stamen-specific jasmonate transcriptome, with a large proportion (70%) of the genes expressed in the sporophytic tissue but not in the pollen.”
Claim · model-written summary
Pathway analysis identified genes apparently encoding stamen specific isozymes.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- identification of specific genes within larger families of homologues that apparently encode stamen-specific isozymese1
- Comparator
- larger families of homologuese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, jasmonate is required for stamen and pollen maturation.”
e1“Our pathway analysis led to the identification of specific genes within larger families of homologues that apparently encode stamen-specific isozymes.”
Claim · model-written summary
Analysis identified 13 candidate transcription factor regulators of stamen maturation.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- transcription factors that may be key regulators of the stamen maturation processese1
- Value
- 13 transcription factorse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, jasmonate is required for stamen and pollen maturation.”
e1“identified 13 transcription factors that may be key regulators of the stamen maturation processes triggered by jasmonate.”
Claim · model-written summary
MYB21 and MYB24 are the only members of subgroup 19 of the R2R3 MYB family.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- MYB21 and MYB24e1
- Outcome
- only members of subgroup 19 of the R2R3 family of MYB proteinse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, jasmonate is required for stamen and pollen maturation.”
e1“MYB21 and MYB24, are the only members of subgroup 19 of the R2R3 family of MYB proteins.”
Claim · model-written summary
The myb21 mutant had shorter anther filaments.
- Taxon
- ArabidopsisArabidopsise0
- Material
- myb21 mutante1
- Outcome
- anther filamentse1
- Direction
- shortere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, jasmonate is required for stamen and pollen maturation.”
e1“A myb21 mutant obtained by reverse genetics exhibited shorter anther filaments, delayed anther dehiscence and greatly reduced male fertility.”
Claim · model-written summary
The myb21 mutant had delayed anther dehiscence.
- Taxon
- ArabidopsisArabidopsise0
- Material
- myb21 mutante1
- Outcome
- anther dehiscencee1
- Direction
- delayede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, jasmonate is required for stamen and pollen maturation.”
e1“A myb21 mutant obtained by reverse genetics exhibited shorter anther filaments, delayed anther dehiscence and greatly reduced male fertility.”
Claim · model-written summary
The myb21 mutant had greatly reduced male fertility.
- Taxon
- ArabidopsisArabidopsise0
- Material
- myb21 mutante1
- Outcome
- male fertilitye1
- Direction
- greatly reducede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, jasmonate is required for stamen and pollen maturation.”
e1“A myb21 mutant obtained by reverse genetics exhibited shorter anther filaments, delayed anther dehiscence and greatly reduced male fertility.”
Claim · model-written summary
The myb24 mutant was phenotypically wild type.
- Taxon
- ArabidopsisArabidopsise0
- Material
- myb24 mutante1
- Outcome
- phenotypically wild-typee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, jasmonate is required for stamen and pollen maturation.”
e1“A myb24 mutant was phenotypically wild-type”
Claim · model-written summary
Adding myb24 mutation exacerbated all three myb21 phenotypes.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- introduction of the myb24 mutatione1
- Outcome
- all three aspects of the myb21 phenotypee1
- Direction
- exacerbatede1
- Comparator
- myb21 phenotypee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, jasmonate is required for stamen and pollen maturation.”
e1“introduction of the myb24 mutation exacerbated all three aspects of the myb21 phenotype.”
Claim · model-written summary
Exogenous jasmonate did not restore fertility to myb21 mutants.
- Taxon
- ArabidopsisArabidopsise0
- Material
- myb21e1
- Treatment
- Exogenous jasmonatee1
- Outcome
- fertilitye1
- Direction
- could not restoree1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, jasmonate is required for stamen and pollen maturation.”
e1“Exogenous jasmonate could not restore fertility to myb21 or myb21myb24 mutant plants.”
Claim · model-written summary
Exogenous jasmonate did not restore fertility to double mutants.
- Taxon
- ArabidopsisArabidopsise0
- Material
- myb21myb24e1
- Treatment
- Exogenous jasmonatee1
- Outcome
- fertilitye1
- Direction
- could not restoree1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, jasmonate is required for stamen and pollen maturation.”
e1“Exogenous jasmonate could not restore fertility to myb21 or myb21myb24 mutant plants.”
The same questions in other papers
jasmonic acid · molecular: expression · ArabidopsisPositive and negative results
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