Paper · 2025 · Seeds

Priming ‘Santa Isabel’ Pea (Pisum sativum L.) Seeds with NaCl and H2O2 as a Strategy to Promote Germination

Javier Giovanni Álvarez-Herrera; Julián Stiven Lozano; Oscar Humberto Alvarado-Sanabria

7
claims
22
verbatim quotes
1
citations
2
research areas
✓
Open access · cc-by

Abstract

Peas possess significant nutritional properties due to their high protein levels, carbohydrates, fiber, and vitamins. Increased climate variability can lead to water stress in crops like peas. Therefore, priming plants through seed priming is a technique that has proven effective as a pre-conditioning method for plants to cope with more severe future stresses. Different doses and soaking times of ‘Santa Isabel’ pea seeds in NaCl and H2O2 were evaluated to enhance and promote germination. Two experiments were conducted under controlled conditions (average temperature 15.8 °C) through a completely randomized design with a 4 × 3 factorial arrangement, comprising 12 treatments in each trial. In the first trial, NaCl doses (0, 50, 100, or 150 mM) and the soaking time of the seeds in NaCl (12, 24, or 36 h) were examined. In the second trial, H2O2 doses (0, 20, 40, or 60 mM) were tested with the same imbibition times. The 50 mM NaCl dose at 24 h demonstrated the best values for germination rate index, mean germination time, germination rate (GR), and germination potential (GP). Seed imbibition for 24 h in NaCl, as well as in H2O2, is the ideal time to achieve the best GR and GP. The dry mass of leaf and stipule recorded the highest values with a 60 mM dose of H2O2 and 24 h of imbibition. An application of 150 mM NaCl resulted in the highest values of germinated seed dry mass, while causing lower dry mass in roots, stems, leaves, and stipules; however, it maintained similar total dry mass values.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 7

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The 50 mM NaCl dose at 24 h gave the best germination rate index and mean germination time.

Material
Santa Isabele0
Treatment
50 mM NaCl dose at 24 he1
Outcome
germination rate index, mean germination timee1
Direction
best valuese1
Setting
controlled conditionse6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“‘Santa Isabel’ pea seeds in NaCl and H2O2”

✓ located verbatim in the source · abstract, characters 400–441 · show in text ↑
e1

“The 50 mM NaCl dose at 24 h demonstrated the best values for germination rate index, mean germination time, germination rate (GR), and germination potential (GP)”

✓ located verbatim in the source · abstract, characters 925–1086 · show in text ↑
e6

“Two experiments were conducted under controlled conditions (average temperature 15.8 °C)”

✓ located verbatim in the source · abstract, characters 493–581 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The 50 mM NaCl dose at 24 h gave the best germination rate (GR) and germination potential (GP).

Material
Santa Isabele0
Treatment
50 mM NaCl dose at 24 he1
Outcome
germination rate (GR), and germination potential (GP)e1
Direction
best valuese1
Setting
controlled conditionse6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“‘Santa Isabel’ pea seeds in NaCl and H2O2”

✓ located verbatim in the source · abstract, characters 400–441 · show in text ↑
e1

“The 50 mM NaCl dose at 24 h demonstrated the best values for germination rate index, mean germination time, germination rate (GR), and germination potential (GP)”

✓ located verbatim in the source · abstract, characters 925–1086 · show in text ↑
e6

“Two experiments were conducted under controlled conditions (average temperature 15.8 °C)”

✓ located verbatim in the source · abstract, characters 493–581 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Seed imbibition for 24 h in NaCl and in H2O2 is the ideal time for the best GR and GP.

Material
Santa Isabele0
Treatment
Seed imbibition for 24 h in NaCl, as well as in H2O2e2
Outcome
GR and GPe2
Direction
beste2
Setting
controlled conditionse6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“‘Santa Isabel’ pea seeds in NaCl and H2O2”

✓ located verbatim in the source · abstract, characters 400–441 · show in text ↑
e2

“Seed imbibition for 24 h in NaCl, as well as in H2O2, is the ideal time to achieve the best GR and GP”

✓ located verbatim in the source · abstract, characters 1088–1189 · show in text ↑
e6

“Two experiments were conducted under controlled conditions (average temperature 15.8 °C)”

✓ located verbatim in the source · abstract, characters 493–581 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

A 60 mM H2O2 dose with 24 h imbibition gave the highest leaf and stipule dry mass.

Material
Santa Isabele0
Treatment
60 mM dose of H2O2 and 24 h of imbibitione3
Outcome
dry mass of leaf and stipulee3
Direction
highest valuese3
Setting
controlled conditionse6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“‘Santa Isabel’ pea seeds in NaCl and H2O2”

✓ located verbatim in the source · abstract, characters 400–441 · show in text ↑
e3

“The dry mass of leaf and stipule recorded the highest values with a 60 mM dose of H2O2 and 24 h of imbibition”

✓ located verbatim in the source · abstract, characters 1191–1300 · show in text ↑
e6

“Two experiments were conducted under controlled conditions (average temperature 15.8 °C)”

✓ located verbatim in the source · abstract, characters 493–581 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The 150 mM NaCl treatment gave the highest germinated seed dry mass.

Material
Santa Isabele0
Treatment
150 mM NaCle4
Outcome
germinated seed dry masse4
Direction
highest valuese4
Setting
controlled conditionse6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“‘Santa Isabel’ pea seeds in NaCl and H2O2”

✓ located verbatim in the source · abstract, characters 400–441 · show in text ↑
e4

“An application of 150 mM NaCl resulted in the highest values of germinated seed dry mass, while causing lower dry mass in roots, stems, leaves, and stipules”

✓ located verbatim in the source · abstract, characters 1302–1458 · show in text ↑
e6

“Two experiments were conducted under controlled conditions (average temperature 15.8 °C)”

✓ located verbatim in the source · abstract, characters 493–581 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

The 150 mM NaCl treatment gave lower dry mass in roots, stems, leaves and stipules.

Material
Santa Isabele0
Treatment
150 mM NaCle4
Outcome
dry mass in roots, stems, leaves, and stipulese4
Direction
lowere4
Setting
controlled conditionse6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“‘Santa Isabel’ pea seeds in NaCl and H2O2”

✓ located verbatim in the source · abstract, characters 400–441 · show in text ↑
e4

“An application of 150 mM NaCl resulted in the highest values of germinated seed dry mass, while causing lower dry mass in roots, stems, leaves, and stipules”

✓ located verbatim in the source · abstract, characters 1302–1458 · show in text ↑
e6

“Two experiments were conducted under controlled conditions (average temperature 15.8 °C)”

✓ located verbatim in the source · abstract, characters 493–581 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

The 150 mM NaCl treatment maintained similar total dry mass.

Material
Santa Isabele0
Treatment
150 mM NaCle4
Outcome
total dry masse5
Direction
similare5
Setting
controlled conditionse6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“‘Santa Isabel’ pea seeds in NaCl and H2O2”

✓ located verbatim in the source · abstract, characters 400–441 · show in text ↑
e4

“An application of 150 mM NaCl resulted in the highest values of germinated seed dry mass, while causing lower dry mass in roots, stems, leaves, and stipules”

✓ located verbatim in the source · abstract, characters 1302–1458 · show in text ↑
e5

“it maintained similar total dry mass values”

✓ located verbatim in the source · abstract, characters 1469–1512 · show in text ↑
e6

“Two experiments were conducted under controlled conditions (average temperature 15.8 °C)”

✓ located verbatim in the source · abstract, characters 493–581 · show in text ↑

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.