Abstract
Callus cultures were initiated from epicotyls of field pea (Pisum sativum, L. cv. Solara). Growing cells were exposed to increasing concentrations of NaCl on modified PC L 2 basal medium. A callus culture were exposed to different concentration of NaCl from 0 -400 mol m -3 . The concentration of 400 mol m -3 NaCl proved completely inhibitory to the growth of calli. After incubation the cell suspensions for 28 days cells which tolerate this concentration of NaCl grew to form cell clones. Selected clones were characterized with regard to their growth behavior, K + , Na + and free proline contents when callus grown under stress as well as on normal media were compared with the normal sensitive callus. Under normal conditions, however, the amounts of free proline in selected and non selected calli were compared. Salt selected cells accumulated higher levels of free proline under NaCl stress compared to wild type cells. The selected callus was capable of growing on medium containing NaCl at the inhibitory concentration. The K + content of the selected callus was lower in the case of the NaCl medium than for the normal medium. However, with increasing salinity concentrations however, the selected clones maintained higher K + and Na + levels, compared with the wild type cells.
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Claims 5
Claim · model-written summary
The concentration of 400 mol m -3 NaCl completely inhibited the growth of pea callus.
- Taxon
- Pisum sativum
Lathyrus oleraceuse0 - Material
- cv. Solarae0
- Organ / stage
- calluse0
- Treatment
- 400 mol m -3 NaCle1
- Outcome
- growth of callie1
- Direction
- inhibitorye1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus cultures were initiated from epicotyls of field pea (Pisum sativum, L. cv. Solara)”
e1“The concentration of 400 mol m -3 NaCl proved completely inhibitory to the growth of calli”
Claim · model-written summary
Salt-selected callus grew on medium containing NaCl at the inhibitory concentration.
- Taxon
- Pisum sativumLathyrus oleraceuse0
- Treatment
- medium containing NaCl at the inhibitory concentratione2
- Outcome
- capable of growinge2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus cultures were initiated from epicotyls of field pea (Pisum sativum, L. cv. Solara)”
e2“The selected callus was capable of growing on medium containing NaCl at the inhibitory concentration”
Claim · model-written summary
Salt-selected cells accumulated higher levels of free proline under NaCl stress than wild type cells.
- Taxon
- Pisum sativumLathyrus oleraceuse0
- Treatment
- NaCl stresse3
- Outcome
- levels of free prolinee3
- Direction
- highere3
- Comparator
- wild type cellse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus cultures were initiated from epicotyls of field pea (Pisum sativum, L. cv. Solara)”
e3“Salt selected cells accumulated higher levels of free proline under NaCl stress compared to wild type cells”
Claim · model-written summary
The K+ content of the selected callus was lower in NaCl medium than in normal medium.
- Taxon
- Pisum sativumLathyrus oleraceuse0
- Treatment
- NaCl mediume4
- Outcome
- K + contente4
- Direction
- lowere4
- Comparator
- normal mediume4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus cultures were initiated from epicotyls of field pea (Pisum sativum, L. cv. Solara)”
e4“The K + content of the selected callus was lower in the case of the NaCl medium than for the normal medium”
Claim · model-written summary
With increasing salinity, the selected clones maintained higher K+ and Na+ levels than wild type cells.
- Taxon
- Pisum sativumLathyrus oleraceuse0
- Treatment
- increasing salinity concentrationse5
- Outcome
- K + and Na + levelse5
- Direction
- highere5
- Comparator
- wild type cellse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus cultures were initiated from epicotyls of field pea (Pisum sativum, L. cv. Solara)”
e5“However, with increasing salinity concentrations however, the selected clones maintained higher K + and Na + levels, compared with the wild type cells”
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