Paper · 2023 · Horticulturae

In Vitro Screening for Salinity Tolerance in Garden Pea (Pisum sativum L.)

Stanislava Grozeva; Slavka Kalapchieva; Ivanka Tringovska

6
claims
16
verbatim quotes
11
citations
2
research areas
✓
Open access · cc-by

Abstract

Soil salinity is one of the abiotic stress factors gaining importance in recent years due to the changing climate and rising temperatures. This possesses a serious risk to food security worldwide and a range of adaptations and mitigation strategies are required. Crop improvement through breeding is a possible solution to overcome salinity stress. In this respect, a study was designed to screen a collection from garden pea accessions to salinity tolerance in in vitro conditions. We analyzed the effects of four salinity levels (0, 50, 100, and 200 mM NaCl) on seed germination, seedling emergence, shoot and root lengths, and plant fresh weight in 22 garden pea genotypes. Data showed that more of the pea genotypes are able to tolerate 50 mM NaCl concentration. The increasing of salt levels to 100 and 200 mM NaCl caused a significant decrease in germination and reduced the length and weight of shoots and roots. Among the genotypes, varieties Uspeh 72, Paldin, and Flora 6 were highly sensitive to salinity stress, while varieties Prometey, Musala, and Zornitsa were distinguished as more tolerant. The results depicted that salinity treatments had a more negative effect on plant growth than on seed germination, which is probably due to the in vitro conditions in which the experiments were conducted. The studied accessions of garden pea were sensitive to salt stress; nevertheless, some tolerant accessions were identified.

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Claims 6

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

More pea genotypes tolerated 50 mM NaCl.

Treatment
50 mM NaCle0
Outcome
tolerate 50 mM NaCl concentratione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“more of the pea genotypes are able to tolerate 50 mM NaCl concentration”

✓ located verbatim in the source · abstract, characters 694–765 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

At 100 and 200 mM NaCl, germination significantly decreased.

Treatment
100 and 200 mM NaCle0t0e1
Outcome
germinatione0t0e1
Direction
decreasee0t0e1
Comparator
50 mM NaCle0t0e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The increasing of salt levels to 100 and 200 mM NaCl caused a significant decrease in germination”

✓ located verbatim in the source · abstract, characters 767–864 · show in text ↑
t0

“Garden Pea (Pisum sativum L.)”

✓ located verbatim in the source · title, characters 45–74 · show in text ↑
e1

“more of the pea genotypes are able to tolerate 50 mM NaCl concentration”

✓ located verbatim in the source · abstract, characters 694–765 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

At 100 and 200 mM NaCl, shoot and root length and weight decreased.

Organ / stage
shoots and rootse0t0e1
Treatment
100 and 200 mM NaCle0t0e1
Outcome
length and weight of shoots and rootse0t0e1
Direction
reducede0t0e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The increasing of salt levels to 100 and 200 mM NaCl caused a significant decrease in germination and reduced the length and weight of shoots and roots”

✓ located verbatim in the source · abstract, characters 767–918 · show in text ↑
t0

“Garden Pea (Pisum sativum L.)”

✓ located verbatim in the source · title, characters 45–74 · show in text ↑
e1

“more of the pea genotypes are able to tolerate 50 mM NaCl concentration”

✓ located verbatim in the source · abstract, characters 694–765 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Uspeh 72, Paldin, and Flora 6 were highly sensitive to salinity.

Material
Uspeh 72, Paldin, and Flora 6e0t0e1
Outcome
highly sensitive to salinity stresse0t0e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“varieties Uspeh 72, Paldin, and Flora 6 were highly sensitive to salinity stress”

✓ located verbatim in the source · abstract, characters 941–1021 · show in text ↑
t0

“Garden Pea (Pisum sativum L.)”

✓ located verbatim in the source · title, characters 45–74 · show in text ↑
e1

“more of the pea genotypes are able to tolerate 50 mM NaCl concentration”

✓ located verbatim in the source · abstract, characters 694–765 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Prometey, Musala, and Zornitsa were more tolerant to salinity.

Material
Prometey, Musala, and Zornitsae0t0e1
Outcome
more tolerante0t0e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“varieties Prometey, Musala, and Zornitsa were distinguished as more tolerant”

✓ located verbatim in the source · abstract, characters 1029–1105 · show in text ↑
t0

“Garden Pea (Pisum sativum L.)”

✓ located verbatim in the source · title, characters 45–74 · show in text ↑
e1

“more of the pea genotypes are able to tolerate 50 mM NaCl concentration”

✓ located verbatim in the source · abstract, characters 694–765 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

Salinity affected growth more negatively than germination.

Treatment
salinity treatmentse0t0e1
Outcome
plant growthe0t0e1
Direction
more negativee0t0e1
Comparator
seed germinatione0t0e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“salinity treatments had a more negative effect on plant growth than on seed germination”

✓ located verbatim in the source · abstract, characters 1133–1220 · show in text ↑
t0

“Garden Pea (Pisum sativum L.)”

✓ located verbatim in the source · title, characters 45–74 · show in text ↑
e1

“more of the pea genotypes are able to tolerate 50 mM NaCl concentration”

✓ located verbatim in the source · abstract, characters 694–765 · show in text ↑

sodium chloride · growth · Lathyrus oleraceus (pea)Results differ

28 papers: 4 positive, 24 negative or no effect

23 papers in the same direction as this paper · 4 in the opposite direction

  1. negative
  2. no effect
  3. negative

24 papers more in this group

See all 28 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.