Abstract
In spite of the importance of jasmonates (JAs) as plant growth and stress regulators, the molecular components of their signaling pathway remain largely unknown. By means of a genetic screen that exploits the cross talk between ethylene (ET) and JAs, we describe the identification of several new loci involved in JA signaling and the characterization and positional cloning of one of them, JASMONATE-INSENSITIVE1 (JAI1/JIN1). JIN1 encodes AtMYC2, a nuclear-localized basic helix-loop-helix-leucine zipper transcription factor, whose expression is rapidly upregulated by JA, in a CORONATINE INSENSITIVE1-dependent manner. Gain-of-function experiments confirmed the relevance of AtMYC2 in the activation of JA signaling. AtMYC2 differentially regulates the expression of two groups of JA-induced genes. The first group includes genes involved in defense responses against pathogens and is repressed by AtMYC2. Consistently, jin1 mutants show increased resistance to necrotrophic pathogens. The second group, integrated by genes involved in JA-mediated systemic responses to wounding, is activated by AtMYC2. Conversely, Ethylene-Response-Factor1 (ERF1) positively regulates the expression of the first group of genes and represses the second. These results highlight the existence of two branches in the JA signaling pathway, antagonistically regulated by AtMYC2 and ERF1, that are coincident with the alternative responses activated by JA and ET to two different sets of stresses, namely pathogen attack and wounding.
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Claims 10
Claim · model-written summary
Several new jasmonate signaling loci were identified.
- Taxon
- Arabidopsis
Arabidopsist0 - Outcome
- new loci involved in JA signalinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“identification of several new loci involved in JA signaling”
Claim · model-written summary
JIN1 encodes the nuclear transcription factor AtMYC2.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- JIN1e0
- Outcome
- AtMYC2, a nuclear-localized basic helix-loop-helix-leucine zipper transcription factore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“JIN1 encodes AtMYC2, a nuclear-localized basic helix-loop-helix-leucine zipper transcription factor”
Claim · model-written summary
JA rapidly upregulated AtMYC2 expression in a CORONATINE INSENSITIVE1 dependent manner.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- JAe0
- Gene
- AtMYC2e1
- Outcome
- expressione0
- Direction
- upregulatede0
- Qualifier
- CORONATINE INSENSITIVE1-dependent mannere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“whose expression is rapidly upregulated by JA, in a CORONATINE INSENSITIVE1-dependent manner”
e1“JIN1 encodes AtMYC2”
Claim · model-written summary
Gain of function experiments supported a role for AtMYC2 in activating JA signaling.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- AtMYC2e0
- Outcome
- activation of JA signalinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“Gain-of-function experiments confirmed the relevance of AtMYC2 in the activation of JA signaling.”
Claim · model-written summary
AtMYC2 repressed the JA induced pathogen defense gene group.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- AtMYC2e0
- Outcome
- genes involved in defense responses against pathogense0
- Direction
- repressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“genes involved in defense responses against pathogens and is repressed by AtMYC2”
Claim · model-written summary
jin1 mutants had increased resistance to necrotrophic pathogens.
- Taxon
- ArabidopsisArabidopsist0
- Material
- jin1 mutantse0
- Interactor
- necrotrophic pathogense0pathogen
- Outcome
- resistance to necrotrophic pathogense0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“jin1 mutants show increased resistance to necrotrophic pathogens”
Claim · model-written summary
AtMYC2 activated the JA mediated systemic wound response gene group.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- AtMYC2e0
- Outcome
- genes involved in JA-mediated systemic responses to woundinge0
- Direction
- activatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“genes involved in JA-mediated systemic responses to wounding, is activated by AtMYC2”
Claim · model-written summary
ERF1 activated the pathogen defense gene group.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- Ethylene-Response-Factor1 (ERF1)e0
- Outcome
- expression of the first group of genese0
- Direction
- positively regulatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“Ethylene-Response-Factor1 (ERF1) positively regulates the expression of the first group of genes”
Claim · model-written summary
ERF1 repressed the systemic wound response gene group.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- Ethylene-Response-Factor1 (ERF1)e0
- Outcome
- the seconde0
- Direction
- repressese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“Ethylene-Response-Factor1 (ERF1) positively regulates the expression of the first group of genes and represses the second”
Claim · model-written summary
The authors inferred two antagonistically regulated JA signaling branches.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- AtMYC2 and ERF1e0
- Outcome
- two branches in the JA signaling pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“two branches in the JA signaling pathway, antagonistically regulated by AtMYC2 and ERF1”
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